Effect of vat pasteurization, ultra-high temperature sterilization, retort sterilization and homogenization on soluble proteins in donor human milk detected via proteomics

Kim, Bum Jin; Koh, Jeewon; Liang, Ningjian; Yang, Jessica; Ozturk, Gulustan; Dallas, David C.

doi:10.1016/j.lwt.2023.114842

Cited by 7 publications

(2 citation statements)

References 40 publications

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“…These compositional changes may lead to different non-specific complexations and facilitate self-aggregations [44], as visualized in the bright-field images of RTR samples (Figure 5). In our recent collaborative study, which assessed the thermal stability of human milk proteins subjected to the same processing treatments, we observed the disappearance of bands in SDS-PAGE gels with these treatments, and aggregation was confirmed for select proteins through proteomic analysis [45,46]. In addition, the zeta potential of MFG in milk samples was measured to investigate the stability of MFG after various processes.…”

Section: Discussionmentioning

confidence: 93%

Investigating Milk Fat Globule Structure, Size, and Functionality after Thermal Processing and Homogenization of Human Milk

Ozturk,

Paviani,

Rai

et al. 2024

Foods

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Human milk provides bioactive compounds such as milk fat globules (MFGs), which promote brain development, modulate the immune system, and hold antimicrobial properties. To ensure microbiological safety, donor milk banks apply heat treatments. This study compares the effects of heat treatments and homogenization on MFG’s physicochemical properties, bioactivity, and bioavailability. Vat pasteurization (Vat-PT), retort (RTR), and ultra-high temperature (UHT) were performed with or without homogenization. UHT, RTR, and homogenization increased the colloidal dispersion of globules, as indicated by increased zeta potential. The RTR treatment completely inactivated xanthine oxidase activity (a marker of MFG bioactivity), whereas UHT reduced its activity by 93%. Interestingly, Vat-PT resulted in less damage, with 28% activity retention. Sialic acid, an important compound for brain health, was unaffected by processing. Importantly, homogenization increased the in vitro lipolysis of MFG, suggesting that this treatment could increase the digestibility of MFG. In terms of color, homogenization led to higher L* values, indicating increased whiteness due to finer dispersion of the fat and casein micelles (and thus greater light scattering), whereas UHT and RTR increased b* values associated with Maillard reactions. This study highlights the nuanced effects of processing conditions on MFG properties, emphasizing the retention of native characteristics in Vat-PT-treated human milk.

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Section: Discussionmentioning

confidence: 93%

Investigating Milk Fat Globule Structure, Size, and Functionality after Thermal Processing and Homogenization of Human Milk

Ozturk,

Paviani,

Rai

et al. 2024

Foods

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“…Collection of DHM occurs most commonly through milk banks like the Human Milk Banking Association of North America (HMBANA), and donors are given detailed instructions regarding storage and shipment of milk (Abrams et al, 2017 ). Processing of DHM is completed via holder pasteurisation, high‐temperature‐short‐time pasteurisation, high‐pressure processing, ultraviolet‐C irradiation or vat pasteurisation (Kim et al, 2023 ; Moro et al, 2019 ). Distribution occurs from milk banks to NICUs within hospitals, which also receive storage guidelines (Abrams et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

A systematic review of contaminants in donor human milk

Thayagabalu,

Cacho,

Sullivan

et al. 2024

Maternal & Child Nutrition

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Donor human milk (DHM) from a milk bank is the recommended feeding method for preterm infants when the mother's own milk (MOM) is not available. Despite this recommendation, information on the possible contamination of donor human milk and its impact on infant health outcomes is poorly characterised. The aim of this systematic review is to assess contaminants present in DHM samples that preterm and critically ill infants consume. The data sources used include PubMed, EMBASE, CINAHL and Web of Science. A search of the data sources targeting DHM and its potential contaminants yielded 426 publications. Two reviewers (S. T. and D. L.) conducted title/abstract screening through Covidence software, and predetermined inclusion/exclusion criteria yielded 26 manuscripts. Contaminant types (bacterial, chemical, fungal, viral) and study details (e.g., type of bacteria identified, study setting) were extracted from each included study during full‐text review. Primary contaminants in donor human milk included bacterial species and environmental pollutants. We found that bacterial contaminants were identified in 100% of the papers in which bacterial contamination was sought (16 papers) and 61.5% of the full data set (26 papers), with the most frequently identified genera being Staphylococcus (e.g., Staphylococcus aureus and coagulase‐negative Staphylococcus) and Bacillus (e.g., Bacillus cereus). Chemical pollutants were discovered in 100% of the papers in which chemical contamination was sought (eight papers) and 30.8% of the full data set (26 papers). The most frequently identified chemical pollutants included perfluoroalkyl substances (six papers), toxic metal (one paper) and caffeine (one paper). Viral and fungal contamination were identified in one paper each. Our results highlight the importance of establishing standardisation in assessing DHM contamination and future studies are needed to clarify the impact of DHM contaminants on health outcomes.

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Comparative proteomic analysis of donor human milk treated by high-pressure processing or Holder pasteurization on undigested proteins across dynamic simulated preterm infant digestion

Pitino,

O'Connor,

Unger

et al. 2025

Food Chemistry

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Effect of vat pasteurization, ultra-high temperature sterilization, retort sterilization and homogenization on soluble proteins in donor human milk detected via proteomics

Cited by 7 publications

References 40 publications

Investigating Milk Fat Globule Structure, Size, and Functionality after Thermal Processing and Homogenization of Human Milk

Investigating Milk Fat Globule Structure, Size, and Functionality after Thermal Processing and Homogenization of Human Milk

A systematic review of contaminants in donor human milk

Comparative proteomic analysis of donor human milk treated by high-pressure processing or Holder pasteurization on undigested proteins across dynamic simulated preterm infant digestion

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