Effect of various extenders and taurine on survival of stallion sperm cooled to 5 °C

Ahmad, Irfan; Ducharme, R.

doi:10.1016/0093-691x(95)00290-o

Cited by 35 publications

(23 citation statements)

References 25 publications

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“…Adding antioxidants to the extender solution is an effective method to protect boar semen quality during preservation (Funahashi & Sano 2005). As an antioxidant, taurine is present naturally in semen at low levels (319-1590 lmol/L in human seminal fluid) (Meizel et al 1980;Holmes et al1992) and could protect the spermatozoa of many animals (Ijaz & Ducharme 1995;Atessahin et al 2008). Sperm motility as an important parameter, for sperm conservation correlates with preservation time, farrowing rate and litter size reduction (Holt et al 1997).…”

Section: Discussionmentioning

confidence: 99%

“…According to previous studies, taurine mainly suppresses oxidation in two main ways: as a direct antioxidant and by increasing the activity of antioxidant enzymes. Taurine could increase the activity and levels of antioxidant enzymes to decrease ROS and reduce superoxide production in spermatozoa, and concomitantly reduce the inactivation of superoxide dismutase (SOD) in cells (Ijaz & Ducharme 1995;Yu & Kim 2009). By contrast, seminal plasma antioxidant systems contain a variety of antioxidant enzymes that counteract the destructive effects of excessive ROS (Garrido et al 2004).…”

Section: Figurementioning

confidence: 99%

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Supplemental effect of different levels of taurine in Modena on boar semen quality during liquid preservation at 17°C

Zhang

Qian

et al. 2017

Animal Science Journal

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Peroxidation damage induces sublethal injury to boar sperm during the storage process. Taurine has already been demonstrated to protect cells effectively from oxidant-induced injury. This study was aimed to evaluate the effect of different concentrations of taurine (0.5, 1, 5 and 10 mmol/L) in Modena diluent on boar sperm quality during liquid storage at 17°C. Ejaculates from sexually mature Duroc pigs were collected, pooled and preserved in the Modena containing different concentrations of taurine. Sperm motility, plasma membrane integrity, acrosome integrity, total antioxidative capacity (T-AOC) activity and malondialdehyde content (MDA) were examined every 24 h. Modena diluent containing taurine suppressed the reduction in sperm qualities during the process of liquid preservation compared with those of the control group. After 5 days of liquid preservation, the addition of taurine at 5 mmol/L had the optimal effect on survival time as well as maintenance of motility, plasma membrane integrity, acrosomal integrity, T-AOC activity and MDA content. These results may suggest the possibility that the proper addition of taurine to the semen extender improves the swine production system using artificial insemination by the suppressing of sperm damage and subsequent dysfunction during liquid preservation.

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Section: Discussionmentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

Supplemental effect of different levels of taurine in Modena on boar semen quality during liquid preservation at 17°C

Zhang

Qian

et al. 2017

Animal Science Journal

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“…Como em outras espécies de produção, o sucesso da inseminação artificial nos equinos depende da manutenção da viabilidade e fertilidade do sêmen durante o armazenamento (Ijaz& Ducharme, 1995). A refrigeração, estocagem e transporte do sêmen para uso em programas de inseminação artificial são de vital importância para o manejo reprodutivo de diversas espécies (Miró et al, 2009).…”

Section: Métodos De Preservação Do Sêmen Equino Refrigeradounclassified

Problemas relacionados ao uso de sêmen refrigerado de garanhões

2016

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ResumoInúmeras biotecnologias se tornaram difundidas, sendo a inseminação artificial a mais utilizada na indústria equina. Pela necessidade em aumentar a longevidade dos espermatozoides e garantir altas taxas de fertilização, a tecnologia de refrigeraçãoespermática tem sido amplamente estudada e utilizada comercialmente na produção de cavalos.No entanto, até o presente existem alguns pontos que impedem a difusão plena dessa biotecnologia, como os resultados inconstantes e as vezes insatisfatórios que ocorrem ao trabalhar com sêmen refrigerado. O objetivo deste trabalho foi revisar os pontos críticos relativos à utilização de sêmen refrigerado equino em programas de reprodução animal na espécie.Palavras-Chave: Sêmen. Eqüino. Refrigeração. Gustavo Mendes Gomes¹, André Crespilho¹, ², Letícia M. Gomes¹. Relating problem to cooled semen of stallion Problemas relacionados ao uso de sêmen refrigerado de garanhões AbstractSeveral biotechnologies become widespread and artificial insemination is the most used on horses. Aiming the increasein the sperm longevity with acceptable fertilization rates, sperm cooling technologyhas been widelystudied andused commerciallyin the horse production.However, there are some critical points that hinder the diffusion of the biotechnology such as unsatisfying results when using cooled semen. The aim of this article was to review the critical points relating to the utilization of cooled stallion semen in equine breeding programs.

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“…Extenders used in this study were skim milk (SM) based extender (Kenney et al, 1975) and Dimitropoulos extender (Ijaz & Durcarme, 1999). SM was composed of 2.4 g SM and 4 g glucose, diluted in 100 mL water.…”

Section: Extendersmentioning

confidence: 99%

The Quality of Stallion Semen in Skim Milk and Dimitropoulos Extenders Preserved at 5 oC and Ambient Temperature Supplemented with Different Sugar

Arifiantini¹,

Purwantara²,

Yusuf³

et al. 2013

med pet

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This study was conducted to evaluate the effects of sugars supplementation in skim milk based (SM) and dimitropoulos (DV) extenders on the sperm motility and viability in stallion semen storage at 5 o C and ambient temperature (24-29 o C). Semen samples were collected from 3 stallions; evaluate individually and each of them divided into 8 aliquots. Four out of eight aliquots were diluted 1:1 with SM, while the remaining four were diluted 1:1 with DV; all were then centrifuged at 1006 g (3000 RPM) for 15 min. The supernatants were discarded, and each pellet was re-diluted with SM (control), SM trehalose (SMT), SM-raffinose (SMR), SM-fructose (SMF), DV (control), DV-trehalose (DVT), DV-raffinose (DVR), and DV-fructose (DVF). The diluted semen were divided into 2 aliquots and stored at 5 o C or ambient temperature. The sperm motility and viability were evaluated every 3 h on chilled semen stored at ambient temperature, and every 12 h on those stored at 5 o C. Results of the experiments demonstrated that sperm motility and viability in DV extender significantly higher (P<0.05) in both temperature. The supplementation of fructose was the best on the motility and viability of the sperm at both temperatures compare to trehalose, raffinose, or the control group. The best extender and sugar combination was DVF, which the total motile sperm stored at 5 o C for 96 h was 45.1% followed by to DVT (40.2%) and DVR (39.2%). The sperm motility in DV and SMF were 35.3% and 35.6%, respectively; these were higher than those diluted with control (28.9%); SMT (30.3%), and SMR (29.6%). The study concluded that the supplementation of fructose in DV extender (DVF) was the best combination to preserve stallion sperm motility and viability stored at 5 o C or ambient temperature.

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Effect of various extenders and taurine on survival of stallion sperm cooled to 5 °C

Cited by 35 publications

References 25 publications

Supplemental effect of different levels of taurine in Modena on boar semen quality during liquid preservation at 17°C

Supplemental effect of different levels of taurine in Modena on boar semen quality during liquid preservation at 17°C

Problemas relacionados ao uso de sêmen refrigerado de garanhões

The Quality of Stallion Semen in Skim Milk and Dimitropoulos Extenders Preserved at 5 oC and Ambient Temperature Supplemented with Different Sugar

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