Effect of Whole Blood Storage on Factor VIII Recovery in Fresh Frozen Plasma and Cryoprecipitate

Abstract

Depending on logistics, whole blood has to be stored for several hours after collection. If storage time exceeds 8 h, storage has to be at 1-6 degrees C to comply with FDA regulations. In the Netherlands, however, whole blood is also stored for 12-15 h at 20-24 degrees C using butane-1,4-diol cooling devices. We compared these two storage methods for factor VIII recovery in plasma and cryoprecipitate. At laboratory scale a significantly higher factor VIII recovery was found in plasma prepared from whole blood stored at ambient temperature; in cryoprecipitate this was confirmed but differences were not significant. However, as a result of in-efficient cooling in routine procedure, no significantly different factor VIII recoveries were found in plasma prepared from whole blood stored at either of the two temperatures. From our study we conclude that overnight storage of whole blood at ambient temperature using butane-1,4-diol cooling devices has to be favoured.

Coagulation factor content of plasma produced from whole blood stored for 24 hours at ambient temperature: results from an international multicenter BEST Collaborative study

“…The loss of FVIII activity that occurs during storage of whole blood or plasma for extended periods at either 1 to 6 or 20 to 24°C has been well documented, presumably because it is known to be one of the more labile coagulation factors in plasma and as measurement of FVIII is a quality control requirement for FFP in Europe. 7,[16][17][18][19][20] In this study, we observed a 23% loss of FVIII after 24-hour storage of whole blood at ambient temperature. However, this plasma meets current EU and UK guidelines for FFP 4,5 since levels of FVIII were more than 0.70 IU/mL in 84% of units.…”

“…[27][28][29] Levels of FVIII in PF24 are lower than those in plasma produced from whole blood stored for 24 hours at ambient temperature since rather paradoxically, the loss of FVIII activity is greater when blood is stored at 4°C than 22°C. 16,17,30 This is partly due to cold precipitation of FVIII. It therefore seems reasonable to assume that the stability of coagulation factors in plasma units from this study once thawed would not be any worse than that of PF24, although this remains to be established.…”

The effect of storing whole blood at 22°C for up to 24 hours with and without rapid cooling on the quality of red cell concentrates and fresh‐frozen plasma

“…3,[5][6][7][8] However, 2,3-DPG in RCCs is undetectable after 1 to 2 weeks of storage 7,8 but recovers to within normal range within approximately 48 to 72 hours after transfusion. [9][10][11][12][13][14] FVIII activity in blood that has been cooled and then stored at 20 to 24°C for 24 hours is 80 percent of that of fresh WB 3 and is greater than WB that has been refrigerated, 15 but the activity of FVIII and other coagulation factors is also preserved in blood that has been stored at ambient temperature without active cooling. 16 Some European countries that store WB at ambient temperature before component production do not use butanediol cooling plates, 17 including some centers in France and Germany (Dr Chabanel, France, written personal communication, 2007; Dr Kretzschmar, Germany, written personal communication, 2004).…”