Effect of Water-Surface Discharge on the Inactivation of<i>Bacillus subtilis</i>Due to Protein Lysis and DNA Damage

Kadowaki, Kazunori; Sone, Toshifumi; Kamikozawa, Takashi; Takasu, Hiroyuki

doi:10.1271/bbb.90153

Cited by 13 publications

(11 citation statements)

References 37 publications

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“…This study discusses that such DNA damage is more important for sterilizing effect, which may be caused by UV and/or H 2 O 2 generated by plasma. Also, water-surface plasma accompanying molecular oxygen (O 2 ) gas flow causes DNA degradation in B. subtilis cells [Kadowaki et al, 2009]. This study suggests that OH radical and O 3 attack cellular DNA and/or membrane as the biological targets in the plasma-induced sterilization.…”

Section: Saccharomyces Cerevisiae Staphylococcus Aureus Escherichiamentioning

confidence: 98%

“…Bacillus subtilis [Chen et al, 2008;Kadowaki et al, 2009, Sato et al, 1996. A previous study indicated that the irreversible breakdown of cell membrane in the presence of a high-electric field contributes to the sterilizing mechanism [Sale and Hamilton, 1967].…”

Section: Saccharomyces Cerevisiae Staphylococcus Aureus Escherichiamentioning

confidence: 99%

“…In fact, the application for sterilization has been also attempted from early period of the history [Hamilton and Sale, 1967]. These efforts have continued in the recent years, and there are some reports on sterilization using pulsed discharge in the water phase contaminated with various microorganisms, including S. cerevisiae, E. coli, and B. subtilis [Chen et al, 2008;Kadowaki et al, 2009;Sato et al, 1996]. Although pulsed discharge shows such effective sterilization ability, little is known about the molecular mechanism underlying this sterilization.…”

Section: Introductionmentioning

confidence: 99%

“…DNA damage was observed in B. subtilis cells treated with pulsed discharge [Kadowaki et al, 2009]. Additionally, argon gas discharge produced oxidative DNA bases such as thymine glycol (TG), 2,6-diamino-4-hydroxy-5-formamidopyrimidine (Fapy-G), 4,6-diamino-5-formamidopyrimide (Fapy-A), and 8-hydroxyadenine in solution, indicating the involvement of ROS [Su et al, 2011].…”

Section: Introductionmentioning

confidence: 99%

“…In recent years, the practical application of underwater electrical discharge has been anticipated as a strategic solution for clarifying industrial wastewater contaminated by various microorganisms and decomposing recalcitrant organic compounds [Jiang et al, 2014;Sato, 2009;Sun et al, 1997]. It has been shown that underwater electrical discharge causes bactericidal effect against S. cerevisiae, S. aureus, E. coli, and B. subtilis [Chen et al, 2008;Kadowaki et al, 2009;Sato et al, 1996].…”

Section: Introductionmentioning

confidence: 99%

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Oxidative DNA damage caused by pulsed discharge with cavitation on the bactericidal function

Kudo

Ito

Ihara

et al. 2015

J. Phys. D: Appl. Phys.

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Plasma-based techniques are expected to have practical use for wastewater purification with a potential for killing contaminated microorganisms and degrading recalcitrant materials. In the present study, we analysed oxidative DNA damage in bacterial cells treated by the plasma to unveil its mechanisms in the bactericidal process. Escherichia coli cell suspension was exposed to the plasma induced by applying an alternating-current voltage of about 1 kV with bubbling formed by water-cavitation, termed pulsed discharge with cavitation. Chromosomal DNA damage, such as double strand break (DSB) and oxidative base lesions, increased proportionally with the applied energy, as determined by electrophoretic and mass spectrometric analyses. Among the base lesions identified, the yields of 8-hydroxyguanine (8-OH-G) and 5-hydroxycytosine (5-OH-C) in chromosomal DNA increased by up to 4- and 15-fold, respectively, compared to untreated samples. The progeny DNA sequences, derived from plasmid DNA exposed to the plasma, indicated that the production rate of 5-OH-C exceeded that of 8-OH-G, as G:C to A:T transitions accounted for 65% of all base changes, but only a few G:C to T:A transversions were observed. The cell viabilities of E. coli cells decreased in direct proportion to increases in the applied energy. Therefore, the plasma-induced bactericidal mechanism appears to relate to oxidative damage caused to bacterial DNA. These results were confirmed by observing the generation of hydroxyl radicals and hydrogen peroxide molecules following the plasma exposure. We also compared our results with the plasma to those obtained with 137Cs γ-rays, as a well-known ROS generator to confirm the DNA-damaging mechanism involved.

