Effect of two crosslinking methods on the physicochemical and biological properties of the collagen-chitosan scaffolds

Reyna-Urrutia, Víctor A.; Mata-Haro, Verónica; Cauich-Rodríguez, Juan Valerio; Herrera‐Kao, Wilberth; Cervantes‐Uc, José Manuel

doi:10.1016/j.eurpolymj.2019.05.010

Cited by 58 publications

(39 citation statements)

References 44 publications

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“…CHT can be crosslinked by a variety of methods, such as the use of glutaraldehyde, formaldehyde, tripolyphosphate, genipin and/or vanillin. 57 However, in this experimental work, no crosslinking step was performed in the fabrication of the CHT membrane. Instead, the CHT powder was dissolved in an acidic solution (acetic acid, in which the NH 2 groups become protonated (NH 3+ )).…”

Section: Discussionmentioning

confidence: 99%

Enhanced performance of chitosan/keratin membranes with potential application in peripheral nerve repair

Carvalho

Costa

et al. 2019

Biomater. Sci.

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Section: Discussionmentioning

confidence: 99%

Enhanced performance of chitosan/keratin membranes with potential application in peripheral nerve repair

Carvalho

Costa

et al. 2019

Biomater. Sci.

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“…After photo crosslinking with riboflavin, a slight increase to the porosity of the scaffold was observed in accordance with the SEM micrographs. This can be attributed to the fact that cross-linking (either physical or chemical) affects the scaffold's pore size, since the shrinkage of the hydrogel structure is inhibited due to a rearrangement of collagen chains [65,66]. The porosity of the non-crosslinked scaffold was estimated to be 54%, a property that reached 61% after the cross-linking process ( Table 2).…”

Section: Micro-computed Tomography (µ-Ct)mentioning

confidence: 99%

Mechanical Enhancement of Cytocompatible 3D Scaffolds, Consisting of Hydroxyapatite Nanocrystals and Natural Biomolecules, Through Physical Cross-Linking

et al. 2020

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Bioinspired scaffolds mimicking natural bone-tissue properties holds great promise in tissue engineering applications towards bone regeneration. Within this work, a way to reinforce mechanical behavior of bioinspired bone scaffolds was examined by applying a physical crosslinking method. Scaffolds consisted of hydroxyapatite nanocrystals, biomimetically synthesized in the presence of collagen and l-arginine. Scaffolds were characterized by X-ray diffraction, Fourier transform infrared spectroscopy, scanning electron microscopy (SEM), microcomputed tomography, and nanoindentation. Results revealed scaffolds with bone-like nanostructure and composition, thus an inherent enhanced cytocompatibility. Evaluation of porosity proved the development of interconnected porous network with bimodal pore size distribution. Mechanical reinforcement was achieved through physical crosslinking with riboflavin irradiation, and nanoindentation tests indicated that within the experimental conditions of 45% humidity and 37 °C, photo-crosslinking led to an increase in the scaffold’s mechanical properties. Elastic modulus and hardness were augmented, and specifically elastic modulus values were doubled, approaching equivalent values of trabecular bone. Cytocompatibility of the scaffolds was assessed using MG63 human osteosarcoma cells. Cell viability was evaluated by double staining and MTT assay, while attachment and morphology were investigated by SEM. The results suggested that scaffolds provided a cell friendly environment with high levels of viability, thus supporting cell attachment, spreading and proliferation.

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“…Covalently crosslinked chitosan hydrogels are very effective in drug delivery systems [15,16]; however, they may show some drawbacks when used as, for example, toxic crosslinkers or initiators. The use of physical hydrogels makes it possible to overcome such limitations [17]. Among different approaches, and taking advantage of the positively charged chitosan, we decided to investigate the formation of CS-DNA and CS-P coacervate hydrogels.…”

Section: Introductionmentioning

confidence: 99%

Chitosan-Based Coacervate Polymers for Propolis Encapsulation: Release and Cytotoxicity Studies

Sato

Mello

Vasconcellos

et al. 2020

IJMS

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Chitosan-DNA (CS-DNA) and Chitosan-Pectin (CS-P) hydrogels were formulated as a sustained drug delivery carrier for drug delivery. For this, hydrogels were prepared by emulsion technique: mixing aqueous phase of the CS and DNA or P solution with benzyl alcohol using a high-performance dispersing instrument. Green Propolis (GP) was incorporated by imbibition: hydrogels were placed in GP aqueous solution (70 µg/mL) for 2 h. The specimens were freeze-dried and then characterized using different techniques. In vitro cell viability and morphology were also performed using the MG63 cell line. The presence of P was evidenced by the occurrence of a strong band at 1745 cm−1, also occurring in the blend. DNA and CS-DNA showed a strong band at 1650 cm−1, slightly shifted from the chitosan band. The sorption of GP induced a significant modification of the gel surface morphology and some phase separation occurs between chitosan and DNA. Drug release kinetics in water and in saliva follow a two-step mechanism. Significant biocompatibility revealed that these hydrogels were non-toxic and provided acceptable support for cell survival. Thus, the hydrogel complexation of chitosan with DNA and with Pectin provides favorable micro-environment for cell growth and is a viable alternative drug delivery system for Green Propolis.

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Effect of two crosslinking methods on the physicochemical and biological properties of the collagen-chitosan scaffolds

Cited by 58 publications

References 44 publications

Enhanced performance of chitosan/keratin membranes with potential application in peripheral nerve repair

Enhanced performance of chitosan/keratin membranes with potential application in peripheral nerve repair

Mechanical Enhancement of Cytocompatible 3D Scaffolds, Consisting of Hydroxyapatite Nanocrystals and Natural Biomolecules, Through Physical Cross-Linking

Chitosan-Based Coacervate Polymers for Propolis Encapsulation: Release and Cytotoxicity Studies

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