Effect of Vaccination on<i>Bordetella pertussis</i>Strains, China

Zhang, Liu; Xu, Yinghua; Zhao, Jianhong; Kallonen, Teemu; Cui, Shenghui; Xu, Yunqiang; Hou, Qing; Li, Fengxiang; Wang, Junzhi; He, Qiushui; Zhang, Shumin

doi:10.3201/eid1611.100401

Cited by 29 publications

(29 citation statements)

References 30 publications

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“…Allele frequencies of the virulence-associated allelic variants, fim3B , ptxA1 , prn2, and ptxP3 , have increased within the B. pertussis population in several countries [10], [12]–[17]. B. pertussis strains carrying ptxP3 are more capable of producing pertussis toxin (PT) than are ptxP1 strains, and the emergence of ptxP3 strains was associated with pertussis resurgence in the Netherlands [18].…”

Section: Introductionmentioning

confidence: 99%

Genetic Analysis of Bordetella pertussis Isolates from the 2008–2010 Pertussis Epidemic in Japan

et al. 2013

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A large pertussis epidemic occurred between 2008 and 2010 in Japan. To investigate epidemic strains, we analyzed 33 Bordetella pertussis isolates from the epidemic period by sequencing virulence-associated genes (fim3, ptxP, ptxA, and prn) and performing multilocus variable-number tandem repeat analysis (MLVA), and compared these results with those of 101 isolates from non-epidemic, earlier and later time periods. DNA sequencing of the fim3 allele revealed that the frequency of fim3B was 4.3%, 12.8%, 30.3%, and 5.1% within isolates in 2002–2004, 2005–2007, 2008–2010, and 2011–2012, respectively. The isolation rate of the fim3B strain therefore temporarily increased during the epidemic period 2008–2010. In contrast, the frequencies of the virulence-associated allelic variants, ptxP3, ptxA1, and prn2, increased with time during overall study period, indicating that these variants were not directly involved in the occurrence of the 2008–2010 epidemic. MLVA genotyping in combination with analysis of allele types showed that the prevalence of an MT27d strain temporarily increased in the epidemic period, and that this strain carried virulence-associated allelic variants (fim3B, ptxP3, ptxA1, and prn2) also identified in recent epidemic strains of Australia, Europe, and the US. Phenotypic analyses revealed that the serotype Fim3 strain was predominant (≥87%) during all the periods studied, and that the frequency of adhesion pertactin (Prn) non-expressing B. pertussis decreased by half in the epidemic period. All MT27d strains expressed Prn and Fim3 proteins, suggesting that B. pertussis MT27d strains expressing Prn and Fim3B have the potential to cause large epidemics worldwide.

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Section: Introductionmentioning

confidence: 99%

Genetic Analysis of Bordetella pertussis Isolates from the 2008–2010 Pertussis Epidemic in Japan

et al. 2013

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“…All four isolates harbored the allele combination ptxA1 ptxP1, as determined by PCR with the primers and protocol recommended for genotyping (4). The allele combination observed is common in modern Chinese isolates (5). Of the four isolates, three had the pulsed-field gel electrophoresis (PFGE) profile BpFINR9 and one had the BpSR23 profile; they belonged to the same cluster (cluster III) (6).…”

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confidence: 99%

Appearance of Macrolide-Resistant Bordetella pertussis Strains in China

Wang

Hou

et al. 2013

Antimicrob Agents Chemother

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“…Comparative genetic analysis of the genes for B. pertussis PT and pertactin (an outer-membrane protein) between epidemic isolates and the vaccine strains revealed that expansion of strains antigenically distinct from vaccine strains has occurred [340,341,[348][349][350][352][353][354]. These findings strongly implicate vaccination as a strong driving force in the continuous evolution of the B. pertussis population and may forebode the emergence of novel variants resistant to vaccination.…”

Section: Vaccines and Toxin Evolutionmentioning

confidence: 90%

“…One possible explanation for this reemergence is that the constant selective pressure imposed by immunization might have resulted in increased antigenic divergence in the remaining bacterial population. Consequently, the effects of vaccination on toxin evolution are beginning to be examined [340,341,343,[348][349][350][351][352][353].…”

Section: Vaccines and Toxin Evolutionmentioning

confidence: 99%