Effect of wastewater treatment plant discharges on the functional nitrifying communities in river sediments

Montuelle, Bernard; Balandras, B.; Volat, Bernadette; Féray, Christine

doi:10.1080/714044167

Cited by 6 publications

(6 citation statements)

References 19 publications

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“…However, they also found higher nitrification rates in streams with higher NO 3 À -N concentrations . Biomass of nitrifying bacterial communities have been reported to increase downstream of WWTP's (Montuelle et al 2003). Some of the NO 3 À -N present in the water column in NBC is likely the result nitrification (Hershey et al 2004).…”

Section: Discussionmentioning

confidence: 97%

Evaluation of denitrification in an urban stream receiving wastewater effluent

2007

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Urban streams often contain elevated concentrations of nitrogen (N) which can be amplified in systems receiving effluent from wastewater treatment plants (WWTP). In this study, we evaluated the importance of denitrification in a stream draining urban Greensboro, NC, USA, using two approaches:(1) natural abundance of 15 N-NO 3 À in conjunction with background NO 3 À -N concentrations along a 7 km transect downstream of a WWTP; and (2) C 2 H 2 block experiments at three sites and at three habitat types within each site. Overall lack of a longitudinal pattern of d 15 N-NO 3 À and NO 3 À -N, combined with high concentrations of NO 3 À -N suggested that other factors were controlling NO 3 À -N flux in the study transect. However, denitrification did appear to be significant along one portion of the transect. C 2 H 2 block experiments showed that denitrification rates were much higher downstream of the WWTP compared to upstream, and showed that denitrification rates were highest in erosional and depositional areas downstream of the WWTP and in erosional areas upstream of the plant. Thus, the combination of the two methods for evaluating denitrification provided more insight into the spatial dynamics of denitrification activity than either approach alone. Denitrification appeared to be a significant sink for NO 3 À -N upstream of the WWTP, but not downstream. Approximately 46% of the total NO 3 À -N load was removed via denitrification in the upstream, urban section of the stream, while only 2.3% of NO 3 À -N was lost downstream of the plant. This result suggests that controlling NO 3 À -N loading from the plant could result in considerable improvement of downstream water quality.

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Section: Discussionmentioning

confidence: 97%

Evaluation of denitrification in an urban stream receiving wastewater effluent

2007

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“…These previous studies however, used ATU concentrations between 0.009 and 0.08 mM, which are lower than the range used in our study. Only Montuelle et al (2003) using 1.7 mM ATU suggested a potential interference in the NH + 4 quantification, although they did not not investigate nor specifically attribute the interference to the presence of ATU. ATU concentrations up to 0.08 mM are typically used to inhibit AOB activity, while higher concentrations up to 0.86 mM are relevant for archaeal (AOA) activity inhibition (Santoro and Casciotti, 2011;Hatzenpichler et al, 2008;Taylor et al, 2010).…”

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confidence: 99%

Challenges in using allylthiourea and chlorate as specific nitrification inhibitors

et al. 2017

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Allylthiourea (ATU) and chlorate (ClO) are often used to selectively inhibit nitritation and nitratation. In this work we identified challenges with use of these compounds in inhibitory assays with filter material from a biological rapid sand filter for groundwater treatment. Inhibition was investigated in continuous-flow lab-scale columns, packed with filter material from a full-scale filter and supplied with NH or NO. ATU concentrations of 0.1-0.5 mM interfered with the indophenol blue method for NH quantification leading to underestimation of the measured NH concentration. Interference was stronger at higher ATU levels and resulted in no NH detection at 0.5 mM ATU. ClO at typical concentrations for inhibition assays (1-10 mM) inhibited nitratation by less than 6%, while nitritation was instead inhibited by 91% when NH was supplied. On the other hand, nitratation was inhibited by 67-71% at 10-20 mM ClO when NO was supplied, suggesting significant nitratation inhibition at higher NO concentrations. No chlorite (ClO) was detected in the effluent, and thus we could not confirm that nitritation inhibition was caused by ClO reduction to ClO. In conclusion, ATU and ClO should be used with caution in inhibition assays, because analytical interference and poor selectivity for the targeted process may affect the experimental outcome and compromise result interpretation.

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“…Potential nitrification. It was measured according to F eray (2000) and Montuelle et al (2003). Slurry incubations of sediment were prepared in 250-mL Erlenmeyer flasks as follows: 25 mL of an NH 4 + solution (5.04 g L À1 of (NH 4 ) 2 SO 4 + 2.65 g L À1 of NaHCO 3 ) and 30 mL of ultra-pure water were added to 12.5 g of wet sediment.…”

Section: Microbial Activitymentioning

confidence: 99%

Influence of hyporheic zone characteristics on the structure and activity of microbial assemblages

et al. 2013

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International audienceThe hyporheic zone is a pivotal environment for biogeochemical processes in riverine ecosystems. However, the responses of hyporheic microbial assemblage structure and activity to environmental variables remain poorly understood. The objective of this study was to determine the influence of hyporheic zone characteristics on microbial assemblages in three French rivers (Usses, Isere and Drome). For each river, microbial structure (assemblage composition and abundance) and activity were measured at three sites with contrasting environmental characteristics (e.g. effective streambed porosity, oxygen and nutrient concentrations). Firstly, we expected that the structure and activity of the hyporheic microbial assemblage would be significantly affected by the environmental characteristics of the hyporheic zone. We also expected a strong relationship between the structure and activity of hyporheic microbial communities in the Usses River, due to greater spatial heterogeneity (i.e. greater porosity differences between the studied sites) compared with the two other rivers. Our results showed that porosity reduction, greatest in the Usses River, affected the exchanges of flows and energy through the hyporheic zone and, consequently, led to a distinct microbial assemblage composition in comparison with the two other rivers. Significant relationships between bacterial assemblage structure and activity were detected for the Usses and Drome rivers, due to the greater spatial variability in sediment and physicochemical variables observed in these two rivers than in the Isere. Hyporheic zone characteristics (sediment permeability, water chemistry, particulate organic matter) appear to be the key factors controlling microbial structure and activity in riverine environments. As the hyporheic zone is essential for biogeochemical processes, including those attenuating pollution, a better quantification of the influences of environmental factors controlling microbial activity is needed to understand and predict ecosystem processes in the hyporheic zone

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Effect of wastewater treatment plant discharges on the functional nitrifying communities in river sediments

Cited by 6 publications

References 19 publications

Evaluation of denitrification in an urban stream receiving wastewater effluent

Evaluation of denitrification in an urban stream receiving wastewater effluent

Challenges in using allylthiourea and chlorate as specific nitrification inhibitors

Influence of hyporheic zone characteristics on the structure and activity of microbial assemblages

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