Effect of vitamin A deficiency on the levels of glutathione and glutathione-S-transferase activity in rat lung and liver

Dogra, Sunil; Khanduja, Krishan Lal; Sharma, Rati

doi:10.1007/bf01953643

Cited by 11 publications

(3 citation statements)

References 9 publications

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“…Our earlier studies in this area (Dogra et al, 1982;Dogra et al, 1983) (Dogra et al, 1984). These studies give support to the argument that mixed-function oxidase enzymes play a very crucial and important role in determining organ susceptibility to tumourigenesis.…”

Section: Discussionmentioning

confidence: 81%

The effect of vitamin A deficiency on the initiation and postinitiation phases of benzo(a)pyrene-induced lung tumourigenesis in rats

Dogra¹,

Khanduja²,

Gupta³

1985

Br J Cancer

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Summary The present investigation shows the effect of vitamin A deficiency on the initiation and postinitiation phases of benzo(a)pyrene-induced lung carcinogenesis in male Wistar rats. Lung tumours were induced by giving three intratracheal instillations, one week apart, of 10mg benzo(a)pyrene per instillation. Maximum tumour incidence (100%) and tumour burden per rat was found in rats which were kept on vitamin A deficient diet for 4 weeks prior to the first administration and 8 weeks after the last administration of benzo(a)pyrene. Rats in which vitamin A deficiency was terminated after the last administration of the carcinogen had 83% tumour incidence, whereas corresponding control pairfed animals had 39% incidence of tumours. These data represent the values obtained 32 weeks after the last administered dose of the carcinogen and indicate the role of vitamin A, both in the initiation as well as in the postinitiation phases of lung carcinogenesis.

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Section: Discussionmentioning

confidence: 81%

The effect of vitamin A deficiency on the initiation and postinitiation phases of benzo(a)pyrene-induced lung tumourigenesis in rats

Dogra¹,

Khanduja²,

Gupta³

1985

Br J Cancer

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“…The opposite effects of vitamin A deficiency on pulmonary phase I enzymes and UDP-GT activity may be due to different regula tions of the mechanism of biosynthesis be tween UDP-GT on the one hand and cyto chrome P-450 on the other as indicated by Cresteil et al [6]. Earlier, we have observed that pulmonary content of glutathione and activity of glutathione-S-transferase is markedly decreased in vitamin A deficient rats [8], Conjugation of reactive PAH metab olites with glucuronic acid and glutathione, catalyzed by UDP-GT and glutathione-Stransferases are one of the most important phase II reactions [10]. Therefore, it is con ceivable that decrease in activities of UDP-GT and glutathione-S-transferase would in crease the formation of diolepoxide as a re sult of impairment in alternate pathways of conjugation for the primary epoxides and phenols.…”

Section: Body Weight Organ Weight and Hepatic Microsomal And Vitamimentioning

confidence: 88%

Effect of Vitamin A Deficiency on the Pulmonary and Hepatic Drug-Metabolizing Enzymes in Rat

Sc¹,

Khanduja²,

Mp³

et al. 1983

Enzyme

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The effect of vitamin A deficiency on the drug-metabolizing enzyme system of the lung and liver was analyzed in rats fed diets with or without vitamin A for 5-6 weeks. The hepatic level of vitamin A was significantly reduced in vitamin A deficient animals. The hepatic cytochrome P-450 and b(5) contents and activity of benzo(a)pyrene hydroxylase was significantly reduced in deficient animals. Contrary to this, pulmonary cytochrome P-450 and b(5) contents were above the control values. No alteration in pulmonary benzo(a)pyrene hydroxylase was noted. The uridine diphosphate-glucuronosyltransferase activity of digitonin- treated microsomal membranes was below the control values both in lung and liver. However, the native uridine diphosphate-glucuronosyltransferase activity remained unchanged in the liver and was below control values in the lung.

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“…GPX proteins along with SOD and catalase are part of the enzymatic defense system that cells use to reduce free radical-mediated cellular injury. Vitamin A deficiency leads to lower GSH/GSSG ratio in rat liver ( Barber et al, 2000 ), less activity of glutathione-S-transferase in lung ( Dogra et al, 1982 ), and less lung SOD and catalase ( Dogra et al, 1983 ). An antioxidant mechanism of vitamin A may be attributed to transcriptional regulation of antioxidant genes.…”

Section: Discussionmentioning

confidence: 99%

Vitamin A Protects the Preterm Lamb Diaphragm Against Adverse Effects of Mechanical Ventilation

et al. 2018

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Background: Preterm infants are deficient in vitamin A, which is essential for growth and development of the diaphragm. Preterm infants often require mechanical ventilation (MV) for respiratory distress. In adults, MV is associated with the development of ventilationinduced diaphragm dysfunction and difficulty weaning from the ventilator. We assessed the impact of MV on the preterm diaphragm and the protective effect of vitamin A during MV.Methods: Preterm lambs delivered operatively at ∼131 days gestation (full gestation: 150 days) received respiratory support by synchronized intermittent mandatory ventilation for 3 days. Lambs in the treated group received daily (24 h) enteral doses of 2500 IU/kg/day vitamin A combined with 250 IU/kg/day retinoic acid (VARA) during MV, while MV control lambs received saline. Unventilated fetal reference lambs were euthanized at birth, without being allowed to breathe. The fetal diaphragm was collected to quantify mRNA levels of myosin heavy chain (MHC) isoforms, atrophy genes, antioxidant genes, and pro-inflammatory genes; to determine ubiquitin proteasome pathway activity; to measure the abundance of protein carbonyl, and to investigate metabolic signaling.Results: Postnatal MV significantly decreased expression level of the neonatal MHC gene but increased expression level of MHC IIx mRNA level (p < 0.05). Proteasome activity increased after 3 days MV, accompanied by increased MuRF1 mRNA level and accumulated protein carbonyl abundance. VARA supplementation decreased proteasome activity and FOXO1 signaling, down-regulated MuRF1 expression, and reduced reactive oxidant production.Conclusion: These findings suggest that 3 days of MV results in abnormal myofibrillar composition, activation of the proteolytic pathway, and oxidative injury of diaphragms in mechanically ventilated preterm lambs. Daily enteral VARA protects the preterm diaphragm from these adverse effects.

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Effect of vitamin A deficiency on the levels of glutathione and glutathione-S-transferase activity in rat lung and liver

Cited by 11 publications

References 9 publications

The effect of vitamin A deficiency on the initiation and postinitiation phases of benzo(a)pyrene-induced lung tumourigenesis in rats

The effect of vitamin A deficiency on the initiation and postinitiation phases of benzo(a)pyrene-induced lung tumourigenesis in rats

Effect of Vitamin A Deficiency on the Pulmonary and Hepatic Drug-Metabolizing Enzymes in Rat

Vitamin A Protects the Preterm Lamb Diaphragm Against Adverse Effects of Mechanical Ventilation

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