Effect of various blending ratios on the cell characteristics of PCL and PLGA scaffolds fabricated by polymer deposition system

Sa, Min-Woo; Kim, Jong‐Young

doi:10.1007/s12541-013-0087-x

Cited by 28 publications

(15 citation statements)

References 18 publications

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“…The scaffold usually regulates the growth of cells that either shift from neighboring tissue or arise inside the porous structure of the scaffold. Synthetic scaffolds are semi‐crystalline materials, and owing to their cost‐efficiency, high toughness, and biocompatibility, they are one of the most extensively used biodegradable polymers [–]. The gross design, microstructure, material composition, and mechanical property play a major role in controlling the local environment and growth of the adhered cells on the scaffolds [–].…”

Section: Introductionmentioning

confidence: 99%

Unraveling the mechanical strength of biomaterials used as a bone scaffold in oral and maxillofacial defects

Prasadh

Wong

2018

Oral Science International

233

125

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Three‐dimensional printed natural and synthetic biomaterials have evolved as gold standards for tissue engineering scaffolds in recent trends owing to their superior role in hard tissue regeneration. The major drawback of these scaffolds is their relatively poor mechanical strength. Another key consideration in the design of the scaffolds is the difficulty in replicating the complex structural composition of hard tissues such as bones, and its structure cannot be reproduced with a single material that provides a limited range of properties. Sufficient mechanical strength is provided by the structure required for the replacement tissue. The mechanical properties of the scaffold play an important role in many applications of tissue engineering. Therefore, is it sufficient to withstand the force with only a single material used for the scaffold? There are many materials such as natural resin, synthetic resin, and polymers. They are used in combination to fulfill the function and to act as a kingpin by solving their drawbacks. The added material is not only superior in mechanical strength but also compatible with the tissues surrounding the implant, promoting cell adhesion and gradually degrading rather than intoxicating the patient. This review focuses on the various biomaterials used as scaffolds for critical size defects and the aftermath in their mechanical properties.

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Section: Introductionmentioning

confidence: 99%

Unraveling the mechanical strength of biomaterials used as a bone scaffold in oral and maxillofacial defects

Prasadh

Wong

2018

Oral Science International

233

125

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“…Following cell attachment and proliferation, the material should break down and be replaced by the extracellular matrix ( 32 , 33 ). A previous study indicated that hybrid PCL/PLGA may facilitate the adhesion and proliferation of stem cells ( 34 ). The present data demonstrated that hPDLSCs on the 0.5PCL/0.5PLGA and 0.1PCL/0.9PLGA scaffolds had a significantly higher rate of attachment and proliferation compared with the 0.9PCL/0.1PLGA scaffolds.…”

Section: Discussionmentioning

confidence: 99%

Response of hPDLSCs on 3D printed PCL/PLGA composite scaffolds in�vitro

et al. 2018

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Three-dimensional printed (3DP) scaffolds have become an excellent resource in alveolar bone regeneration. However, selecting suitable printable materials remains a challenge. In the present study, 3DP scaffolds were fabricated using three different ratios of poly (ε-caprolactone) (PCL) and poly-lactic-co-glycolic acid (PLGA), which were 0.1PCL/0.9PLGA, 0.5PCL/0.5PLGA and 0.9PCL/0.1PLGA. The surface characteristics and degradative properties of the scaffolds, and the response of human periodontal ligament stem cells (hPDLSCs) on the scaffolds, were assessed to examine the preferable ratio of PCL and PLGA for alveolar bone regeneration. The results demonstrated that the increased proportion of PLGA markedly accelerated the degradation, smoothed the surface and increased the wettability of the hybrid scaffold. Furthermore, the flow cytometry and Cell Counting Kit-8 assay revealed that the adhesion and proliferation of hPDLSCs were markedlyincreased on the 0.5PCL/0.5PLGA and 0.1PCL/0.9PLGA scaffolds. Additionally, the alkaline phosphatase activity detection and reverse-transcription quantitative polymerase chain reaction demonstrated that the hPDLSCs on the 0.5PCL/0.5PLGA scaffold exhibited the best osteogenic capacity. Consequently, PCL/PLGA composite scaffolds may represent a candidate focus for future bone regeneration studies, and the 0.5PCL/0.5PLGA scaffold demonstrated the best bio-response from the hPDLSCs.

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“…The CsA-Algi gel can also be utilized as a carrier for cell transplantation. The CsA-loaded 3D drug carrier, which is composed of the CsA-Algi gel and the PCL/PLGA polymeric framework, can endure a compressive force up to several 10s of MPa (23,31,36,39,40,47), and can thus resist breaking and unintentional fast drug release. Many researchers have investigated the improvement of the mechanical properties of hydrogels.…”

Section: Discussionmentioning

confidence: 99%

3D-Printed Drug/Cell Carrier Enabling Effective Release of Cyclosporin a for Xenogeneic Cell-Based Therapy

et al. 2015

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Systemic administration of the immunosuppressive drug cyclosporin A (CsA) is frequently associated with a number of side effects; therefore, sometimes it cannot be applied in sufficient dosage after allogeneic or xenogeneic cell transplantation. Local delivery is a possible solution to this problem. We used 3D printing to develop a CsA-loaded 3D drug carrier for the purpose of local and sustained delivery of CsA. The carrier is a hybrid of CsA-poly(lactic-co-glycolic acid) (PLGA) microsphere-loaded hydrogel and a polymeric framework so that external force can be endured under physiological conditions. The expression of cytokines, which are secreted by spleen cells activated by Con A, and which are related to immune rejection, was significantly decreased in vitro by the released CsA from the drug carrier. Drug carriers seeded with xenogeneic cells (human lung fibroblast) were subcutaneously implanted into the BALB/c mouse. As a result, T-cell-mediated rejection was also significantly suppressed for 4 weeks. These results show that the developed 3D drug carrier can be used as an effective xenogeneic cell delivery system with controllable immunosuppressive drugs for cell-based therapy.

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Effect of various blending ratios on the cell characteristics of PCL and PLGA scaffolds fabricated by polymer deposition system

Cited by 28 publications

References 18 publications

Unraveling the mechanical strength of biomaterials used as a bone scaffold in oral and maxillofacial defects

Unraveling the mechanical strength of biomaterials used as a bone scaffold in oral and maxillofacial defects

Response of hPDLSCs on 3D printed PCL/PLGA composite scaffolds in�vitro

3D-Printed Drug/Cell Carrier Enabling Effective Release of Cyclosporin a for Xenogeneic Cell-Based Therapy

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