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Section: Saccharomyces Cerevisiae Staphylococcus Aureus Escherichiamentioning

confidence: 98%

Section: Saccharomyces Cerevisiae Staphylococcus Aureus Escherichiamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Oxidative DNA damage caused by pulsed discharge with cavitation on the bactericidal function

Kudo

Ito

Ihara

et al. 2015

J. Phys. D: Appl. Phys.

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Oxidatively Generated Nucleobase Modifications in Isolated and CellularDNA

Cadet

Douki

Gasparutto

et al. 2012

Encyclopedia of Radicals in Chemistry, Biology and Materials

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The main oxidation reactions of the main pyrimidine bases (thymine and cytosine) and purine bases (adenine and guanine), and 5‐methylcytosine mediated by hydroxyl radical ( · OH) as well as one‐electron oxidants and singlet oxygen ( 1 O 2 ) leading to about 80 single‐oxidized‐type lesions are reported and the mechanism of their formation critically discussed. In addition, a few complex modifications including tandem base modifications involving either carbon‐centered radical or peroxyl radicals derived from radical oxidation of pyrimidine bases are also described. One may add modifications that arise from initial OH‐mediated hydrogen atom abstraction at C5 and C4 of the 2‐deoxyribose moiety of DNA giving rise to purine 5 ′ ,8‐cyclonucleosides and four diastereomeric aldehyde adducts to opposite cytosine bases in double‐stranded DNA, respectively. With the high level of product identification and thanks to the high sensitivity of high performance liquid chromatography (HPLC)‐electrospray ionization‐tandem mass spectrometry analysis, relevant information is gained on the formation of oxidation products in cells. Thus, 12 single and 5 complexes oxidatively generated base lesions have been detected in cellular DNA on exposure to 1 O 2 , γ rays and high‐intensity ultraviolet C (UVC) laser pulses. The information thus gained is strongly supportive of the oxidation mechanisms that were inferred from mechanistic studies on model systems in aerated aqueous solutions.

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Stimulation and Inhibition of Arabidopsis Seed Germination with Repetitive Barrier Discharges Produced by Polarity‐Reversed Voltage Pulses

Kadowaki

Kurisaka

2014

Elect Comm in Japan

Self Cite

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SUMMARY This paper presents an experimental study of control of the germination of Arabidopsis seed with a barrier discharge produced by polarity‐reversed voltage pulses. 100 ns‐width polarity‐reversed pulses were applied to the seeds between plane electrodes covered by glass barriers. The electrode configuration allowed barrier discharge propagation along the seed surface at a relatively low voltage. After the discharge treatment, the seeds were incubated for 2 or 3 days to measure the germination rate. The relationships between the cumulative input energy density into the plasma and the germination rate were investigated. The germination rate was significantly reduced at 20 J/cm3. However, it increased at cumulative energy densities up to 50 J/cm3 and then decreased again on further energy input. These results suggest a superposition of two effects, stimulation and inhibition, of the discharge treatment on the germination of Arabidopsis seed. The results of Evans Blue dyeing of the discharge‐exposed seeds indicate that necrosis of the seed coat is caused by the discharge treatment.

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Effect of Water-Surface Discharge on the Inactivation ofBacillus subtilisDue to Protein Lysis and DNA Damage

Cited by 13 publications

References 37 publications

Oxidative DNA damage caused by pulsed discharge with cavitation on the bactericidal function

Oxidative DNA damage caused by pulsed discharge with cavitation on the bactericidal function

Oxidatively Generated Nucleobase Modifications in Isolated and CellularDNA

Stimulation and Inhibition of Arabidopsis Seed Germination with Repetitive Barrier Discharges Produced by Polarity‐Reversed Voltage Pulses

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