Effect of various levels of dissolved oxygen on reactive oxygen species and cryocapacitation‐like changes in bull sperm

Amin, Beigh Yaqoob; Prasad, J.K.; Ghosh, Subrata; Lone, Shabir Ahmad; Kumar, Abhishek; Mustapha, Adam; Din, Omer; Kumar, Ajay

doi:10.1111/rda.13200

Cited by 23 publications

(25 citation statements)

References 39 publications

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“…Experts have developed media for sperm survival during the cooling and freezing techniques [15]. Many challenges, such as media toxicity [52], irregular pH [53], ROS [54], energy source [55], sperm membrane damage [24], and cryo-shock preservatives [56], were faced and have been resolved. Extenders protect sperm, conserve motility, and fertility over time by stabilizing the plasmalemma, provide energy substrates, and prevent harmful effects of pH and osmolarity changes [57].…”

Section: Development Of Semen Extendermentioning

confidence: 99%

Semen extenders: An evaluative overview of preservative mechanisms of semen and semen extenders

Bustani

Baiee

2021

Vet World

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Reproduction is fundamental for all living things as it ensures the continued existence of a species and an improved economy in animal husbandry. Reproduction has developed since history, and diverse processes, such as artificial insemination and in vitro fertilization, have been developed. Semen extenders were discovered and developed to protect sperm from harmful factors, such as freeze and osmotic shock, oxidative stress, and cell injury by ice crystals. Semen extenders preserve sperm by stabilizing its properties, including sperm morphology, motility, and viability and membrane, acrosomal, and DNA integrity. Therefore, semen extenders must provide a favorable pH, adenosine triphosphate, anti-cooling and anti-freeze shock, and antioxidant activity to improve semen quality for fertilization. Hence, this review provides precise data on different semen extenders, preservative mechanisms, and essential additives for semen extenders in different animals.

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Section: Development Of Semen Extendermentioning

confidence: 99%

Semen extenders: An evaluative overview of preservative mechanisms of semen and semen extenders

Bustani

Baiee

2021

Vet World

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“…Three different approaches have been used i.e. flash frozenthaw process using liquid nitrogen, nitrogen gas bubbling/ gassing process (2-3 bubbles per second) using gaseous nitrogen and applying negative pressure to the flask containing diluent with the help of modified vacuum pump (Pande et al, 2015;Mustapha et al, 2017;Amin et al, 2018;Balamurugan et al, 2018;Kumar et al, 2018). Further refinement of DO level was indicated in nitrogen gassing method as a gap between the 4 ppm and 8 ppm existed.…”

Section: Introductionmentioning

confidence: 99%

Partial Deoxygenation of Semen Extender Minimizes Post-thaw Damages and Improves Freezability of Crossbred Bull Spermatozoa

Bhutia¹,

Kumar²,

Katiyar³

et al. 2021

IJAR

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Background: Oxidative stress occurs when oxygen or oxygen derived oxidants exceed antioxidants and become responsible for poor post-thaw semen quality during the cryopreservation process. Therefore, the present study was designed to investigate the effects of dissolved oxygen (DO) levels (4, 6 and 8 ppm) in semen extender on freezability of crossbred bull spermatozoa. As the gap between 4 and 8 ppm existed in earlier studies, DO of 6 ppm in semen extender was further standardized and the effects were compared with other respective groups to contemplate any improvement in post-thaw semen quality.Methods: For the experiment, Tris-egg Yolk-Glycerol (TYG) extender was partially deoxygenated by nitrogen gassing @ 2-3 bubbles/sec at 34°C for 0, 16, 12 and 9 minutes to obtain DO levels of 11.7 ppm (Group-I/control), 4 ppm (Group-II), 6 ppm (Group-III) and 8 ppm (Group IV), respectively and collected semen samples were diluted with these extender groups to have 80×106 spermatozoa/ ml of the extender. Semen samples were evaluated for individual progressive motility (IPM), lipid peroxidation (LPO), total antioxidant capacity (TAC), plasma membrane integrity, acrosomal integrity and apoptotic changes at different stages of cryopreservation.Result: At the post-thaw stage, progressive motility was greater (p less than 0.05) in Group II compared to Group I and the least reduction from the post-dilution to the post-thaw stage was observed in Group II. In comparison to Group I, Groups II, III and IV showed lesser (p less than 0.05) MDA production with Group II having greater (p less than 0.05) TAC concentration than other groups at the post-thaw stage. A declining trend was observed in membrane integrity as DO levels increased from 4 ppm to 11.7 ppm. Acrosomal integrity did not differ among treatment groups, but, found to be higher (p less than 0.05) than the control group. Per cent viable spermatozoa was greater (p less than 0.05) in Group II than Group I and vice versa for necrotic spermatozoa as assessed by Annexin VFITC/PI staining. In conclusion, reducing the DO level to 4 ppm before cryopreservation improved the freezability by reducing oxidative stress and apoptotic changes while, above 4 ppm tended to lower it. An appreciable improvement in freezability can be seen at 6 ppm of DO, but, not up to that extent as observed at 4 ppm.

