Effect of water temperature on walleye pollock (Gadus chalcogrammus) embryos, larvae and juveniles: Survival, HSP70 expression, and physiological responses

Kim, So‐Sun; Lee, Chang-Ju; Yoo, Hae-Kyun; Choi, Jin; Byun, Soon‐Gyu; Kim, Woo Jin; Lim, Hyun-Jeong; Park, Jang-Su

doi:10.1016/j.aquaculture.2022.738136

Cited by 16 publications

(8 citation statements)

References 56 publications

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“…These results demonstrate that the temperatures at which trimerization of gHSF1 and wHSF1 occurs are closely related to the temperature of the environment. Previous studies showed that goldfish and walleye pollock cannot survive at temperatures above 38.6 40 and 19−20 °C, 41,42 respectively. Additionally, the optimal water temperature for walleye pollock growth is 8 °C.…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, the optimal water temperature for walleye pollock growth is 8 °C. 42 Thus, their survival may be associated with their respective HSF1 trimer formation temperatures. Furthermore, for HSF1 (DBD) sequences (Figure 13), coldwater fish such as walleye pollock (G. chalcogrammus), flounder (Paralichthys olivaceus), blowfish (Takifugu chinensis), Eleginops maclovinus, and Chionodraco rastropinosus contain the Phe 103 residue, whereas warmwater fish such as goldfish (C. auratus), carp (Cyprinus carpio), zebrafish (Danio rerio), and catfish contain the Tyr 103 residue (Figure 13).…”

Section: Discussionmentioning

confidence: 99%

“…By expressing HSP70 in goldfish and pollack, as well as various other fish, we found that HSF1 can bind to the DBD. 25,39,42,46,52,53 However, further analysis of the HSF1 mutations in fish is needed. Although we confirmed trimer formation of the HSF1 mutant in vitro, additional experiments should be performed to determine whether the mutant binds to the DBD in vivo.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies showed that goldfish and walleye pollock cannot survive at temperatures above 38.6 and 19–20 °C, , respectively. Additionally, the optimal water temperature for walleye pollock growth is 8 °C . Thus, their survival may be associated with their respective HSF1 trimer formation temperatures.…”

Section: Discussionmentioning

confidence: 99%

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Intermolecular Interactions between Cysteine and Aromatic Amino Acids with a Phenyl Moiety in the DNA-Binding Domain of Heat Shock Factor 1 Regulate Thermal Stress-Induced Trimerization

Lee,

Choi,

Kim

et al. 2024

Biochemistry

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In this study, we investigated the trimerization mechanism and structure of heat shock factor 1 (HSF1) using western blotting, tryptophan (Trp) fluorescence spectroscopy, and molecular modeling. First, we examined the DNA-binding domains of human (Homo sapiens), goldfish (Carassius auratus), and walleye pollock (Gadus chalcogrammus) HSF1s by mutating key residues (36 and 103) that are thought to directly affect trimer formation. Human, goldfish, and walleye pollock HSF1s contain cysteine at residue 36 but cysteine (C), tyrosine (Y), and phenylalanine (F), respectively, at residue 103. The optimal trimerization temperatures for the wild-type HSF1s of each species were found to be 42, 37, and 20 °C, respectively. Interestingly, a mutation experiment revealed that trimerization occurred at 42 °C when residue 103 was cysteine, at 37 °C when it was tyrosine, and at 20 °C when it was phenylalanine, regardless of the species. In addition, it was confirmed that when residue 103 of the three species was mutated to alanine, trimerization did not occur. This suggests that in addition to trimerization via disulfide bond formation between the cysteine residues in human HSF1, trimerization can also occur via the formation of a different type of bond between cysteine and aromatic ring residues such as tyrosine and phenylalanine. We also confirmed that at least one cysteine is required for the trimerization of HSF1s, regardless of its position (residue 36 or 103). Additionally, it was shown that the trimer formation temperature is related to growth and survival in fish.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Intermolecular Interactions between Cysteine and Aromatic Amino Acids with a Phenyl Moiety in the DNA-Binding Domain of Heat Shock Factor 1 Regulate Thermal Stress-Induced Trimerization

Lee,

Choi,

Kim

et al. 2024

Biochemistry

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“…Other studies have shown that for goldfish, more than half of their population died when the surrounding water temperature exceeded 40 °C 31 . For walleye pollock, this temperature was 19~20 °C [32][33] . This indicates that the HSF1 trimer formation.…”

Section: Discussionmentioning

confidence: 99%

Intermolecular interactions between cysteine and aromatic amino acids with phenyl moiety in the DNA-binding domain of heat shock factor 1 regulate thermal stress-induced trimerization

Lee,

Choi,

So-Sun-Kim

et al. 2023

Preprint

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In this study, we investigated the trimerization mechanism and structure of heat shock factor 1 (HSF1) in humans, goldfish, and walleye pollock at various temperatures. The trimerization of HSF1s were confirmed using western blotting using their respective antibodies. First, we examined the HSF1 DNA-binding domains of human (Homo sapiens), goldfish (Carassius auratus), and walleye pollock (Gadus chalcogrammus) by mutating key residues (36 and 103) that are thought to directly affect trimer generation. Humans, goldfish, and walleye pollock contain cysteine at residue 36, but cysteine (C), tyrosine (Y), and phenylalanine (F) at residue 103. Also, the trimer formation temperature of each species was found to be 42, 37, and 20 °C, respectively. In the mutation experiment, trimerization formed at 42 °C when residue 103 was C, at 37 °C it was Y, and at 20 °C it was F, regardless of the species. In addition, it was confirmed that when residue 103 of the three species was mutated to alanine (A), trimer was not formed. This suggest that, in addition to the previously identified C-C disulfide bonds in humans, C forms a trimer with a new type of bond with aromatic ring residues such as Y and F. Thus, HSF1 trimer formation temperature reveals the trimer creation mechanism through the fact that goldfish can have C-Y bonds at 37 °C, and walleye pollock can have C-F bonds at 20 °C. This study suggests that the trimer formation temperature and mechanism of HSF1 are regulated by the amino acid at residue 103.

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Temperature alters the oxidative and metabolic biomarkers and expression of environmental stress-related genes in chocolate mahseer (Neolissochilus hexagonolepis)

Dash

Siva

Tandel

et al. 2023

Environ Sci Pollut Res

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Effect of water temperature on walleye pollock (Gadus chalcogrammus) embryos, larvae and juveniles: Survival, HSP70 expression, and physiological responses

Cited by 16 publications

References 56 publications

Intermolecular Interactions between Cysteine and Aromatic Amino Acids with a Phenyl Moiety in the DNA-Binding Domain of Heat Shock Factor 1 Regulate Thermal Stress-Induced Trimerization

Intermolecular Interactions between Cysteine and Aromatic Amino Acids with a Phenyl Moiety in the DNA-Binding Domain of Heat Shock Factor 1 Regulate Thermal Stress-Induced Trimerization

Intermolecular interactions between cysteine and aromatic amino acids with phenyl moiety in the DNA-binding domain of heat shock factor 1 regulate thermal stress-induced trimerization

Temperature alters the oxidative and metabolic biomarkers and expression of environmental stress-related genes in chocolate mahseer (Neolissochilus hexagonolepis)

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