Effect of vitrification on meiotic maturation, mitochondrial distribution and glutathione synthesis in immature silver fox cumulus oocyte complexes

Cao, Xin; Li, Jingchun; Xue, Hailong; Wang, Shiyong; Zhao, Wenjing; Du, Zhanyu; Yang, Yumei; Yue, Zhengkai

doi:10.1016/j.theriogenology.2016.12.037

Cited by 15 publications

(9 citation statements)

References 60 publications

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“…In addition, the present study demonstrated that LBP may assist in recovering mitochondrial function in previously-cryopreserved embryos via increasing expression of SIRT1 and pAMPK, both of which have key roles in mitochondrial biogenesis [3, 20]. Such results are consistent with those of previous animal model studies that demonstrated an LBP-induced increase in SIRT1 [21] and pAMPK expression [16].…”

Section: Discussionsupporting

confidence: 91%

“…Mitochondria are membrane-enclosed organelles that play important roles in a variety of cellular activities, including - among many others - maintenance of cellular homeostasis, adenosine triphosphate (ATP) synthesis, and regulation of apoptosis. Previous studies have shown that vitrification during cryopreservation induces altered mitochondrial distribution and decreased mitochondrial potential (MMP) in oocytes [3] and embryos [4], thereby impairing fertilization and embryo development [3]. Mitochondria are closely associated with the generation and scavenging of ROS [4].…”

mentioning

confidence: 99%

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<i>Lycium barbarum</i> polysaccharide enhances development of previously-cryopreserved murine two-cell embryos via restoration of mitochondrial function and down-regulated generation of reactive oxygen species

Yang

Gao

Lei

et al. 2019

Journal of Reproduction and Development

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Lycium barbarum polysaccharide (LBP) exhibits multiple pharmacological and biological effects, including displaying antioxidant and cytoprotective properties. The current study investigated the effects of LBP-supplemented culture medium on mitochondrial distribution, mitochondrial membrane potential (MMP), adenosine triphosphate (ATP) production, mitochondrial deoxyribonucleic acid (mtDNA) copy number, reactive oxygen species (ROS) accumulation, and development of previously-cryopreserved murine two-cell embryos. Results indicate that LBP enhances development of such embryos, and that potential mechanisms include: (1) mitochondrial function enhancement via altering mitochondrial distribution and increasing MMP, ATP production, mtDNA copy number, and expression of genes involved in mitochondrial biogenesis and energy metabolism (NAD-dependent deacetyltransferase sirtuin-1 (SIRT1) and phosphorylated adenosine monophosphate-activated protein kinase (pAMPK)); (2) down-regulation of ROS generation and enhanced expression of the antioxidant genes glutathione peroxidase 4 (GPX4) and superoxide dismutase 1 (SOD1), thereby increasing embryo oxidative stress tolerance; and (3) increased expression of B-cell lymphoma-2 (BCL2), a critical gene for cell survival and embryo development. These results demonstrate that LBP improves development of previously-cryopreserved murine two-cell embryos via restoration of mitochondrial function and down-regulated generation of ROS.

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Section: Discussionsupporting

confidence: 91%

mentioning

confidence: 99%

<i>Lycium barbarum</i> polysaccharide enhances development of previously-cryopreserved murine two-cell embryos via restoration of mitochondrial function and down-regulated generation of reactive oxygen species

Yang

Gao

Lei

et al. 2019

Journal of Reproduction and Development

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“…However, the survival rate of oocytes and their subsequent developmental competence are decreased significantly after vitrification when compared with these characteristics of fresh oocytes [11,12,13]. The decreased developmental potential due to oocyte cryopreservation may inevitably result from the alteration of intracellular levels of reactive oxygen species (ROS) [14] or glutathione (GSH) [15], and/or gene expression [16,17,18].…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, ROS and GSH are pivotal to maintaining the level of reductants and oxidants in a balanced state [25] in order to regulate oocyte maturation and normal development of zygotes. After oocytes are subjected to vitrification and warming, ROS levels are generally increased [13,14] and, conversely, GSH levels tend to decline [15,26,27]. In such situations, redox homeostasis would be perturbed, potentially weakening the quality of oocytes and reducing their developmental competence [28].…”

Section: Introductionmentioning

confidence: 99%

Melatonin Improves Parthenogenetic Development of Vitrified–Warmed Mouse Oocytes Potentially by Promoting G1/S Cell Cycle Progression

