Effect of ultrasound and heat on percutaneous absorption of <scp>l</scp>‐ascorbic acid: human <i>in vitro</i> studies on Franz cell and Petri dish systems

Jung, Eui Chang; Zhu, Hong; Zou, Ying; Elmahdy, Akram; Cao, Yachao; Hui, Xiaoying; Maibach, Howard

doi:10.1111/ics.12350

Cited by 10 publications

(7 citation statements)

References 17 publications

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“…p53 activation by UVB was not seen in the REK organotypic model (Bart et al 2014 ), a result that corresponds to that in human skin in vivo (Enk et al 2004 ). Among the known negative regulators of CLCA2 expression is the transcription factor Fra1 (Zhao et al 2014 ), whose mRNA expression and protein stability is increased by UVB in keratinocytes (Hopper et al 2009 ; Jung et al 2016 ; Silvers and Bowden 2002 ). Accordingly, several other Fra1 targets, such as TGFβ and keratins 10 and 16 (Benhadou et al 2020 ; Zolotarenko et al 2018 ), are affected by UVB in REK organotypic cultures (Bart et al 2014 ; Rauhala et al 2015 ).…”

Section: Resultsmentioning

confidence: 99%

The calcium-activated chloride channel-associated protein rCLCA2 is expressed throughout rat epidermis, facilitates apoptosis and is downmodulated by UVB

Hämäläinen

Bart

Takabe

et al. 2021

Histochem Cell Biol

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The rodent chloride channel regulatory proteins mCLCA2 and its porcine and human homologues pCLCA2 and hCLCA2 are expressed in keratinocytes but their localization and significance in the epidermis have remained elusive. hCLCA2 regulates cancer cell migration, invasion and apoptosis, and its loss predicts poor prognosis in many tumors. Here, we studied the influences of epidermal maturation and UV-irradiation (UVR) on rCLCA2 (previous rCLCA5) expression in cultured rat epidermal keratinocytes (REK) and correlated the results with mCLCA2 expression in mouse skin in vivo. Furthermore, we explored the influence of rCLCA2 silencing on UVR-induced apoptosis. rClca2 mRNA was strongly expressed in REK cells, and its level in organotypic cultures remained unchanged during the epidermal maturation process from a single cell layer to fully differentiated, stratified cultures. Immunostaining confirmed its uniform localization throughout the epidermal layers in REK cultures and in rat skin. A single dose of UVR modestly downregulated rClca2 expression in organotypic REK cultures. The immunohistochemical staining showed that CLCA2 localized in basal and spinous layers also in mouse skin, and repeated UVR induced its partial loss. Interestingly, silencing of rCLCA2 reduced the number of apoptotic cells induced by UVR, suggesting that by facilitating apoptosis, CLCA2 may protect keratinocytes against the risk of malignancy posed by UVB-induced corrupt DNA.

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Section: Resultsmentioning

confidence: 99%

The calcium-activated chloride channel-associated protein rCLCA2 is expressed throughout rat epidermis, facilitates apoptosis and is downmodulated by UVB

Hämäläinen

Bart

Takabe

et al. 2021

Histochem Cell Biol

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“…Other significantly increased the percutaneous penetration of the acid for a sufficient treatment time, that is, 5 min (Jung & Zhu, 2016).…”

Section: Discussionmentioning

confidence: 99%

A blinded study assessment of the efficacy of an original formula with retinol in combination with sonophoresis

Zasada

Drożdż

Erkiert‐Polguj

et al. 2019

Dermatologic Therapy

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An appropriately designed cosmetic product and regimen of treatment can improve facial skin appearance and provide good tolerability. The aim of the study was to evaluate the effect of 0.3 and 0.5% retinol in liquid crystal formula, applied with sonophoresis, on mature skin. Serum with various concentrations of retinol was applied to the left and right sides of the face and treated with ultrasound. The treatments were performed once a week for a period of 7 weeks. The skin condition before and after treatment was compared using the Fotomedicus system. The Visual Analogue Scale method enabled the results to be assessed by three independent specialists. The MultiProbe Adapter system was used to evaluate the efficacy of a series of treatments. Subjective assessment of volunteers identified perceived improvement of skin conditions. In addition, treatment was associated with greater skin moisture level, reduced wrinkling and decreased skin discoloration. Mild side effects appeared mainly after the first treatment and on the left side of the face with 0.5% retinol. More significant results may be obtained by extending the treatment duration in longer-term studies. Nonetheless, the findings confirmed the effectiveness of the procedures.

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“…At low doses it acts as an antioxidant, protecting cells from the harmful effects of ROS, but at high (mmol L −1 ) doses it can be a pro‐oxidant, leading to apoptosis . The topical formulations protecting against ultraviolet (UV) radiation contain relatively high concentrations of vitamin C (15–20%), but the biologically effective dose may be considerably lower owing to the poor penetration of vitamin C through stratum corneum …”

Section: Discussionmentioning

confidence: 99%

Hyaluronan metabolism enhanced during epidermal differentiation is suppressed by vitamin C

et al. 2018

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Effect of ultrasound and heat on percutaneous absorption of l‐ascorbic acid: human in vitro studies on Franz cell and Petri dish systems

Cited by 10 publications

References 17 publications

The calcium-activated chloride channel-associated protein rCLCA2 is expressed throughout rat epidermis, facilitates apoptosis and is downmodulated by UVB

The calcium-activated chloride channel-associated protein rCLCA2 is expressed throughout rat epidermis, facilitates apoptosis and is downmodulated by UVB

A blinded study assessment of the efficacy of an original formula with retinol in combination with sonophoresis

Hyaluronan metabolism enhanced during epidermal differentiation is suppressed by vitamin C

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