Ethanol is the most extensively used oxygenate for spark ignition (SI) engines. In comparison with ethanol, n-butanol exhibits a number of desirable properties for use in SI engines, which has proved to be a very promising oxygenated alternative fuel in recent years. However, the dehydration and recovery of bio-n-butanol consume extra money and energy in the acetone-n-butanolethanol (ABE) fermentation process. Hence, we focus on the research of ABE as a potential oxygenated alternative fuel in SI engines. e combustion, performance, and emission characteristics of B30, E30, ABE30 (i.e., 30 vol.% n-butanol, ethanol, and ABE blended with 70 vol.% gasoline), and G100 (pure gasoline) were compared in this study. e comparison results between B30, E30, and ABE30 at stoichiometric conditions show that ABE30 presents retarded combustion phasing, higher brake thermal efficiency, lower CO emissions, higher UHC emissions, and similar NO x emissions. In comparison with G100 under various engine loads and equivalence ratios, for the most part, ABE30 exhibits 1.4% higher brake thermal efficiency, 14% lower carbon monoxide, 9.7% lower unburned hydrocarbons, and 23.4% lower nitrogen oxides. It is indicated that ABE could be served as the oxygenate in spark ignition engine due to its capability to improve energy efficiency and reduce pollutant emissions.