Effect of various parameters on the distribution and extraction of platelets in a microfluidic system

Laxmi, Vijai; Joshi, Suhas S.; Agrawal, Amit

doi:10.1007/s10404-021-02464-5

Cited by 5 publications

(7 citation statements)

References 37 publications

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“…At the start of expansion zone 60 μm wide side channels are provided to collect platelet-rich plasma sample [22]. The systematic development of the devices in our lab has been reported elsewhere [18,[22][23][24].…”

Section: The Microfluidics Devicementioning

confidence: 99%

“…The development and extensive characterization of the microdevices has been reported in various publications [18,[22][23][24]. The microdevice can separate platelet-rich plasma with about 15-fold enrichment of platelets above the normal physiological level.…”

Section: The Microfluidics Devicementioning

confidence: 99%

“…Manipulation of platelets in the plasma Platelets counts can be manipulated based on the application by altering the inlet samples. Reproduced from [24], with permission from Springer Nature. Morphology of platelets post separation ∼95% platelets maintained their disc or spherical shape with a smooth contour and normal size (no adverse effect on the quality of platelets post separation from the microdevice).…”

Section: Platelet-rich Plasma Separation To Suppress the Severitymentioning

confidence: 99%

“…Copyright (2020) American Chemical Society. Note: Images adapted from [22][23][24] by permission from American Chemical Society and Springer Nature.…”

Section: Sn Performance Parameter Performancesmentioning

confidence: 99%

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A lab-on-chip solution for the detection and suppression of COVID-19 severity

Laxmi

Joshi

Agrawal³

2023

Biomed. Phys. Eng. Express

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At present, the conventional method for detecting COVID-19 infection is reverse transcription-polymerase chain reaction (RT-PCR) performed on nasopharyngeal and pharyngeal swabs. In addition, other detection techniques such as isothermal nucleic acid amplification, enzyme-linked immunosorbent assay (ELISA) have also been proposed and are being used. However, these techniques are limited to central hospitals and pathologies. Point-of-care solutions may play an important role in rapid and timely detection by an individual at their doorstep. In addition, the disease adversely affects the functioning of various constituents of human blood. For example, it reduces the platelets count, increases production of proinflammatory cytokine which results in cytokine storm, and increases level of various plasma proteins with the severity of the disease. Therefore, monitoring and transfusion of blood plasma and platelets may play an essential role in detection and suppression of severity of COVID-19 infection. In this regard, we propose evolution of our existing microdevice for the detection and suppression of severity of COVID-19 infection. We propose modification of our microdevice in the following ways (i) as a platelet-rich plasma separation unit, (ii) as an on-chip device to study inter-cellular properties of platelets, and (iii) for on-chip detection of infection by separating plasma. The integration of these microdevices with lateral flow assays, flow-virometry reader (FVR), direct serological biosensor assay along with proprietary technology based on plasmonic, place these microdevices in an advantageous position. Moreover, coupling of the microdevices with machine leaning application for rapid detection of the severity of COVID-19 and platelets related disorders makes these microdevices as a complete unit for point-of-care application.

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Section: The Microfluidics Devicementioning

confidence: 99%

Section: The Microfluidics Devicementioning

confidence: 99%

Section: Platelet-rich Plasma Separation To Suppress the Severitymentioning

confidence: 99%

“…Copyright (2020) American Chemical Society. Note: Images adapted from [22][23][24] by permission from American Chemical Society and Springer Nature.…”

Section: Sn Performance Parameter Performancesmentioning

confidence: 99%

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A lab-on-chip solution for the detection and suppression of COVID-19 severity

Laxmi

Joshi

Agrawal³

2023

Biomed. Phys. Eng. Express

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“…Microfluidicsbased methods have been introduced to overcome the limitations of conventional methods. Microfluidics-based methods report potential in effectively isolating, manipulating blood cells, involving compact size, small sample volume, low cost, and portable microdevice [14,15]. Moreover, they showed no adverse effect on the quality of blood cells post isolation [15].…”

Section: Introductionmentioning

confidence: 99%

Extracting white blood cells from blood on microfluidics platform: a review of isolation techniques and working mechanisms

Laxmi¹,

Joshi²,

Agrawal³

2022

J. Micromech. Microeng.

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Selective isolation of human blood cells has numerous applications in the field of disease diagnostic, prognostics, drug discovery, and drug delivery. In particular, isolation of white blood cells (WBCs) is required for the detection of various diseases such as leukemia, Human immunodeficiency virus (HIV) infection, Epstein–Barr virus (EBV), cancers etc. Although, the conventional methods of centrifugation and flow cytometry are broadly employed for the isolation of WBCs in clinical practice, they experience several limitations such as the requirement of large volume of samples and reagents, trained personnel, large setup, and have adverse effect on the quality of cells. In contrast, microfluidics based methods have appeared as a superior approach of cells isolation with advantages such as low cost, easy to operate, compact in size, and requiring a lower sample volume. In this review, we focus on the various microfluidics techniques for the isolation of WBCs from blood. Here, we have discussed the working mechanism of various microfluidics techniques, microdevice designs, and their performance parameters to isolate WBCs. A detailed discussion on the various forces acting on cells while flowing through microchannels is also provided. In addition, a brief description of the numerous advantages and limitations of the existing microdevices, and their future prospects aiming to develop an affordable, user friendly point-of-care solution is provided.

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Physics of fluid flow in an hourglass (converging–diverging) microchannel

2022

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This work presents the numerical and experimental study of flow physics and characterization in hourglass microchannels at different geometric and flow parameters such as convergence-divergence angle, width ratio, length, aspect ratio, and Reynolds number. The first part of the study discusses the importance of finding a unique length scale to represent an hourglass microchannel. This representative dimension is proposed at L/2.9thfrom the inlet of the microchannel by using a frictional equivalence concept between uniform and hourglass microchannels. This length scale is unique as it remains independent of geometric and flow variables. The study of local flow physics shows that this length scale identifies the region that governs the overall flow behavior of the microchannel. The results also show that the pressure drop is an inverse function of convergence-divergence angle and aspect ratio, whereas width ratio and length are direct functions. In addition, the pressure drop shows linear behavior with volume flow rate (Reynolds number), similar to that of uniform microchannel except at higher volume flow rate for convergence-divergence angle or higher width ratio. This non-linear behavior is explained with the help of hydrodynamic resistance and velocity streamlines in the last part of this study. Furthermore, the convergence-divergence angle and the width ratio are identified as critical parameters to characterize the flow. Overall, the present study gives insights into the influence of the convergence-divergence effect due to critical parameters on the flow characteristics, which could help design hourglass microchannels for many engineering applications.

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Effect of various parameters on the distribution and extraction of platelets in a microfluidic system

Cited by 5 publications

References 37 publications

A lab-on-chip solution for the detection and suppression of COVID-19 severity

A lab-on-chip solution for the detection and suppression of COVID-19 severity

Extracting white blood cells from blood on microfluidics platform: a review of isolation techniques and working mechanisms

Physics of fluid flow in an hourglass (converging–diverging) microchannel

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