Pan

Yang

et al. 2018

IJMS

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This study aimed to investigate the effect of melatonin on the cell cycle of parthenogenetic embryos derived from vitrified mouse metaphase II (MII) oocytes. Fresh oocytes were randomly allocated into three groups: untreated (control), or vitrified by the open-pulled straw method without (Vitrification group) or with melatonin (MT) supplementation (Vitrification + MT group). After warming, oocytes were parthenogenetically activated and cultured in vitro, then the percentage of embryos in the G1/S phase, the levels of reactive oxygen species (ROS) and glutathione (GSH), and the mRNA expression of cell cycle-related genes (P53, P21 and E2F1) in zygotes and their subsequent developmental potential in vitro were evaluated. The results showed that the vitrification/warming procedures significantly decreased the frequency of the S phase, markedly increased ROS and GSH levels and the expression of P53 and P21 genes, and decreased E2F1 expression in zygotes at the G1 stage and their subsequent development into 2-cell and blastocyst stage embryos. However, when 10−9 mol/L MT was administered for the whole duration of the experiment, the frequency of the S phase in zygotes was significantly increased, while the other indicators were also significantly improved and almost recovered to the normal levels shown in the control. Thus, MT might promote G1-to-S progression via regulation of ROS, GSH and cell cycle-related genes, potentially increasing the parthenogenetic development ability of vitrified–warmed mouse oocytes.

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“…Despite this, DMSO achieves a better solid glass state upon vitrification when compared with two other commonly used cryoprotectants, acetamide or ethylene glycol (EG) (Fahy et al , 2004). To date, EG or a combination of DMSO and EG or PROH and EG are the most common cryoprotectants either commercially or in house production to be used in mouse, cattle, goat, lamb, fox, cat and human oocyte and embryo cryopreservation (Kuwayama et al , 2005; Succu et al , 2007a; Gautam et al , 2008; Berthelot-Ricou et al , 2013; Larman and Gardner, 2014; Fernandez-Gonzalez and Jewgenow, 2016; Cao et al , 2017).…”

Section: Introductionmentioning

confidence: 99%

Tolerance of lamb and mouse oocytes to cryoprotectants during vitrification

Sudiman

Lee

Ong

et al. 2018

Zygote

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SummaryMouse and lamb oocytes were vitrified with, or exposed to, different cryoprotectants and evaluated for their effects on their survival and developmental competence after in vitro fertilization (IVF) and activation treatments. Control oocytes remained untreated, whilst the remainder were exposed to three different combinations of vitrification solutions [dimethyl sulfoxide (DMSO) + ethylene glycol (EG), EG only, or propanediol (PROH) + EG] and either vitrified or left unfrozen (exposed groups). Oocytes in the control and vitrified groups underwent IVF and developmental competence was assessed to the blastocyst stage. In lambs, survival rate in vitrified oocytes was significantly lower than for oocytes in the exposed groups (P <0.05). Blastocyst development was low in vitrified oocytes compared with controls (<6% vs 38.9%, P <0.01). Parthenogenetic activation was more prevalent in vitrified lamb oocytes compared with controls (P <0.05). No evidence of zona pellucida hardening or cortical granule exocytosis could account for reduced fertilization rates in vitrified lamb oocytes. Mouse oocytes demonstrated a completely different response to lamb oocytes, with survival and parthenogenetic activation rates unaffected by the vitrification process. Treatment of mouse oocytes with DMSO + EG yielded significantly higher survival and cleavage rates than treatment with PROH + EG (87.8% and 51.7% vs 32.7% and 16.7% respectively, P <0.01), however cleavage rate for vitrified oocytes remained lower than for the controls (51.7% vs 91.7%, P <0.01) as did mean blastocyst cell number (33 ± 3.1 vs 42 ± 1.5, P <0.05). From this study, it is clear that lamb and mouse show different tolerances to cryoprotectants commonly used in vitrification procedures, and careful selection and testing of species-compatible cryoprotectants is required when vitrifying oocytes to optimize survival and embryo development.

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Effect of vitrification on meiotic maturation, mitochondrial distribution and glutathione synthesis in immature silver fox cumulus oocyte complexes

Cited by 15 publications

References 60 publications

<i>Lycium barbarum</i> polysaccharide enhances development of previously-cryopreserved murine two-cell embryos via restoration of mitochondrial function and down-regulated generation of reactive oxygen species

<i>Lycium barbarum</i> polysaccharide enhances development of previously-cryopreserved murine two-cell embryos via restoration of mitochondrial function and down-regulated generation of reactive oxygen species

Melatonin Improves Parthenogenetic Development of Vitrified–Warmed Mouse Oocytes Potentially by Promoting G1/S Cell Cycle Progression

Tolerance of lamb and mouse oocytes to cryoprotectants during vitrification

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