Effect of Various Growth Conditions on Production of Extracellular Amylase from Thermotolerant Bacillus Species Isolated from Hot Springs in Jordan

Utong, James; Al-Quadan, Farouk; Akel, Hazem

doi:10.3923/jbs.2006.621.625

J. of Biological Sciences

2006

DOI: 10.3923/jbs.2006.621.625

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Effect of Various Growth Conditions on Production of Extracellular Amylase from Thermotolerant Bacillus Species Isolated from Hot Springs in Jordan

James Utong¹,

Farouk Al-Quadan²,

Hazem Akel³

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Cited by 6 publications

References 25 publications

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Characteristics of a Novel Highly Thermostable and Extremely Thermophilic Alkalitolerant Amylase from Hyperthermophilic Bacillus Strain HUTBS71

Al-Quadan¹,

Akel²,

Natshi³

2009

OnLine J. of Biological Sciences

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Problem statement:This study reported the purification and characterization of a novel highly thermostable alkaline amylase from a newly isolated Bacillus strain HUTBS71. Approach: The enzyme was purified using ammonium sulfate precipitation, ion exchange and gel filtration chromatography. Results: Maximum amylase activity (72 U mL −1 ) was obtained at 100°C after 10 min of incubation. The enzyme was purified 24 fold with 12.5% yield and showed a monomer band with a molecular weight of 58.8 kDa by SDS-PAGE. This enzyme exhibited maximum activity at pH and temperature, 7.8 and 100°C, respectively. It performed stability over a broad range of pH and temperature, 5.2-10.0 and 80-115°C, respectively. The half-life of the enzyme at 90 and 100°C was estimated to be 3 h. The activation energy of denaturation of purified enzyme was 2.53 kJ moL −1 . The enzyme was activated by 5 mM of CoCl 2 , MgSO 4 , MnCl 2 , ZnSO 4 and MnSO 4 (relative activity was 133, 126, 133, 106.6 and 103%, respectively). It was strongly inhibited by CuSO 4 and CdCl 2 but less affected by NaCl, CaCl 2 , FeCl 3 , ZnCl 2 and EDTA. Conclusion: The present purified amylase therefore could be defined as a highly thermostable, extremely hyperthermophilic and alkalitolerant with new properties make the present enzyme applicable for many starch processing and food industries.

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Characteristics of a Novel Highly Thermostable and Extremely Thermophilic Alkalitolerant Amylase from Hyperthermophilic Bacillus Strain HUTBS71

Al-Quadan¹,

Akel²,

Natshi³

2009

OnLine J. of Biological Sciences

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Effect of Metal Ions, EDTA and Sulfhydryl Reagents on Soybean Amylase Activity

Prakash¹,

Jaiswal²,

Pandey³

2011

Asian J. of Biochemistry

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Halotolerant Amylase Production by a Novel Bacterial Strain, Rheinheimera aquimaris

Ghasemi¹,

Rasoul-Ami²,

Ebrahimine³

et al. 2010

Research J. of Microbiology

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Effect of Various Growth Conditions on Production of Extracellular Amylase from Thermotolerant Bacillus Species Isolated from Hot Springs in Jordan

Cited by 6 publications

References 25 publications

Characteristics of a Novel Highly Thermostable and Extremely Thermophilic Alkalitolerant Amylase from Hyperthermophilic Bacillus Strain HUTBS71

Characteristics of a Novel Highly Thermostable and Extremely Thermophilic Alkalitolerant Amylase from Hyperthermophilic Bacillus Strain HUTBS71

Effect of Metal Ions, EDTA and Sulfhydryl Reagents on Soybean Amylase Activity

Halotolerant Amylase Production by a Novel Bacterial Strain, Rheinheimera aquimaris

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