Effect of Various Media on Growth and Protease Production in Carica papaya L. Callus Cultures

Bacitracin significantly reduces degradation of peptides in plant cell cultures

“…For example, Ficus carica calli varied in ficin content depending on the hormonal composition of the medium (Nassar and Newbury, 1987), Carica papaya calli varied in protease production depending on which of 20 types of media they were grown (Medora et al, 1984), and suspension cell cultures of Catharanthus roseus had lower protease activity when the cells were grown in nutrient medium complete in phosphate (Ukaji and Ashihara, 1987).…”

Shear rate as scale-up criterion of the protein production with enhanced proteolytic activity by phosphate addition in the Jacaratia mexicana cell culture

“…Under this hydrodynamic condition, the cells were smaller and thinner compared to cell culture in bubble column and airlift bioreactors (40 s −1 ). The phosphate was 1.25 mmol L −1 (the basal phosphate concentration reported in Murashige & Skoog (MS) [15]) in the experiments mentioned above; however, few reports have mentioned that increasing phosphate concentration could improve the protein concentration [16][17][18].…”

Changes in protein and protease activity during the growth and senescence of Paul's Scarlet rose

“…Although numerous studies have dealt with the role of proteases in plants, only a few investigations have reported the protease levels in cultured cells [1,2,3,4], and none of these studies dealt with aging cultures. The purpose of this study was to determine the relationship between the protein content and protease activity present in cultured cells of increasing age.…”