Effect of Ultrasound on Fibrinogen in Human Plasma

“…Protein was determined by the biuret method [13] using bovine serum albumin as standard. Orthophosphate (P~) was determined by the method of Fisk and SubbaRow [14].…”

Section: Assaysmentioning

confidence: 99%

Utilization of short and long-chain polyphosphates as energy sources for the anaerobic growth of bacteria

Varma¹

1983

FEMS Microbiology Letters

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“…It is noted that in some reports where the abnormal clotting of platelet fibrinogen has been described, surface-active substances (8) or ultrasonication (6, 7) has been used in the preparation of platelet extracts. Ultrasonication has been shown to exert a denaturing effect on fibrinogen in plasma (32) .…”

Section: -mentioning

confidence: 99%

Bovine Platelet Proteins

Łopaciuk¹,

No²

1969

Thromb Haemost

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Summary1. The protein composition of bovine platelet extracts has been investigated by immunoelectrophoresis and polyacrylamide disc electrophoresis. The information obtained is discussed as a basis for study on platelet fibrinogen.2. With antiserum to platelet proteins 11 precipitin lines were observed 3 of which corresponded electrophoretically to plasma albumin, fibrinogen and γ-globulin. These lines were not seen using the same antiserum absorbed with bovine plasma. The 8 additional lines were still present indicating that they represented specific platelet components. Antiserum to plasma produced the 3 above-mentioned lines, but no others.3. With antiserum to purified bovine plasma fibrinogen 3 precipitin lines were observed. The fibrinogen line was the dominant one. The 2 additional lines did not disappear by absorption of the antiserum with bovine serum nor by incubation of the extracts with thrombin. The latter treatment totally removed the fibrinogen line.4. A non-fibrinogen precipitin line, observed only with the antiserum to platelet extract and positioned in the β2-globulin region, disappeared by the incubation of platelet extracts with thrombin.

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Effect of Ultrasound on Fibrinogen in Human Plasma

Cited by 7 publications

References 3 publications

A reappraisal of the role of the low potential c-type cytochrome (cytochrome c-552) in NADH-dependent nitrite reduction and its relationship with a co-purified NADH oxidase in Escherichia coli K-12

A reappraisal of the role of the low potential c-type cytochrome (cytochrome c-552) in NADH-dependent nitrite reduction and its relationship with a co-purified NADH oxidase in Escherichia coli K-12

Utilization of short and long-chain polyphosphates as energy sources for the anaerobic growth of bacteria

Bovine Platelet Proteins

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