Effect of vitamin E supplementation on semen and blood profile of vaccinated crossbred bulls

Abstract

Vaccination is one of the major stress factors that affects the semen productivity of the breeding bulls who showed consistent higher body temperature for several days. Therefore, a study was planned to assess the ameliorative strategy of vaccination stress by Vitamin E supplementation on twelve Holstein-Friesian crossbred bulls (432 ejaculates) maintained at Artificial Breeding Research Centre, ICAR-National Dairy Research Institute, Karnal. The bulls were vaccinated for FMD @ 3 ml by SC injections. In treatment group (6 bulls), Vitamin E was supplemented @ 4000 I.U./bull/day, ten weeks before and nine weeks after vaccination and another group without Vitamin E supplementation served as control. Vitamin E supplementation resulted in significantly reduced sperm abnormalities and improved sperm concentration, total motile spermatozoa (106), total live spermatozoa per ml (106), total sperm (106), live sperm/ml and total dose harvested in treated group as compared to control. Individual motility (%), mass motility, NEC (%), HOST (%) and IA (%) were higher in the treatment group, but it was not significant. Better blood biochemical profile of bull was evident in Vitamin E supplemented group though it was not significant statistically. It can be concluded that Vitamin E supplementation in vaccinated bulls was effective in ameliorating vaccination stress to some extent.

Effect of FSH dose on the super ovulatory response in Punganur Cows

“…The overall mean number of ovulations on the right and left ovaries were 1.00 ± 0.38 and 0.86 ± 0.26, respectively, with total ovulations of 1.86 ± 0.40 per animal, In Group II were 1.57 ± 0.37, 1.14 ± 0.34 respectively, with total ovulations of 2.71 ± 0.57 per animal (Table 1). In other indigenous breeds like Ongole (Rao et al 2005) [20] and Kankrej (Sahatpure and Mehta, 2004) [23] the ovulations were more than the present findings which were superovulated with 400 mg FSH. Contrary to the present findings, good response of 18 ovulations out of 21 follicles per animal was recorded by Singhal et al (2017) [25] .…”

“…Similar recovery rate reported in Sahiwal by Mishra et al (2002) [16] with decreasing doses of FSHp and in Iranian Bos Indicus by Barati et al (2006) [4] with constant doses of FSHp. But in Sahiwal (Ullah et al 1998) [30] , Vechur (Venkatachalapathy et al 2000) [32] , Ongole (Kasiraj et al 2000 [11] : Rao et al 2005 [20] ) and Kankrej cows (Sahatpure and Mehta, 2004) [23] higher embryo recovery was reported with 400 mg of FSHp. In the present study, there was no significant difference between the two treatments in the number of embryos recovered.…”