Effect of vaginal probiotic lactobacilli on in vitro–induced sperm lipid peroxidation and its impact on sperm motility and viability

Barbonetti, Arcangelo; Cinque, Benedetta; Vassallo, M. R. C.; Mineo, Salvatore; Francavilla, Sandro; Cifone, Maria Grazia; Francavilla, Felice

doi:10.1016/j.fertnstert.2011.03.066

Cited by 64 publications

(57 citation statements)

References 25 publications

(31 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As their proportion in semen was remarkably high according to the present study, as well as some other studies, a similar function might be attributed to them in the male genital tract . Semen quality in Lactobacillus ‐predominant semen samples is higher than in the case of the other community types, and lactobacilli prevent sperm lipid peroxidation, thus preserving sperm motility and viability …”

Section: Discussionsupporting

confidence: 86%

Seminal microbiome in men with and without prostatitis

et al. 2017

View full text Add to dashboard Cite

Objectives: To profile the seminal microbiome applying next generation sequencing. Methods: Semen samples of 67 men were involved in the study (21 men with and 46 men without prostatitis). Seminal microbiomes were profiled applying the method that uses combinatorial sequence tags attached to polymerase chain reaction primers that amplify the ribosomal ribonucleic acid V6 region. Amplified polymerase chain reaction products were sequenced using an Illumina paired-end protocol on HiSeq2000 platform. Results: The most abundant phylum in semen was Firmicutes, comprising nearly half of the sequences found (median 41.7%, quartiles 28.5-47.2%) followed by Bacteroidetes, Proteobacteria and Actinobacteria. The counts of lactobacilli were higher in healthy men than prostatitis patients (27% [20.2-34.6%] vs 20.2% [4.9-25.0%]; P = 0.05), especially for Lactobacillus iners. Proteobacteria comprised higher proportions in prostatitis patients than healthy men. The species richness was higher in prostatitis patients than healthy men (inverted Simpson index 13.5 AE 5.8 vs 10.3 AE 4.0). Conclusions: The semen of chronic prostatitis patients contains fewer healthsupporting lactobacilli, and has higher species diversity than that of healthy men. Firmicutes (especially lactobacilli), Bacteroidetes, Proteobacteria and Actinobacteria comprise the highest proportion of seminal microbiome.

show abstract

Section: Discussionsupporting

confidence: 86%

Seminal microbiome in men with and without prostatitis

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Indeed, lactobacilli exhibit antioxidant properties, which provide their protection against ROS attack when they colonize the host mucosal surfaces [38-40]. Accordingly, we have recently reported that the same mix of three selected strains of lactobacilli, used in the present study, at the same CFU concentration, also prevented sperm lipid peroxidation induced in vitro by a ferrous ion promoter, thus preserving sperm motility and viability [22]. Intriguingly, it has been recently demonstrated that L. plantarum produces carotenoids [40], exerting well known antioxidant actions within biological membranes [41].…”

Section: Discussionmentioning

confidence: 86%

“…Sperm motility was evaluated by Computer-Aided Semen Analysis (CASA) using ATS20 (JCD, Gauville, France), as previously described [15,17,22]. At least 200 spermatozoa were examined for each sample using standard settings: 30 frames acquired at a frame rate of 60 Hz and a temperature of 37°C in 20-μm deep chambers.…”

Section: Methodsmentioning

confidence: 99%

“…Lipid peroxidation was evaluated using the probe BODIPY 581/591 C 11 , which is incorporated into sperm membranes and responds to free radical attack with a spectral emission shift from red to green [17,22,24]. Albumin-free BWW medium was used because it was found that bovine serum albumin (BSA) binds the lipophilic BODIPY C 11 .…”

