Effect of two cytokinins and a growth inhibitor on the in vitro tuberization of two genotypes of Solanum tuberosum L. cvs. Atlantic and Alpha

García-García, José Antonio; Azofeifa-Bolaños, José Bernal; Solano-Campos, Frank; Orozco-Rodríguez, Rafael

doi:10.15359/ru.33-2.1

Cited by 3 publications

(1 citation statement)

References 37 publications

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“…The positive effects of cytokinin on potato tuberization has also been reported by [9] and [10]. Furthermore, in vitro experiment showed that applying a single plant growth regulator (PGR), has been reported to be less effective in inducing tuber formation than applying the combination of PGR and retardant, especially at high temperature [11].…”

Section: Introductionmentioning

confidence: 87%

Inducing potato tuber formation at low elevation of tropical region by foliar spray of PGR mixtures at different application times

et al. 2023

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Growing potato crops at low elevation in the tropical region may face many problems related to high temperature, such as an increase in endogen GA3 levels, causing the inhibition of tuber formation. Suppressing GA3 biosynthesis, by applying a mixture of plant growth regulators (PGRs), is of important. The objectives of this experiment were to study the best PGR mixtures, PGR application time, and their interaction in promoting tuber formation at low altitude of tropical areas in Indonesia. The experiment used a completely randomized design arranged in factorial (2 factors, 3 replications). The first factor was a mix of plant growth regulator solution (M), consisting of 4 levels, which were: M0 = No PGR; M1 = 10 ppm 2,4-D + 100 ppm Daminozide, M2 = M1 +10 ppm BAP, M3 = M1 + 25 ppm COU, and M4 = M1+ 500 ppm PBZ. The second factor was application time of the solutions (T), consisting of 3 levels, which were: T1 = 3, 4, 5, 6 weeks after emergence (WAE), T2 = 3, 4, 5 WAE, and T3 = 3, 4 WAE. The results showed that all of the PGR mixtures promoted tuber formation with 10 ppm 2,4-D + 100 ppm Daminozide + 500 ppm Paclobutrazol was found as the best PGR mixture. Moreover, the best time to spray the mixture was 3,4,5,6 WAE. Finally, the best interaction between PGR and application time was10 ppm 2,4-D + 100 ppm Daminozide + 10 ppm BAP applied at 3,4,5 WAE.

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Section: Introductionmentioning

confidence: 87%

Inducing potato tuber formation at low elevation of tropical region by foliar spray of PGR mixtures at different application times

et al. 2023

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Development of aerial and belowground tubers in potato is governed by photoperiod and epigenetic mechanism

et al. 2021

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Plants exhibit diverse developmental plasticity and modulate growth responses under various environmental conditions. Potato (Solanum tuberosum), a modified stem and an important food crop, serves as a substantial portion of the world’s subsistence food supply. In the past two decades, crucial molecular signals have been identified that govern the tuberization (potato development) mechanism. Interestingly, microRNA156 overexpression in potato provided the first evidence for induction of profuse aerial stolons and tubers from axillary-meristems under short-day photoperiod. A similar phenotype was noticed for overexpression of epigenetic modifiers - MUTICOPY SUPRESSOR OF IRA1 (StMSI1) or ENAHNCER OF ZESTE 2 (StE[z]2), and knockdown of B-CELL SPECIFIC MOLONEY MURINE LEUKEMIA VIRUS INTEGRATION SITE 1 (StBMI1). This striking phenotype represents a classic example of modulation of plant architecture and developmental plasticity. Differentiation of a stolon to a tuber or a shoot under in vitro or in vivo conditions symbolizes another example of organ level plasticity and dual fate acquisition in potato. Stolon-to-tuber transition is governed by short-day photoperiod, mobile RNAs/proteins, phytohormones, a plethora of small RNAs and their targets. Recent studies show that polycomb group proteins control microRNA156, phytohormone metabolism/transport/signalling, and key tuberization genes through histone modifications to govern tuber development. Our comparative analysis of differentially expressed genes between the overexpression lines of StMSI1, StBEL5 (BEL1-LIKE transcription factor) and POTH15 (POTATO HOMEOBOX 15 transcription factor) revealed >1000 common genes, indicative of a mutual gene regulatory network potentially involved in the formation of aerial and belowground tubers. In this review, in addition to key tuberization factors, we highlight the role of photoperiod and epigenetic mechanism that regulates the development of aerial and belowground tubers in potato.

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Induction of Microtubing of New Promising Potato Varieties in Aseptic Culture

Oves¹,

Gaitova²,

Shishkina³

2021

Vestnik of Kazan State Agrarian University

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The studies were carried out with the aim of optimizing microtubing of promising potato varieties in aseptic culture. The experiments studied such factors as the use of vessels of various sizes (test tubes with a diameter of 25 mm and plastic containers 18x18 cm, into which 10 ml of agar and 400 ml of liquid nutrient medium, respectively, were poured, respectively), the density of planting plants in containers (40, 60 and 80 stem explants), the composition of the nutrient medium during ontogenesis, cultivation of mini-tubers from in vitro microplants (control) and microtubers (≥ 0.9 cm and 0.5 ... 0.9 cm in size). When studying the possibility of modifying the nutrient medium based on the Murashige-Skoog recipe to induce tuberization in one variant, the sucrose concentration during growth was changed from 2% before the formation of four internodes by 8% after this phase, the kinetin content during the entire observation period was 0.5 mg/l. In the second variant, microplants were kept on a medium with 6% sucrose and 0.25 mg/l kinetin throughout ontogenesis. The highest yield of microtubers of the standard fraction (27 ... 94%) with a multiplication factor of 0.8 ... 2.7 pcs/plant was noted in the variant with 60 cuttings placed in a container. When grown in test tubes with a change of medium, 1.0 ... 1.5 microtubers were collected per plant with a standard fraction yield of 64 ... 78%. The use of container technology with a similar alternation of nutrient media increased the yield of the standard fraction in most of the studied varieties to 75 ... 86%. In variants with a constant sucrose content in the nutrient medium (6%), a very low multiplication factor was noted, which did not compensate for a sufficiently high yield of the standard fraction, regardless of the laboratory vessel used. The multiplication factor of test tube microplants during planting in the ground was higher than when planting microtubers, with a high yield of the standard fraction

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Effect of two cytokinins and a growth inhibitor on the in vitro tuberization of two genotypes of Solanum tuberosum L. cvs. Atlantic and Alpha

Cited by 3 publications

References 37 publications

Inducing potato tuber formation at low elevation of tropical region by foliar spray of PGR mixtures at different application times

Inducing potato tuber formation at low elevation of tropical region by foliar spray of PGR mixtures at different application times

Development of aerial and belowground tubers in potato is governed by photoperiod and epigenetic mechanism

Induction of Microtubing of New Promising Potato Varieties in Aseptic Culture

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