Effect of Water Deficit on Rubisco and Carbonic Anhydrase Activities in Different Wheat Genotypes

Guliyev, N.M.; Bayramov, Shahniyar; Babayev, H. G.

doi:10.1007/978-1-4020-6709-9_315

Cited by 10 publications

(9 citation statements)

References 12 publications

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“…The results showed that Rubisco activity was markedly influenced by the drought stress in the flag leaf at three growth stages (Figure 3). These findings were in agreement with previous reports for wheat (Guliyev et al, 2008;Simova-Stoilova et al, 2020), rice (Gujjar et al,FIGURE 6 Malate content in the flag leaf, the sheath, the glume and the lemma under drought stress. Different small letters within the same organ meant a significant difference at the 0.05 probability level.…”

Section: Changes In Photosynthetic Enzyme Activities In Non-leaf Organssupporting

confidence: 93%

Responses of photosynthetic characteristics of oat flag leaf and spike to drought stress

et al. 2022

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Raising crops production via improving photosynthesis has always been focused. Recently excavating and increasing the photosynthetic capacity of non-leaf organs becomes an important approach to crops yield increase. Here we studied the photosynthetic characteristics of the flag leaf and the non-leaf organs including the sheath, the glume and the lemma under greenhouse. The relative water content (RWC), the stomatal characteristics, the photosynthetic pigment contents, the enzyme activities in C3 and C4 pathway and the malate content of the flag leaf and the non-leaf organs on 7, 14, 21, and 28 days after anthesis (denoted by 7DAA, 14DAA, 21DAA, and 28DAA) were determined under well-watered (CK) and water-stressed (D) treatments. Drought stress significantly reduced the RWC of the flag leaf and the non-leaf organs, while the variation of RWC in the glume and the lemma was lower than in the flag leaf. The chlorophyll a content, the chlorophyll b content, the total chlorophyll content and the xanthophyll content in the flag leaf were significantly decreased under D. However, drought stress significantly increased the photosynthetic pigment contents in the glume at the late stage (21DAA and 28DAA). In addition, the induced activities of PEPC, NADP-MDH, NADP-ME, NAD-ME, and PPDK in non-leaf organs under drought stress suggested that the C4 photosynthetic pathway in non-leaf organs compensated the limited C3 photosynthesis in the flag leaf. Non-leaf organs, in particular the glume, showed the crucial function in maintaining the stable photosynthetic performance of oat.

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Section: Changes In Photosynthetic Enzyme Activities In Non-leaf Organssupporting

confidence: 93%

Responses of photosynthetic characteristics of oat flag leaf and spike to drought stress

et al. 2022

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“…For example, its repression under Zn deficiency stress may be due to disruption of respiration/photosynthesis whilst repression under high-salinity stress may be due to regain ionic balance. On the contrary, the expression of CA transcript increased under dehydration stress as well as salt stress in the present study which corroborated with the findings of Guliyev et al (2008) as well as Gu et al (2004) who also found the upregulation of CA under dehydration in flag leaf of wheat and under salt stress of Populus euphratica, respectively.…”

Section: Expression Analysis Of Vuca1 By Q-pcrsupporting

confidence: 82%

Cloning and in silico analysis of a gene encoding a putative β-carbonic anhydrase from cowpea (Vigna unguiculata L. Walp)

Mondal

Ganie

Niraj

et al. 2013

Journal of Plant Interactions

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A gene encoding putative β-carbonic anhydrase gene (CA) from cowpea (Vigna unguiculata L. Walp.) was characterized and designated as VuCA1 (GenBank ID: JQ429799). While the genomic sequence of CA was found to be 1470 bp long with 4 introns, the open reading frame was 990 bp in length. The deduced amino acid sequence of CA contained two characteristic conserved domains, i.e. CSDSRV and EYAVLHLKVSNIVVIGHSACG, which showed high degree of homology with other β-CA genes of angiosperms. We have reported three-dimensional model of VuCA1 and quality of the predicted model was analysed with PROCHECK. Molecular docking of modeled VuCA1 revealed similar binding pockets for different substrates and products. The expression of VuCA1 transcripts was detected in different parts of the cowpea plant, with highest level observed in leaves, followed by flower buds, weak expression in stems, and the weakest in roots. The expression of VuCA1 transcripts of leaves was downregulated by zinc deficiency but upregulated by salt and dehydration stress.

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“…Drought also repressed the expression of CcCA1 coding for the carbonic anhydrase (CA) supplying CO 2 for Rubisco ( Badger and Price, 1994 ). In barley, CA activity increased in response to elevated ABA concentration, and in wheat CA activity increased during the first period of drought in flag leaves of drought-resistant genotypes but was shown to decrease at the last stage of vegetation ( Popova et al , 1996 ; Guliyev et al , 2008 ). Northern blot analysis shows that CcELIP1 was also down-regulated by drought, for all clones evaluated.…”

Section: Discussionmentioning

confidence: 99%

Differentially expressed genes and proteins upon drought acclimation in tolerant and sensitive genotypes of Coffea canephora

Marraccini¹,

Vinecky²,

Alves³

et al. 2012

Journal of Experimental Botany

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The aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a complex network of responses probably involving the abscisic signalling pathway and nitric oxide are major molecular determinants that might explain the better efficiency in controlling stomata closure and transpiration displayed by drought-tolerant clones of C. canephora.

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Effect of Water Deficit on Rubisco and Carbonic Anhydrase Activities in Different Wheat Genotypes

Cited by 10 publications

References 12 publications

Responses of photosynthetic characteristics of oat flag leaf and spike to drought stress

Responses of photosynthetic characteristics of oat flag leaf and spike to drought stress

Cloning and in silico analysis of a gene encoding a putative β-carbonic anhydrase from cowpea (Vigna unguiculata L. Walp)

Differentially expressed genes and proteins upon drought acclimation in tolerant and sensitive genotypes of Coffea canephora

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