Effect of ultrafine-grained titanium surfaces on adhesion of bacteria

Abstract

The influence of the ultrafine crystallinity of commercial purity grade 2 (as-received) titanium and titanium modified by equal channel angular pressing (modified titanium) on bacterial attachment was studied. A topographic profile analysis of the surface of the modified titanium revealed a complex morphology of the surface. Its prominent micro- and nano-scale features were 100-200-nm-scale undulations with 10-15 microm spacing. The undulating surfaces were nano-smooth, with height variations not exceeding 5-10 nm. These surface topography characteristics were distinctly different from those of the as-received samples, where broad valleys (up to 40-60 microm) were detected, whose inner surfaces exhibited asperities approximately 100 nm in height spaced at 1-2 microm. It was found that each of the three bacteria strains used in this study as adsorbates, viz. Staphylococcus aureus CIP 68.5, Pseudomonas aeruginosa ATCC 9025 and Escherichia coli K12, responded differently to the two types of titanium surfaces. Extreme grain refinement by ECAP resulted in substantially increased numbers of cells attached to the surface compared to as-received titanium. This enhanced degree of attachment was accompanied with an increased level of extracellular polymeric substances (EPS) production by the bacteria.

Antibacterial Properties of hLf1–11 Peptide onto Titanium Surfaces: A Comparison Study Between Silanization and Surface Initiated Polymerization

“…roughness) on material surfaces are also crucial in cell and tissue responses to biomaterials. Indeed, surface roughness has shown to alter fibroblast proliferation on aluminiun, titanium and titanium alloys substrates [60][61][62] , and strongly influence bacterial attachment to nitinol, titanium and glass surfaces [63][64][65][66] . Overall, the three functionalization strategies displayed the same value of R a (~ 30 nm; Table 2), slightly increasing the original R a of control samples (~ 25 nm; Table 2).…”

The Effect of Polyterpenol Thin Film Surfaces on Bacterial Viability and Adhesion

“…In cases where the surface features were significantly smaller than the bacterial size, the contact area was reduced, resulting in a reduced extend of binding [15]. The level of extracellular polymeric substance (EPS) production and bacterial cell morphology have also been shown to be affected by the surface architecture [11,16,17]. Nano-patterning of gold surfaces has been demonstrated to enhance the localized attachment of P. fluorescens in the trenches of the surfaces compared to that obtained using native gold surfaces, with cells showing limited EPS production and reduced cell size compare to those attached onto non-nano-patterned surfaces [11].…”

Fructose-enhanced reduction of bacterial growth on nanorough surfaces

“…11,[15][16][17] Recently, many studies have shown that changes in the surface topography at the nanometer level can interfere with mammalian cell as well as bacterial cell functions. 4,[18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33] Conventional biomaterials do not have nanoscale roughness. Nanoscale materials provide roughness and dimensions at the nano level which might influence bacteria behavior.…”