Effect of water activity and temperature on growth and fumonisin B₁and B₂production by Fusarium proliferatum and F. moniliforme on maize grain

Abstract

The effect of different water activities (aw, 0.968, 0.956, 0.944, 0.925) and temperature (25 degrees C and 30 degrees C) on colonization and production of fumonisin B1 (FB1) and B2 (FB2) on sterile layers of maize by Fusarium proliferatum and F. moniliforme isolates was determined over periods of 6 weeks. Generally, both F. moniliforme and F. proliferatum grew faster with increasing aw and best at 30 degrees C. All three isolates produced more FB1 than FB2 regardless of aw or temperature. Very little FB1 and FB2 were produced at 0.925 aw, with maximum produced at 0.956 and 0.968 aw at both temperatures tested. Most FB1 and FB2 were produced by F. moniliforme (25N), followed by F. proliferatum isolates (73N and 131N). At all aw levels and both temperatures there was an increase in FB1 and FB2 concentration with time. Statistical analyses of aw, temperature, time, two- and three-way interactions showed some significant differences between isolates and FB1 and FB2 production.

Predictive modelling of temperature and water activity (solutes) on the in vitro radial growth of Botrytis cinerea Pers

“…At any temperature and whatever the solute, the B. cinerea growth rate appears to increase with the a w . Patriarca et al (2001) reported similar findings for W. sebi at 25 and 30°C, and Samapundo et al (2005), Cahagnier et al (1995), and Marín et al (1995Marín et al ( , 1999a made similar observations on F. verticilloides and F. proliferatum.…”

Production of Fumonisin Analogs by Fusarium Species

“…The fumonisin B (FB) analogs, comprising toxicologically important FB 1 , FB 2 , and FB 3 , are the most abundant naturally occurring fumonisins, with FB 1 predominating and usually being found at the highest levels (36). FB 1 typically accounts for 70 to 80% of the total fumonisins produced, while FB 2 usually makes up 15 to 25% and FB 3 usually makes up from 3 to 8% when cultured on corn or rice or in liquid medium (7,39,40). Apart from the FB series, some of the other analogs may occur in naturally contaminated maize at relatively low levels (Ͻ5% of the total fumonisins present) (48).…”

Impacto das Fumonisinas, Aflatoxinas e Ocratoxina A na Avicultura

“…Em geral, para F. verticillioides as condições ótimas de produção de fumonisinas em milho são de atividade de água (a w ) de 0,95-0,98, temperatura de 30°C e período de incubação de 4 a 6 semanas (MARÍN et al, 1995;MARÍN et al, 1999a;MARÍN et al, 1999b).…”