Effect of Varying Physicochemical Parameters on the Productivity and Phycobiliprotein 
 Content of Indigenous Isolate Geitlerinema sulphureum

Kenekar, Anuja A.; Deodhar, Manjushri A.

doi:10.3923/biotech.2013.146.154

Cited by 22 publications

(7 citation statements)

References 8 publications

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“…Furthermore, we recorded an increase in the amount of biliproteins as the culture growth period lengthened. Due to their high nitrogen amount, biliprotein synthesis is directly linked and depends on the cell growth status: the more advanced the culture, the more the biliprotein content increases [ 51 , 65 , 66 ]. There are, however, some studies that report increased phycocyanin production induced by high illumination [ 35 , 67 ], but we feel they need further verification.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Colored and White Light on Growth and Phycobiliproteins, Chlorophyll and Carotenoids Content of the Marine Cyanobacteria Phormidium sp. and Cyanothece sp. in Batch Cultures

Hotos

Antoniadis

2022

Life

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Two local marine cyanobacteria, Phormidium sp. and Cyanothece sp., were batch-cultured under 18–19.5 °C, at 40 ppt salinity, using white LED light of low (40 μmol photons/m2/s) and high (160 μmol/m2/s) intensity and, additionally, blue, green and red LED light. Yield was highest in high white light in both species (2.15 g dw/L in Phormidium, 1.47 g/L in Cyanothece), followed by green light (1.25 g/L) in Cyanothece and low white and green (1.26–1.33 g/L) in Phormidium. Green light maximized phycocyanin in Phormidium (0.45 mg/mL), while phycoerythrin was enhanced (0.17 mg/mL) by blue light and allophycocyanin by all colors (~0.80 mg/mL). All colors maximized phycocyanin in Cyanothece (~0.32 mg/mL), while phycoerythrin and allophycocyanin peaked under green light (~0.138 and 0.38 mg/mL, respectively). In Phormidium, maximization of chlorophyll-a (9.3 μg/mL) was induced by green light, while total carotenoids and b-carotene (3.05 and 0.89 μg/mL, respectively) by high white light. In Cyanothece, both white light intensities along with green maximized chlorophyll-a (~9 μg/mL) while high white light and green maximized total carotenoids (2.6–3.0 μg/mL). This study strongly indicates that these cyanobacteria can be cultured at the first stage under white light to accumulate sufficient biomass and, subsequently, under colored light for enhancing phycobiliproteins.

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Section: Discussionmentioning

confidence: 99%

The Effect of Colored and White Light on Growth and Phycobiliproteins, Chlorophyll and Carotenoids Content of the Marine Cyanobacteria Phormidium sp. and Cyanothece sp. in Batch Cultures

Hotos

Antoniadis

2022

Life

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“…Thus, the data showed that sufficient amount of nitrogen is required to maintain phycocyanin levels. Similarly, Kenekar and Deodhar ( Kenekar and Deodhar, 2013 ) reported that increasing nitrate concentration increased biomass and C-PC production of cyanobacteria Geitlerinema sulphureum and achieved maximum biomass production at the concentration of 3.5 g L −1 , while the PC content drastically increased at the concentrations of 3.5 g L −1 (8.8%) and 4.5 g L −1 (9.96%).…”

Section: Discussionmentioning

confidence: 79%

Acclimation and Characterization of Marine Cyanobacterial Strains Euryhalinema and Desertifilum for C-Phycocyanin Production

Khazi

Liaqat

et al. 2021

Front. Bioeng. Biotechnol.

