This study was used 100 laying hens, for the period from 10/26/2021 till 3/5/2022 to studying the effect of LEI0258 marker on some productive traits of Iraqi local chickens, Productive traits were measured from the sexual maturity up to 100 days for each chicken, the blood samples were collected from 100 laying hens at the age of 38 weeks from a brachial vein. The LEI0258 marker have 11 alleles were (A1, A2, A3, A4, B1, B2, B3, C1, C2, C3, D1). The results showed that there were a high significant differences (P ≤0.01) for (A1, B1) alleles compared with different alleles of percentage number and genetic distribution with a percentage of (18 %). there was a significant effect (P ≤0.05) in age at sexual maturity for different alleles, while in weight at sexual maturity there were non-significant difference between different alleles. Egg weight trait showed that there was a significant difference (P ≤ 0.05) for the periods (14, 42, 70, 100) day, and non-significant differences in the productive periods (28, 56, 84, 98) day. Feed intake ratio recorded non-significant differences in the period (14, 28, 42, 98, 100) day, while it recorded a significant difference (P ≤0.05) in the productive periods (56, 70, 84) day between different alleles. The qualitative traits revealed a significant difference (P ≤ 0.05) for (shell weight, yolk weight, yolk height, yolk diameter, egg white weight, egg diameter, egg white height, and HU unit), While non-significant differences in shell thickness between the different alleles.