Effect of vibration emissions during shipping of artificial insemination doses on boar semen quality

Schulze, Martin; Bortfeldt, Ralf; Schäfer, Jana; Jung, Markus; Fuchs-Kittowski, Frank

doi:10.1016/j.anireprosci.2018.03.035

Cited by 29 publications

(19 citation statements)

References 25 publications

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“…However, the susceptibility for cold shock is dependent on many different factors, and recently there have been reports of successfully sustained sperm quality at low temperature through optimization of surrounding conditions 15 , 35 , 36 . Amongst others, boar individual eligibility 37 , 38 , the cooling rate 15 , 39 , the used extender 40 , motionless storage 41 and transport 42 play important roles in the maintenance of sperm quality.…”

Section: Discussionmentioning

confidence: 99%

Low temperature preservation of porcine semen: influence of short antimicrobial lipopeptides on sperm quality and bacterial load

Hensel¹,

Jakop²,

Scheinpflug

et al. 2020

Sci Rep

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Antimicrobial resistance is a steadily increasing problem and poses a serious threat to global public health. Therefore, it is highly necessary to advance the development of novel antimicrobial compounds and semen preservation strategies. The aim of this study was to evaluate a low temperature, antibiotic-free preservation procedure using Androstar Premium (ASP) extender (Minitüb) with antimicrobial lipopeptides. Firstly, seven lipopeptides in two concentrations (1 × minimum inhibitory concentration (MIC)/2 × MIC) were tested on their sperm-compatibility at 17 °C. Two lipopeptides, C16-KKK-NH 2 and C16-KKKK-NH 2 , did not negatively affect sperm quality and were further evaluated for their efficiency of bacterial growth inhibition at 5 °C. Besides an overall diminution of colony forming units, both peptides showed a reduction of bacterial subcultures (n = 103) with a decrement in Gram-positive rods from 65 (ASP w/o supplements) to 39/52 (ASP w/ C16-KKK-NH 2 /C16-KKKK-NH 2), in Gram-positive cocci from 21 to 9/10 and in Gram-negative species from 17 to 8/5 total subcultures. Furthermore, lipopeptides revealed activity towards selected bacteria of potential concern in artificial insemination like Trueperella pyogenes, Alcaligenes faecalis, Pseudomonas aeruginosa (not C16-KKK-NH 2), Pasteurella sp., Providencia stuartii, Escherichia coli (not C16-KKKK-NH 2) and Streptococcus porcinus (not C16-KKKK-NH 2). Consequently, both tested lipopeptides are promising candidates for alternative antibiotic-free preservation techniques of boar semen. Spreading antimicrobial resistance is a growing problem in human and veterinary medicine, poses a serious threat to global public health 1 and should therefore be addressed in a multifaceted approach from all areas of concern, including the industry branch of artificial insemination (AI) in pigs. It has been proven, that unhindered bacterial growth has detrimental effects on several sperm quality characteristics 2 and a transmission of potential pathogens to the inseminated sow has to be prevented in order to minimize negative effects on fertility and litter size 3. Semen collection is a non-sterile process and AI doses are conventionally stored at temperatures between 16 and 18 °C to prevent cold shock injuries to the highly sensitive boar spermatozoa 4. The combination of moderate temperatures and the use of nutrient-rich semen extenders promotes bacterial growth of psychrophilic and mesophilic species 5. As a countermeasure, antibiotics like gentamicin sulphate are routinely added to boar semen preserved for AI (Council Directive, European Union, 90/429/EEC). A steadily growing portion of contaminant bacteria isolated from AI doses are resistant to antibiotics commonly used as additives in semen extenders 6 , threatening animal welfare. This has far-reaching consequences for public health because of the natural backflow of parts of the rather large-volumed AI doses after insemination 7 ,

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Section: Discussionmentioning

confidence: 99%

Low temperature preservation of porcine semen: influence of short antimicrobial lipopeptides on sperm quality and bacterial load