Section: Methodsmentioning

confidence: 99%

“…Intriguingly, lactobacilli could exert anti-oxidant protective effects on spermatozoa. In fact, as we recently demonstrated, soluble factors produced by a mix of 3 selected strains of lactobacilli ( L. brevis CD2, L. salivarius FV2, and L. plantarum FV9), which are effective in treating bacterial vaginosis in the form of vaginal tablets [21], prevented lipid peroxidation of sperm membrane induced in vitro by ferrous ion, thus preserving sperm motility and vitality [22]. …”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Soluble Products of Escherichia coli Induce Mitochondrial Dysfunction-Related Sperm Membrane Lipid Peroxidation Which Is Prevented by Lactobacilli

et al. 2013

Self Cite

View full text Add to dashboard Cite

Unidentified soluble factors secreted by E. coli, a frequently isolated microorganism in genitourinary infections, have been reported to inhibit mitochondrial membrane potential (ΔΨm), motility and vitality of human spermatozoa. Here we explore the mechanisms involved in the adverse impact of E. coli on sperm motility, focusing mainly on sperm mitochondrial function and possible membrane damage induced by mitochondrial-generated reactive oxygen species (ROS). Furthermore, as lactobacilli, which dominate the vaginal ecosystem of healthy women, have been shown to exert anti-oxidant protective effects on spermatozoa, we also evaluated whether soluble products from these microorganisms could protect spermatozoa against the effects of E. coli. We assessed motility (by computer-aided semen analysis), ΔΨm (with JC-1 dye by flow cytometry), mitochondrial ROS generation (with MitoSOX red dye by flow cytometry) and membrane lipid-peroxidation (with the fluorophore BODIPY C11 by flow cytometry) of sperm suspensions exposed to E. coli in the presence and in the absence of a combination of 3 selected strains of lactobacilli (L. brevis, L. salivarius, L. plantarum). A Transwell system was used to avoid direct contact between spermatozoa and microorganisms. Soluble products of E. coli induced ΔΨm loss, mitochondrial generation of ROS and membrane lipid-peroxidation, resulting in motility loss. Soluble factors of lactobacilli prevented membrane lipid-peroxidation of E. coli-exposed spermatozoa, thus preserving their motility. In conclusion, sperm motility loss by soluble products of E. coli reflects a mitochondrial dysfunction-related membrane lipid-peroxidation. Lactobacilli could protect spermatozoa in the presence of vaginal disorders, by preventing ROS-induced membrane damage.

show abstract

A systematic and comprehensive review of the role of microbiota in urinary chronic pelvic pain syndrome

Hashemi,

Tondro Anamag,

Javan Balegh Marand

et al. 2024

Neurourology and Urodynamics

View full text Add to dashboard Cite

BackgroundMany genitourinary tract disorders could be attributed partly to the microbiota. This study sought to conduct a systematic review of the role of the microbiota in urinary chronic pelvic pain syndrome (UCPPS).MethodsWe searched Embase, Scopus, Web of Science, and PubMed with no time, language, or study type restrictions until December 1, 2023. The JBI Appraisal Tool was used to assess the quality of the studies. Study selection followed the PRISMA statement. Studies addressing microbiome variations among patients suffering from interstitial cystitis/bladder pain syndrome (IC/BPS) or chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) and a control group were considered eligible.ResultsA total of 21 studies (1 UCPPS, 12 IC/BPS, and 8 CP/CPPS) comprising 1125 patients were enrolled in our final data synthesis. It has been shown that the reduced diversity and discrepant composition of the gut microbiota may partly be attributed to the UCPPS pathogenesis. In terms of urine microbiota, some operational taxonomic units were shown to be elevated, while others became less abundant. Furthermore, various bacteria and fungi are linked to specific clinical features. Few investigations denied UCPPS as a dysbiotic condition.ConclusionsUrinary and intestinal microbiota appear to be linked with UCPPS, comprising IC/BPS and CP/CPPS. However, given the substantial disparity of published studies, a battery of prospective trials is required to corroborate these findings.

show abstract

Effect of vaginal probiotic lactobacilli on in vitro–induced sperm lipid peroxidation and its impact on sperm motility and viability

Cited by 64 publications

References 25 publications

Seminal microbiome in men with and without prostatitis

Seminal microbiome in men with and without prostatitis

Soluble Products of Escherichia coli Induce Mitochondrial Dysfunction-Related Sperm Membrane Lipid Peroxidation Which Is Prevented by Lactobacilli

A systematic and comprehensive review of the role of microbiota in urinary chronic pelvic pain syndrome

Contact Info

Product

Resources

About