Effect of vitamin-antioxidant micronutrients on the frequency of spontaneous and in vitro γ-ray-induced micronuclei in lymphocytes of donors: the age factor

Abstract

The effect of prolonged consumption of a vitamin-antioxidant mixture (VAM) on the frequency of spontaneous and in vitro gamma-radiation-induced micronuclei (MN) in peripheral blood lymphocytes in donors of various ages was investigated. Three groups of donors were recruited: (i) 56-83 years old (35 subjects), (ii) 23-30 years old (13 subjects), and (iii) 63-82 years old (12 subjects). Blood was sampled every 4 months for one year in all donors of the three groups. After the first sampling of blood, the donors of groups (i) and (ii) took VAM containing the vitamins A, C, E, as well as beta-carotene, folic acid, and rutin daily for 4 months. After the second blood sampling, the intake of VAM was terminated. The third blood sample was taken 4 months after termination of VAM intake. A part of the blood was exposed to gamma-radiation and the frequency of spontaneous and induced MN in lymphocytes was assayed. The analyses showed that the frequency of spontaneous and in vitro gamma-ray-induced MN in aged donors was significantly higher than that in young donors. No seasonal variations in MN frequency were observed in human lymphocytes during one year. Aged donors showed a statistically significant decrease in spontaneous MN in lymphocytes after a 4 month period of consumption of VAM. The intake of VAM by both aged and young donors promoted a decrease in MN induced lymphocytes in vitro by gamma-radiation. The results of our observations enable the suggestion that consumption of VAM favours a decrease in the chromosome damage produced by endogenous and exogenous factors in human lymphocytes.

Antigenotoxic Effect Of Ferulic Acid In 7,12-Dimethyl Benz(A)- Anthracene (Dmba) Induced Genotoxicity

“…Micronucleus assay is a valuable complement to the chromosome aberration measurement and is comparatively easy to perform and inexpensive. Scientific studies have suggested that the presence of one or more micronuclei in peripheral erythrocytes in rodent is an accepted marker of chromosomal breakage or loss that occurred prior to the extrusion of the nucleus during erythrocyte differentiation (Venkatesh et al, 2007;Gaizev et al, 1996). , 1983).…”

“…A hallmark of neoplastic transformation is the accumulation of genetic lesions and is characterized by having aberrant chromosomes. The degree of chromosomal aberrations is correlated with tumor progression (Cheung and Loeb, 1997 (Gaizev et al, 1996). Cancer patients are often exposed to high level of DNA damaging agents.…”

Normal Tissue Protectors Against Radiation Injury

“…Post-irradiation administration of vitamin C protected against wholebody irradiation-induced chromosome damage in mouse 38 . Dietary antioxidant supplementation with vitamins E, C, b-carotene, rutin, zinc and selenium reduced the frequency of radiation-induced micronuclei in human lymphocytes 39 . Combined administration of vitamins E, C and b-carotene before or after irradiation significantly reduced the radiation-induced micronuclei in mouse bone marrow 40 .…”

Genome Destabilization Upon Exposure to Ionizing Radiation and During Acute Blood Loss

“…Тест объектами для изучения цитогенетичес ких повреждений с помощью МЯ теста in vitro яв ляются клетки первичных и перевиваемых клеточ ных культур животных и человека: лимфоциты периферической крови [24], фибробласты кожи человека, фибробласты китайского хомячка, клет ки растений [25] и т. д. Для каждого объекта суще ствует своя специфика приготовления и окраски цитологических препаратов и подсчета МЯ.…”