Effect of vitamin D supplementation on cold restraint induced oxidative stress in rats

Abstract

Free radicals are electrically charged molecules that are produced during the course of normal metabolism. They spontaneously attack lipids, proteins, nucleic acids and other biologically important molecules. Polyunsaturated lipids are particularly sensitive to their attack. Free radicals and oxidative stress have been reported to be involved in tissue injury associated with a number of diseases. The present study was done to investigate the effect of vitamin D on cold induced oxidative stress in rats. Rats were divided into control, acute and chronic stress groups with and without vitamin D treatment. Tissue total antioxidant concentration and tissue lipid peroxide were measured in all groups. Statistical analysis was done using the computer software program prism version 3.3. Result showed that the acute cold restraint decreased the total antioxidant levels below the control but there was a significant increase in antioxidant levels of the group in which vitamin D is administered. Chronic cold restraint produces non significant decrease of total antioxidant levels. There was a significant increase of total antioxidant level in chronic cold restraint rats treated vitamin D. Moreover, the chronic cold group with vitamin D produced significant increase of plasma total antioxidant levels above control, acute, chronic groups. The lipid peroxide levels were increased significantly in acute cold restraint group with and without vitamin D as compared to control. Vitamin D produces non significant decrease in lipid peroxide levels in acute and chronic cold restraint group.

Preventive effect of omega‐3 fatty acids in a rat model of stress‐induced liver injury

“…Acute stress hormones, GCs and catecholamines, produce metabolic changes in the enzyme activities of glucose and phosphate pathways. Thus, the decrease in antioxidant levels may be due to the decrease in glucose‐6‐phosphate dehydrogenase activity which causes the diminished production of NADPH and in consequence antioxidant enzymes (Elewa et al, ).…”

“…CRS group: where each rat was administered saline (Marsman et al, ) daily for 4 weeks by oral gavage then subjected to CRS (Dekanski et al, ) for 6 hr for one stress session (Elewa, Rafeeq, & Mohiuddin, ). Rats were killed by decapitation immediately after stress exposure.…”

“…Rats were killed by decapitation. Omega‐3 group: rats were pretreated with menhaden fish oil as a source of omega‐3 fatty acids at a dose of 0.4 g/kg body weight daily (Ashtiyani, Najafi, Kabirinia, Vahedi, & Jamebozorky, ) for 4 weeks by oral gavages (Abdel & Yacoub, ). Rats were killed by decapitation. CRS group: where each rat was administered saline (Marsman et al, ) daily for 4 weeks by oral gavage then subjected to CRS (Dekanski et al, ) for 6 hr for one stress session (Elewa, Rafeeq, & Mohiuddin, ). Rats were killed by decapitation immediately after stress exposure. Omega‐3 + CRS group: rats were pretreated with menhaden fish oil as a source of omega‐3 fatty acids at a dose of 0.4 g/kg body weight daily (Ashtiyani et al, ) for 4 weeks by oral gavages (Abdel & Yacoub, ) before exposure to CRS.…”

Modulatory role of N-acetyl-cysteine on gastric mucosal lesions and some biochemical changes in Wistar rats subjected to cold restraint stress

“…Stress is an adaptive physiological response to disruption of homeostasis (Guo et al, 2012). An imbalance between free radical generation and various antioxidant defense systems leading to the accumulation of free radicals is called oxidative stress (Elewa et al, 2012). The exposure to cold restraint stress (CRS) situations can stimulate numerous pathways, leading to increased production of oxygen free radicals that generate a cascade of reactions producing lipid peroxidation.…”