Effect Of Walnut Leaf, Coriander And Pomegranate On Blood Glucose And Histopathology Of Pancreas Of Alloxan Induced Diabetic Rats

Abstract

Mechanism of most of herbal used for diabetes mellitus treatment has not been well defined. This study was performed to investigate hypoglycemic effect of walnut leaf (Juglans regia L.), coriander leaf (Coriandrum sativum L.) or pomegranate seed (Punica granatum L.), and their possible role on pancreatic tissue. Diabetes mellitus was induced in 20 adult male Sprague Dawley rats and the animals were divided into four groups; three of them fed a diet supplemented with about15 gram (60 g/ kg body weight /day) of mentioned plants for 15 days. The fourth diabetic untreated group (positive control) and a non-diabetic group (negative control) received standard diet. Blood glucose was measured every day and on the last day pancreases were isolated and stained with hematoxylin & eosin (H&E) and Gomeri aldehyde fuchsin (GAF). Histomorphology and following morphometric factors were studied; Volume density of β cells, volume density of islets, percent of β cells, number of islets per square centimeter and average area of islets. The results of this study indicate that only walnut leaf was able to reduce blood glucose significantly compared with diabetic untreated group (9.029vs. 14.358 mmol/l) (P<0.05). Hypercellularity of islets tissue, increased hyperchromic nucleus in pancreatic islets of this group was obvious. Density of islets in pancreatic tissue, percent of β cells and islets size increased significantly in this group in comparison with diabetic untreated group which may signify regeneration of islets or beta cells in group received walnut leaf (P<0.05).

Effects of customary drugs and modified herbal therapy on blood glucose and total antioxidant capacity in Streptozotocin induced diabetes in albino rats: A comparative analysis

“…There is a need to continue to explore the relationship between free radicals, diabetes, and its complications, and to elucidate the mechanisms by which increased oxidative stress accelerates the development of diabetic complications, in an effort to expand treatment options. Some plants and trace elements may be proven the candidates to bring about the promising and desirable results to circumvent the day-by-day spreading menace of diabetes mellitus [10]. Coriander has been documented as a traditional While comparing the FBS levels of group C (Diabetic rats treated with coriander only) with the group A (Control), 19% increase in FBS has been noted that was not at par with the control group.…”

“…insulin, Biguanides and Sulphonyl Ureas, to bring about the promising and desirable results to circumvent the day-by-day spreading menace of diabetes mellitus with minimum or no side effects [9]. In herbal antidiabetic studies, along with a list of herbs, coriander (Coriandrum Sativum) [10] has been reported to be hypoglycemic. A study on mice found that coriander extract had both insulin-releasing and insulin-like activity [11].…”

FREKUENSI DENYUT JANTUNG DAN PERNAFASAN TIKUS PUTIH (Rattus norvegicus) DIABETES MELITUS YANG DIANESTESI DENGAN PROPOFOL

“…Kelompok (KI) diberi pelarut aloksan berupa NaCl 0,9%, sedangkan kelompok (KII) diberikan aloksan dengan dosis 150 mg/kg bobot badan. Semua perlakuan diberikan secara intraperitoneal sebanyak 0,5 ml pada masingmasing tikus tersebut (Jelodar et al, 2007). Setelah satu minggu pasca pemberian aloksan, tikus putih KII dilakukan pemeriksaan gula darah puasa untuk memastikan tikus putih menderita DM dengan menggunakan easy touch ® .…”

AKTIVITAS GLUTATION PEROKSIDASE DAN KADAR GULA DARAH TIKUS DIABETES YANG DIBERI EKSTRAK DAUN KAPULAGA (<em>Amomum cardamomum</em>)

“…Pengukuran kadar gula darah dilakukan pada hari ketujuh setelah induksi aloksan. Kisaran diabetogenik aloksan untuk memperlihatkan aktivitas toksisitas antara 180-200 mg/dl (Jelodar et al, 2007), namun untuk ditetapkan dabetes dan digunakan sebagai hewan uji diambil yang kadar glukosa darah ≥ 200 mg/dl.…”