Hensel¹,

Jakop²,

Scheinpflug

et al. 2020

Sci Rep

Self Cite

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“…In pig production, the usage of frozen semen for AI reduced litter size of sows [5,6]. However, it still has many advantages in pig production and breeding processes such as longterm preservation of germplasm resources, the exchange of germplasm resources between home and abroad, no limitation by time and place in insemination of oestrous sows, and so on [7,8]. Moreover, frozen semen can replace fresh semen in in vitro fertilization (IVF) tests [9].…”

Section: Introductionmentioning

confidence: 99%

DMEM and FBS as thawing solutions for frozen semen of pigs can improve sperm motility and sow reproductive performance

Zheng¹,

Li²,

Huang³

et al. 2020

Turk J Vet Anim Sci

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The purpose of this experiment was to investigate the effects of Beltsville thawing solution (BTS), Androhep, and Dulbecco's modified Eagle medium + Fatal bovine serum (D-F) as diluents of frozen semen on sperm motility and sow reproductive performance. In experiment 1, boar semen was collected and diluted to 2.0 × 10 9 /60 mL, 1.5 × 10 9 /60 mL, and 1.5 × 10 9 /40 mL with Androhep. The appropriate semen dose was determined by postcervical insemination. In experiment 2, boar semen was fumigated with liquid nitrogen and thawed semen was diluted to 1.5 × 10 9 /40 mL with D-F, Androhep, and BTS, respectively. The effects of above diluents on sperm motility and sow reproductive performance were studied by postcervical insemination. The appropriate semen dose for postcervical insemination was 1.5 × 10 9 /40 mL. After thawing of frozen semen, sperm motility of semen diluted with D-F was significantly higher than that of semen diluted with Androhep and BTS. There was no significant difference in pregnancy rates among sows via artificial insemination. The litter size of sows using D-F diluted semen was significantly higher than that of sows using Androhep and BTS diluted semen. In conclusion, D-F freezing diluent can improve sperm motility of frozen boar semen after thawing, prolong sperm survival time, and increase sow reproductive performance.

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“…Artificial insemination (AI) has been widely used in the large‐scale modern boar breeding industry (Yeste, Rodriguez‐Gil, & Bonet, ). Sperm quality is an important factor that affects the success rate of AI (Schulze, Bortfeldt, Schafer, Jung, & Fuchs‐Kittowski, ). The recent development in semen preservation technology has solved the problem of long‐term sperm storage (Li et al, ).…”

Section: Introductionmentioning

confidence: 99%

Effects of sulfanilamide on boar sperm quality, bacterial composition, and fertility during liquid storage at 17°C

Feng

Ren

Qian

et al. 2019

Animal Science Journal

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Sulfanilamide (SA) is an effective broad‐spectrum antibacterial agent in human and veterinary medicine. The purpose of this study was to evaluate the effects of SA on boar sperm quality during liquid storage at 17°C and determine the optimal concentration of SA and its effects on bacterial growth, microbial composition, and maternal fertility. Boar ejaculates were diluted with a basic extender, containing different concentrations of SA, and stored in a 17°C incubator for 6 days. The sperm motility, plasma membrane integrity, and acrosome integrity were measured daily. The results showed that when the concentration of SA was 0.02 g/L, the sperm quality parameters were significantly higher than those of all other treatment groups (p < .05). We also monitored the bacterial growth and compared the differences in the microbial species between the 0.02 g/L SA group and the control by 16S rDNA sequencing. The results revealed that some bacteria, such as Staphylococcus and Pseudomonas, were considerably lower in the 0.02 g/L SA group than in the control group (p < .05). In addition, preserved semen was used for artificial insemination, and results showed that 0.02 g/L SA group had a higher litter size, and its pregnancy rate was 92.5%.

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Effect of vibration emissions during shipping of artificial insemination doses on boar semen quality

Cited by 29 publications

References 25 publications

Low temperature preservation of porcine semen: influence of short antimicrobial lipopeptides on sperm quality and bacterial load

Low temperature preservation of porcine semen: influence of short antimicrobial lipopeptides on sperm quality and bacterial load

DMEM and FBS as thawing solutions for frozen semen of pigs can improve sperm motility and sow reproductive performance

Effects of sulfanilamide on boar sperm quality, bacterial composition, and fertility during liquid storage at 17°C

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