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This study involves evaluation of two native cyanobacterial strains Euryhalinema and Desertifilum isolated from a mangrove pond in Haikou (China) for their possible phycocyanin (C-PC) production. Maximal growth rate with highest chlorophyll and C-PC accumulation were observed at 28°C and 60 μmol photons m−2 s−1 photon flux density for Euryhalinema sp., while for Desertifilum sp. at 32°C and 80 μmol photons m−2 s−1. Nitrogen and iron concentration trails revealed that double strength concentration of sodium nitrate and ferric ammonium citrate in original BG11 media increased growth rate and accumulation of C-PC for both strains. Three different C-PC extraction methods were tested. The combined extraction protocol of freeze–thaw and ultrasonication markedly increased the C-PC extraction efficiency and attained the food grade purity (A620/A280 ratio >0.7), whereas a higher C-PC yield was found with Na-phosphate buffer. Furthermore, the clarified crude extract was used to purify C-PC by fractional ammonium sulfate [(NH₄)₂SO₄] precipitation, Sephadex G-25 gel filtration chromatography, and DEAE-sephadex ion exchange chromatography and attained analytical grade purity (A620/A280 ratio >3.9). Taken together, both strains showed their potential to be domesticated for valuable phycocyanin production.

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“…But the optimum and exponential growth of the culture was seen with a carbonate concentration of 0.2 g/L which was most conducive for the good biomass production and hence it was shortlisted for the optimum growth of the cyanobacterial culture. The effect of various physico-chemical parameters on the biomass productivity and phycocyanin pigment production of the Geitlerinema sulphureum cya- nobacterial strain was also carried out through similar experiments (15). Effect of various carbonate concentrations was checked on the same strain.…”

Section: Effect Of Various Carbonate Concentrations On the Biomass Pr...mentioning

confidence: 99%

Optimization of physico-chemical parameters for the production of phycobilin protein blue pigment, phycocyanin from the cyanobacterial strain Pseudanabaena limnetica (Lemmermann) Komarek

Tribhuvan¹,

Deodhar²,

Kengar³

2023

Plant Sci. Today

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Pseudanabaena limnetica, the cyanophycean microalga, like other members of Cyanophycea, is an excellent source of pigments such as phycocyanin, proteins, carotenoids and polysaccharides. These strains also form a large proportion of algal biomass. The P.limnetica strain can grow in the extreme environmental conditions and it grows well in SW-BG 11 medium under laboratory conditions. In the present investigation, this cyanobacteria strain was isolated from the salt pans of Mulund Mumbai areas and it was cultivated in the lab under controlled conditions of light and temperature with optimum parameters of nitrate and carbonate concentrations. The culture was cultivated in the 60L photo bioreactor systems with the (65,000-85,000 lux) at 45?C in the SW-BG11 medium. The optimization experiments were carried out at the indoor and outdoor conditions. The nitrate and carbonate concentrations were optimized for obtaining maximum amount of algal biomass along with the blue-green phycocyanin pigment. The phycocyanin pigment was lyophilized for its further incorporation into the food and cosmetics products. The spectroscopic calculations of phycocyanin, allophycocyanin and phycoerythrin was done at 620, 650 and 562 nm using the Bennett and Bogorad equation. From the results obtained, it was concluded that 0.1gms/L and 1.5gms/L of the carbonate and nitrate concentrations, respectively, were the ideal concentrations for the further experiments for the cost effective production of P. limnetica in the SW-BG 11 medium. The outdoor conditions were found to be favorable for obtaining the maximum biomass and phycocyanin pigment production which would - make it more cost effective, commercially.

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Effect of Varying Physicochemical Parameters on the Productivity and Phycobiliprotein Content of Indigenous Isolate Geitlerinema sulphureum

Cited by 22 publications

References 8 publications

The Effect of Colored and White Light on Growth and Phycobiliproteins, Chlorophyll and Carotenoids Content of the Marine Cyanobacteria Phormidium sp. and Cyanothece sp. in Batch Cultures

The Effect of Colored and White Light on Growth and Phycobiliproteins, Chlorophyll and Carotenoids Content of the Marine Cyanobacteria Phormidium sp. and Cyanothece sp. in Batch Cultures

Acclimation and Characterization of Marine Cyanobacterial Strains Euryhalinema and Desertifilum for C-Phycocyanin Production

Optimization of physico-chemical parameters for the production of phycobilin protein blue pigment, phycocyanin from the cyanobacterial strain Pseudanabaena limnetica (Lemmermann) Komarek

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