1997
DOI: 10.1002/(sici)1520-6866(1997)17:1<29::aid-tcm5>3.0.co;2-h
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Effect of various genotoxins and reproductive toxins in human lymphocytes and sperm in the Comet assay

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Cited by 75 publications
(33 citation statements)
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“…In vitro effect of genistein is under controversy due in part to differences in experimental conditions. A first series of studies developed with samples from only one donor [36][37][38] showed a negative effect of genistein (1-250 µmol L −1 ) on the DNA integrity of spermatozoa measured by comet assay in a similar response to H 2 O 2 , suggesting the hypothesis that genistein could generate ROS that target DNA [38]. However, other studies [15,17] showed that use of genistein at lower concentrations (0.01, 0.1, 1, 10 and 100 µmol L −1 ), produced a significant reduction in DNA damage after cells had been damaged by treatments with hydrogen peroxide.…”
Section: Discussionmentioning
confidence: 99%
“…In vitro effect of genistein is under controversy due in part to differences in experimental conditions. A first series of studies developed with samples from only one donor [36][37][38] showed a negative effect of genistein (1-250 µmol L −1 ) on the DNA integrity of spermatozoa measured by comet assay in a similar response to H 2 O 2 , suggesting the hypothesis that genistein could generate ROS that target DNA [38]. However, other studies [15,17] showed that use of genistein at lower concentrations (0.01, 0.1, 1, 10 and 100 µmol L −1 ), produced a significant reduction in DNA damage after cells had been damaged by treatments with hydrogen peroxide.…”
Section: Discussionmentioning
confidence: 99%
“…have undergone arcsine transformation and square root changing. (12)(13)(14)(15), ii) the single-cell gel electrophoresis (comet) assay (16)(17)(18), and iii) the in situ nick translation assay with or without sperm decondensation (19,20). Indirect methods include: i) the acridine orange technique (AOT), first introduced by Teyada et al (10), and ii) sperm chromatin structure assay (SCSA) (21).…”
Section: 00 --------------------------------------------------------mentioning
confidence: 99%
“…Cotelle and Ferard, 1999 [28] have demonstrated that the assay when applied to plants, worms, molluscs, fish, amphibians and mammals provides a sensitive and rapid system for the study of environmental genotoxicity. 4-Nonylphenol has been shown to be positive in the comet assay using human sperm and human peripheral lymphocytes in male and female donors [29].…”
Section: Discussionmentioning
confidence: 99%