Effect of Water Stress on Nodule Physiology and Biochemistry of a Drought Tolerant Cultivar of Common Bean (Phaseolus vulgarisL.)

Ramos, Maria José Mota

doi:10.1006/anbo.1998.0792

Cited by 104 publications

(101 citation statements)

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“…Consequently, in alfalfa nodules under stress conditions, SS activity was sufficient to sustain the production of both organic acids from Suc. The accumulation of Suc under drought stress was also reported for other legume nodules and was mainly attributed to the inhibition of SS activity (González et al, 1995;Ramos et al, 1999;Gálvez et al, 2005). Our results, especially with moderately stressed nodules, are in sharp contrast with this explanation and suggest that the accumulation of Suc in alfalfa nodules is caused by a still active import of Suc from the shoot, together with a limitation of Suc consumption in the nodules due to impairment of respiratory activity.…”

Section: Discussionsupporting

confidence: 58%

“…4B) and activity (Fig. 3), whereas concomitant decreases in SS and N 2 ase activities were observed in common bean (Ramos et al, 1999), pea (Gálvez et al, 2005), and soybean (González et al, 1995). Second, in alfalfa nodules subjected to severe drought stress, when N 2 ase was inhibited by 82%, the concentrations of malate and succinate increased by 40% and 68%, respectively (Fig.…”

Section: Discussionmentioning

confidence: 88%

“…Studies on soybean (Glycine max), common bean (Phaseolus vulgaris), and pea (Pisum sativum) have shown that the inhibitory effects may be mediated by a decrease in nodule O 2 permeability (Durand et al, 1987;Serraj and Sinclair, 1996;Ramos et al, 1999) and metabolic activity (Díaz del Castillo and Layzell, 1995). The finding that the activity of Suc synthase (SS), but not other carbon and nitrogen metabolism enzymes, rapidly declined in nodules upon imposition of stress provided strong evidence for a major role of SS in the inhibition of N 2 fixation (González et al, 1995;Gordon et al, 1997;Ramos et al, 1999). A detailed biochemical analysis of the rug4 mutant of pea, which displays severely reduced SS activity, further demonstrated an essential role of SS in symbiosis .…”

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confidence: 99%

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The Response of Carbon Metabolism and Antioxidant Defenses of Alfalfa Nodules to Drought Stress and to the Subsequent Recovery of Plants

et al. 2007

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Alfalfa (Medicago sativa) plants were exposed to drought to examine the involvement of carbon metabolism and oxidative stress in the decline of nitrogenase (N 2 ase) activity. Exposure of plants to a moderate drought (leaf water potential of 21.3 MPa) had no effect on sucrose (Suc) synthase (SS) activity, but caused inhibition of N 2 ase activity (243%), accumulation of succinate (136%) and Suc (158%), and up-regulation of genes encoding cytosolic CuZn-superoxide dismutase (SOD), plastid FeSOD, cytosolic glutathione reductase, and bacterial MnSOD and catalases B and C. Intensification of stress (22.1 MPa) decreased N 2 ase (282%) and SS (230%) activities and increased malate (140%), succinate (168%), and Suc (1435%). There was also up-regulation (mRNA) of cytosolic ascorbate peroxidase and down-regulation (mRNA) of SS, homoglutathione synthetase, and bacterial catalase A. Drought stress did not affect nifH mRNA level or leghemoglobin expression, but decreased MoFe-and Fe-proteins. Rewatering of plants led to a partial recovery of the activity (75%) and proteins (.64%) of N 2 ase, a complete recovery of Suc, and a decrease of malate (248%) relative to control. The increase in O 2 diffusion resistance, the decrease in N 2 ase-linked respiration and N 2 ase proteins, the accumulation of respiratory substrates and oxidized lipids and proteins, and the up-regulation of antioxidant genes reveal that bacteroids have their respiratory activity impaired and that oxidative stress occurs in nodules under drought conditions prior to any detectable effect on SS or leghemoglobin. We conclude that a limitation in metabolic capacity of bacteroids and oxidative damage of cellular components are contributing factors to the inhibition of N 2 ase activity in alfalfa nodules.