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“…Excessive supercooling during the process of freezing results in a rapid ice formation, with the possibility of physical damage resulting in significant deteriorations in seminal parameters, exaggerated by the supra-physiological level of free radicals. Various studies have indicated that male germ cells at various stages of its differentiation (Agarwal and Said, 2003) and through interaction between spermatozoa and dissolved oxygen in extender (Amin et al 2018;Lone, 2018) have the potential to generate reactive oxygen species (ROS) and reactive nitrogen species (RNS). The ROS include singlet oxygen ( 1 O 2 ), hydrogen peroxide (H 2 O 2 ), superoxide anion (O 2 -) and hydroxyl radicals (OH -) (Lone et al 2018), likewise, RNS include nitrogen dioxide (NO 2 ), peroxynitrite (ONOO -) and nitric oxide (NO) (Nash et al 2012), which have been recently implicated in inducing poor sperm function and sperm fertilizing ability in bovines.…”

Section: Introductionmentioning

confidence: 99%

Time Dependent Impact of Reactive Oxidants on Seminal Attributes of Murrah Bull during Cryopreservation and Storage

Upadhyay¹,

Roy²,

Pandita³

et al. 2021

IJAR

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Background: Cryopreservation is an invaluable technique yet it is also known to be detrimental to sperm function and fertility due to cryo-injury and concomitant generation of reactive oxidants. During laboratory manipulation for the cryopreservation and freeze-thaw process, spermatozoa undergo osmotic stress, ionic imbalance, metabolic decoupling, membrane phase transition, destabilization of the cytoskeleton and antioxidant depletion which communally hampers the semen quality.Methods: With the aim of determining implications of cryopreservation and storage, semen samples were collected by artificial vagina technique from 12 Murrah bulls and subsequently examined at 0 hour (before cryopreservation) and at 24 hour, 1 month and 2 month of storage for various seminal attributes. Simultaneously seminal plasma was separated and preserved at -20oC till the analysis of biochemical indicators of semen quality viz., nitric oxide (NO), total antioxidant quantity (TAC) and lipid peroxidation status (TBARS). Result: A sharp reduction (p less than 0.01) in the semen quality was observed only at 24 h after cryopreservation except for viability. Significant reduction (p less than 0.05) in viable counts was observed up to 1 month interval. The capacitated sperm percentage was greater (p less than 0.01) in the cryopreserved semen as compared to fresh ejaculate. The mean ± SE levels of NO (μmol/L), TAC and TBARS (Units/ml) was 2.31±0.27, 0.73±0.04 and 1.11±0.16 respectively in seminal plasma of neat semen stored at -20oC, while the values in the extended seminal plasma after cryopreservation was 2.37±0.31, 0.44±0.03 and 0.65±0.03 respectively. So it can be inferred that most of the damage encountered by spermatozoa is during the initial period of freezing, but the damage associated by various stressors cannot be ignored.

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Effect of various levels of dissolved oxygen on reactive oxygen species and cryocapacitation‐like changes in bull sperm

Cited by 23 publications

References 39 publications

Semen extenders: An evaluative overview of preservative mechanisms of semen and semen extenders

Semen extenders: An evaluative overview of preservative mechanisms of semen and semen extenders

Partial Deoxygenation of Semen Extender Minimizes Post-thaw Damages and Improves Freezability of Crossbred Bull Spermatozoa

Time Dependent Impact of Reactive Oxidants on Seminal Attributes of Murrah Bull during Cryopreservation and Storage

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