Effect of UVA radiation on the Nrf2 signalling pathway in human skin cells

Ryšavá, Alena; Čížková, Kateřina; Franková, Jana; Roubalová, Lenka; Ulrichová, Jitka; Vostalová, Jitka; Vrba, Jiřı́; Zálešák, Bohumil; Svobodová, Alena Rajnochová

doi:10.1016/j.jphotobiol.2020.111948

Cited by 31 publications

(28 citation statements)

References 51 publications

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“…A similar direction of systemic redox state changes was also observed earlier in the literature [ 17 ]. Oxidizing conditions may affect the redox-sensitive transcription factor Nrf2, the expression and function of which may be impaired, as shown in previous in vitro studies in UV-irradiated skin cells [ 24 , 59 ]. However, these changes in expression are not supported by the results of this study on PMN fractions after UVA/UVB irradiation of the animal skin, but it is believed that not only changes in the biological activity of Nrf2 are important.…”

Section: Discussionmentioning

confidence: 99%

Oxidative Stress and Its Consequences in the Blood of Rats Irradiated with UV: Protective Effect of Cannabidiol

et al. 2021

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UVA/UVB radiation disturbs the redox balance of skin cells, and metabolic consequences can be transferred into the blood and internal tissues, especially after chronic skin exposure to UV radiation. Therefore, the aim of this study was to evaluate the effect of cannabidiol (CBD), an antioxidant and anti-inflammatory phytocannabinoid, on oxidative stress and its consequences in the blood of nude rats whose skin was exposed to UVA/UVB radiation for 4 weeks. It was shown that CBD penetrated the blood and in UVB-irradiated rats was preferentially located in the membranes of polymorphonuclear leukocytes, which promoted reduction of ROS generation and up-regulation of antioxidant ability by increasing the activity of glutathione reductase and thioredoxin reductase, while the level of reduced glutathione decreased by UV radiation. Consequently, reduction in UV-induced lipid peroxidation, assessed as 4-hydroxynonenal (4-HNE) and 8-isoprostane (8-isoPGF2α) as well as protein modifications, estimated as 4-HNE-protein adducts and protein carbonyl groups, was observed. CBD, by countering the UV-induced down-regulation of 2-arachidonylglycerol, promoted its antioxidant/anti-inflammatory effects by reducing CB1 and increasing PPARγ receptor activation and consequently ROS and TNF-α down-regulation. The results suggest that CBD applied topically to the skin minimizes redox changes not only at the skin level, but also at the systemic level.

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Section: Discussionmentioning

confidence: 99%

Oxidative Stress and Its Consequences in the Blood of Rats Irradiated with UV: Protective Effect of Cannabidiol

et al. 2021

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“…The cellular antioxidant mechanisms against ROS were shown to be upregulated following UVA irradiation. Rysava et al [12] showed that skin cells exhibit increasing Nrf2 activation levels, which is the main regulator of cellular antioxidant mechanisms against UV irradiation, peaking around 6 h following UVA exposure. However, after the peak, Nrf2 levels were shown to gradually decrease to significantly lower levels than that of non-irradiated cells.…”

Section: Discussionmentioning

confidence: 99%

“…Nuclear factor E2-related factor 2 (Nrf2), the redox-sensitive transcription factor, is a master regulator of the cellular antioxidant defense against environmental damage, including UV exposure [11]. Nrf2 has been reported to play a beneficial role in protecting skin cells, including keratinocytes, fibroblasts, and melanocytes, against UV-induced oxidative damage and cellular dysfunction [12]. Thus, investigation of compounds targeting Nrf2-regulated antioxidant defense to combat oxidative stress and targeting collagen formation mechanism to replenish digested collagen deposits could provide insight into development of a promising pharmacological approach to help delay skin photoaging.…”

Section: Introductionmentioning

confidence: 99%

Santamarine Shows Anti-Photoaging Properties via Inhibition of MAPK/AP-1 and Stimulation of TGF-β/Smad Signaling in UVA-Irradiated HDFs

Kim

Karadeniz

et al. 2021

Molecules

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Chronic UVA exposure results in elevated reactive oxygen species in skin which leads to photoaging characterized as upregulated matrix metalloproteinase (MMP)-1 and loss of collagen. Therefore, natural antioxidants are hailed as promising agents to be utilized against photoaging. In the current study, reynosin and santamarine, two known sesquiterpene lactones isolated from Artemisia scoparia, were analyzed for their anti-photoaging properties in UVA-irradiated human dermal fibroblasts (HDFs). Results showed that UVA irradiation (8 J/cm2) upregulated the MMP-1 secretion and expression, and suppressed collagen production, which were significantly reverted by santamarine treatment (10 µM). Although both reynosin and santamarine exhibited ROS scavenging abilities, reynosin failed to significantly diminish UVA-stimulated MMP-1 release. UVA-irradiated HDFs showed increased collagen production when treated with santamarine. As a mechanism to suppress MMP-1, santamarine significantly suppressed the UVA-induced phosphorylation of p38 and JNK and nuclear translocation of p-c-Fos and p-c-Jun. Santamarine promoted collagen I production via relieving the UVA-induced suppression on TGF-β and its downstream activator Smad2/3 complex. Antioxidant properties of santamarine were also shown to arise from stimulating Nrf2-dependent expression of antioxidant enzymes SOD-1 and HO-1 in UVA-irradiated HDFs. In conclusion, santamarine was found to be a promising natural antioxidant with anti-photoaging properties against UVA-induced damages in HDFs.

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“…Factors which influence Nrf2 expression are displayed in blue, while downstream implications of Nrf2 expression are shown in orange. Lists below bubbles display specific compounds involved, with red font representing inhibition and black font indicating activation ( Kensler et al, 2007 ; Merry and Ristow, 2016 ; Itoh et al, 2010 ; Domazetovic et al, 2017 ; Huang et al, 2015 ; Alam et al, 1999 ; Thimmulappa et al, 2002 ; Chen and Maltagliati, 2017 ; Ajiboye et al, 2014 ; Zhou et al, 2014 ; Yamaguchi et al, 2018 ; Liu et al, 2018 ; Florczyk et al, 2014 ; Toledo-Arruda et al, 2020 ; Zhou et al, 2020 ; Cheng et al, 2016 ; Tsukimoto et al, 2010 ; Liu et al, 2019b ; Rysava et al, 2020 ; Wang et al, 2020b ; McDonald et al, 2010 ; Bellanti et al, 2013 ; Zimta et al, 2019 ). …”

Section: The Keap1-nrf2 Pathwaymentioning

confidence: 99%

The role of the Nrf2/Keap1 signaling cascade in mechanobiology and bone health

Priddy

2021

Bone Reports

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Effect of UVA radiation on the Nrf2 signalling pathway in human skin cells

Cited by 31 publications

References 51 publications

Oxidative Stress and Its Consequences in the Blood of Rats Irradiated with UV: Protective Effect of Cannabidiol

Oxidative Stress and Its Consequences in the Blood of Rats Irradiated with UV: Protective Effect of Cannabidiol

Santamarine Shows Anti-Photoaging Properties via Inhibition of MAPK/AP-1 and Stimulation of TGF-β/Smad Signaling in UVA-Irradiated HDFs

The role of the Nrf2/Keap1 signaling cascade in mechanobiology and bone health

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