Effect of vitamin B12 on the oxidative stress of Sitophilus oryzae under different time course

Abstract

The use of vitamins is widely distributed nowadays to overcome the deleterious impacts of oxidative stress especially along ecosystem structure and function. Sitophilus oryzae is considered as a serious pest to all stored products. The deleterious insect infestation may result from the presence of oxidative stress. To decline the impacts of oxidative stress and restore the homeostasis between antioxidants and oxidants, vitamin B12 was used. Here, the effect of vitamin B12 on the insects oxidative stress status was examined in adult insect S. oryzae. Around 250 insects were treated with 2 µg/mL commercial vitamin B-12 under different time course (0, 6, 12, 18, and 24 h). The results showed that the time course has a direct positive elevation effect on the total antioxidant capacity, DPPH, and reducing power of S. oryzae. While, the oxidative stress parameters showed a fluctuation pattern of insect protein carbonyls and lipid peroxides. The highest application time of 2 µg/mL vitamin B12 (24 h) resulted in a drastic depletion in the levels of protein carbonyls amount with the factor of -2.1-x with respect to control value (p < 0.001). These findings suggest the ability of insects to protect against oxidant production.