Effect of Vibrio alginolyticus on larval survival of the blue mussel Mytilus galloprovincialis

Abstract

The effect of increasing concentrations of Vibrio alginolyticus on survival of Mytilus galloprovincialis larvae was studied in a 48 h static bioassay in 1 l glass bottles. Five bacterial densities were tested ranging from 10(2) to 10(6) bacteria ml(-1). Larval survival and normality (veliger larvae with the typical D-shape) were evaluated after 48 h. An inverse relationship between bacterial concentration and larval survival and normality was observed. In spite of high larval survival (79%) under conditions of high bacterial density (10(5) bacteria ml(-1)), the percent of normal larvae was 11%. Besides an irregular shape, abnormal larvae also presented velum reduction. Results from this study suggest that concentrations of V. alginolyticus lower than 10(3) bacteria ml(-1) should be maintained during M. galloprovincialis larval culture.

Prophylactic effect ofHaslea ostreariaculture supernatant containing the pigment marennine to stabilize bivalve hatchery production

“…The marennine effect was also unequal in reducing V. splendidus pathogenicity toward the species. Larvae of different species may not be as easy to rear (scallops are known to be difficult), and pathogenic effects of the same bacteria will not always be the same for different hosts (Anguiano-Beltrán et al 2004).…”

Two pathogens of Greenshell™ mussel larvae, Perna canaliculus: Vibrio splendidus and a V. coralliilyticus/neptunius‐like isolate

“…To our knowledge this is the first report on bacterial pathogens of marine mussel larvae. Anguiano‐Beltrán, Lizárraga‐Partida and Searcy‐Bernal (2004) reported on a sub‐lethal effect caused by V. alginolyticus on blue mussel larvae, Mytilus galloprovincialis , but did not describe virulent pathogenesis.…”

First Report of Vibrio tubiashii Associated with a Massive Larval Mortality Event in a Commercial Hatchery of Scallop Argopecten purpuratus in Chile

“…Vibrio species have been described worldwide as the main aetiological agents of bacterial pathologies affecting reared larvae of various shellfish species ( Waechter et al, 2002 ; Anguiano-Beltrán et al, 2004 ; Estes et al, 2004 ; Gay et al, 2004 ; Gómez-León et al, 2005 ; Prado et al, 2005 , 2014 , 2015 ; Labreuche et al, 2006 ; Garnier et al, 2007 ; Rojas et al, 2015a ; Dubert et al, 2016b ). Clinical symptoms commonly exhibited by reared shellfish larvae affected by vibriosis include the reduction of larval motility, erratic swimming, closing of valves, velum detachment, and bacterial swarming inside and around the larvae ( Prado et al, 2005 ; Beaz-Hidalgo et al, 2010 ; Rojas et al, 2015a ).…”