Effect of vegetable oils on the methane concentration and population density of the rumen ciliate, <i>Eremoplastron dilobum</i>, grown <i>in vitro</i>

Abstract

The effect of rapeseed (RSO) and linseed (LSO) oils on the methane concentration and number of rumen ciliates (Eremoplastron dilobum) incubated in vitro was investigated. A 5% dose of rapeseed oil reduced the methane concentration by about 14% (P<0.05) but had no effect on the ciliate number. Conversely, the same dose of LSO significantly increased the protozoa count, while methane production remained unchanged. No positive correlation was found between the methane concentration and ciliate number or with the inhibitory effect of oils rich in C18:1, C18:3 fatty acids on the population density of Eremoplastron dilobum.

The potential of the wild dog rose (<i>Rosa canina</i>) to mitigate <i>in vitro</i> rumen methane production

“…In carried study, dietary supplementation with this oil did not negatively influence the analysed populations of Entodiniomorpha and Holotricha. With these results we confirmed observations of earlier studies demonstrating none or partial effect of dietary supplementation with C18 rich oils on protozoa population in the rumen ecosystem (Cieślak et al, 2006a;Szumacher-Strabel et al, 2009). Kišidayová et al (2006a) pointed that the rumen protozoa may respond in different ways to oil supplementation in in vitro conditions.…”

Effect of oils rich in linoleic acid on <i>in vitro</i> rumen fermentation parameters of sheep, goats and dairy cows

“…The authors pointed out that rumen ciliates had no uniform response to oil supplements in in vitro studies, which also might explain the differences in our results (sheep and goat). Furthermore, Cieślak et al (2006) stressed that vegetable oils differently affected the rumen ciliate Eremoplastron dilobum, grown in vitro. Additionally, an unpublished study of ours indicates that the effect of linoleic acid depended on the dose and form of the supplement.…”

Dietary Possibilities to Mitigate Rumen Methane and Ammonia Production

“…Entodinium elonginucleatum, Entodinium bursa, Eudiplodinium maggii and Eremoplastron bovis. In other studies it was shown that the symbiosis of protozoans and methanogens may be observed in the ruminal ecosystem in case of Dasytricha ruminantium, Entodinium caudatum, Entodinium furca monolobum, Diplodinium dentatum, Eremoplastron dilobum, Metadinium medium, Epidinium ecaudatum and Ophyroscolex caudatus Kisidayova et al, 2000;Regensbogenova et al, 2004b;Cie lak et al, 2006a). Literature data seem to be ambiguous e.g.…”

“…The EE and EM control monocultures produced on the average higher amounts of methane (0.77 and 0.74 mmol, respectively) when compared to the EC control (0.54 mmol). The above hypotheses may be partly explained by a lack of a negative effect of the addition of oils on the population of the analyzed rumen ciliates in case of some studies (Matsumoto et al, 1991;Cie lak et al, 2006a;Kisidayova et al, 2006;Cie lak et al, 2009a). A 5% addition of evening primrose oil containing considerable amounts of C18:2 and C18:3 caused a reduction of the counts of Dasytricha ruminantium, Isotricha spp.…”