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Section: Discussionsupporting

confidence: 58%

Section: Discussionmentioning

confidence: 88%

mentioning

confidence: 99%

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The Response of Carbon Metabolism and Antioxidant Defenses of Alfalfa Nodules to Drought Stress and to the Subsequent Recovery of Plants

et al. 2007

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“…Hsiao & Xu (2000) reported that a decrease in soil water potential can markedly affect root hair and retard nodule growth and nitrogen fixation. In their own study, Ramos et al, (1999 andRamos et al (2003) …”

Section: Introductionmentioning

confidence: 99%

Effects of water stress on the rooting, nodulation potentials and growth of cowpea (<i>Vigna unguiculata</i> (l) walp)

Onuh¹,

Donald²

2010

Science World Journal

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Rooting, nodulation and growth ability of cowpea growing under limited water supply was investigated at the Teaching and Research Farm of the Imo State University, Owerri, Nigeria. The experiment was conducted in plastic buckets arranged in a completely Randomized Design with three replications, and treatments represented as 500ml, 1000ml, and 1500ml of water, respectively. Control treatment was represented by cowpea plants that grown with rain-fed water. The 500ml and 1000ml of water were applied two times a week to the receiving cowpea plants while the 1500ml was applied daily to the receiving cowpea. Data were collected on the number of nodules/plant, root length/plant (cm) measured at 2 and 4 wks after planting (WAP) respectively, number of leaves/plant, number of branches/plant and seed yield (kg/ha) collected data were subjected to analysis of variance at the 5% level of significance. Results showed that cowpea plants that received 500ml of water produced mean nodule of 6.0 which was significantly (p<0.05) from the mean nodule of 13.2 produced by cowpea plant that grew under rain-fed condition. Cowpea plants that received 500 and 1000ml of water produced mean roots length of 9.8cm and 19.5cm at both 2 weeks and 4 weeks after planting, respectively, which were significantly (p<0.05) longer than 5.2cm and 13.4cm mean roots length in the control, at 2 and 4 WAP. Cowpeas that received 500ml of water produced mean plant height of 117cm and number of branches of 1.0 which were significantly different (p<0.05) from the mean plants height of 47cm and number of branches of 4.1 in the control plants. Seed yield was significantly (p<0.05) higher in the control plants than in the plants that received 500 and 1000ml of water, respectively. It was concluded that root characteristics nodulation potential and seed yield of cowpea were affected by the limiting water supply. Optimal water supply for cowpeas growing in pots should therefore be studied for better irrigation management and optimal performance of cowpea.

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“…When we also carried out a principal component analysis, SNF highly correlated with stomatal conductance and CO 2 assimilation demonstrating the contribution of these two traits to SNF and vice versa (B. Fenta, unpublished results). Further, sucrose synthase activity, which is sensitive to drought, is also essential for nitrogen fixation to provide carbon supply for nodules Ramos et al 1999). Droughttolerant bean lines have sucrose synthase activity less affected under drought than susceptible lines (Ladrera et al 2007).…”

Section: Discusstionmentioning

confidence: 99%

Association of Nodule Performance Traits with Shoot Performance Traits of Common Bean Under Drought Stress

Fenta

Beebe

Rao

et al. 2014

Journal of Crop Improvement

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Effect of Water Stress on Nodule Physiology and Biochemistry of a Drought Tolerant Cultivar of Common Bean (Phaseolus vulgarisL.)

Cited by 104 publications

References 23 publications

The Response of Carbon Metabolism and Antioxidant Defenses of Alfalfa Nodules to Drought Stress and to the Subsequent Recovery of Plants

The Response of Carbon Metabolism and Antioxidant Defenses of Alfalfa Nodules to Drought Stress and to the Subsequent Recovery of Plants

Effects of water stress on the rooting, nodulation potentials and growth of cowpea (<i>Vigna unguiculata</i> (l) walp)

Association of Nodule Performance Traits with Shoot Performance Traits of Common Bean Under Drought Stress

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