Effect of Vitamin C Deficiency on Action of Different Types of Barbiturates

Richards, R. K.; Keuter, Kelly A.; Klatt, T. J.

doi:10.1097/00000542-194205000-00039

Cited by 1 publication

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“…It is shown that pentobarbital sleeping time reflects the activity of the MFO system and the AsA deficiency prolongs the sleeping time and plasma half-lives of various drugs [27,28]. In this study, the two AsA-deficient and low AsA diets prolonged the sleeping time, as compared with the control, high and excess levels.…”

Section: Discussionmentioning

confidence: 56%

Influence of Excessive Ascorbic Acid Dose on Liver Microsomal Mixed Function Oxidase System in Guinea Pigs

Saito

Yamaguchi

1988

J. Clin. Biochem. Nutr.

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In the first experiment, guinea pigs were fed diets containing graded levels of ascorbic acid (AsA) from deficient to excess (0, 6, 60, 600, 6,000, and 12,000 mg/kg of diet) for 15 days after pre-feeding with a low AsA diet (60 mg/kg) for one week. Pentobarbital sleeping time measured on day 9 was shortened in the 600-to 12,000-mg AsA groups as compared with the two AsA deficient (0 and 6 mg) and 60-mg AsA groups. Liver microsomal cytochrome P-450 content increased with a dose-response relation to the dietary AsA level except in the excess AsA group (12,000 mg/kg). However, cytochrome b5 content and NADPH-cytochrome c reductase activity showed no significant response. Activities of aminopyrine N-demethylase and aniline hydroxylase showed the same response as seen in cytochrome P-450 content, but to a lesser extent. In the second experiment, guinea pigs were fed the diet containing the normal level of or excess AsA (300 or 24,000 mg/kg of diet, respectively) for 4, 9 or 15 days after a pre-feeding with the normal AsA diet for one week. No significant differences in the time courses of those responses described above were shown between the two AsA groups. In conclusion, the liver microsomal mixed function oxidase activity did not change even transiently during a short period after administration of a large excess of AsA such as 24,000 mg/kg of diet, and 300 mg AsA/ kg of diet is enough to ensure normal levels of the liver microsomal mixed function oxidase system in guinea pigs.

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Section: Discussionmentioning

confidence: 56%