Effect of Wine Wastes Extracts on the Viability and Biofilm Formation of Pseudomonas aeruginosa and Staphylococcus aureus Strains

Abstract

In this work, we intended to inhibit the biofilm synthesis and the metabolism of Gram-positive and Gram-negative bacteria using two highly available wastes (stem and marc) obtained after the manufacturing of Torrontes wine at Cafayate, Argentina. Wine wastes contain a significant amount of bioactive compounds, mainly phenolic compounds, which makes them a potential source of compounds with beneficial properties to human health, as they could inhibit the virulence of pathogenic bacteria or protect the tissue against oxidative stress. Marc and stem extracts of Torrontes wine were evaluated for their ability to inhibit the metabolism and biofilm production of Pseudomonas aeruginosa and Staphylococcus aureus strains. The phytochemical composition and antioxidant activity of these extracts were also determined. The methanol and ethyl acetate extracts, which contained the highest amount of total polyphenolic, exhibited the highest scavenging capacity of ABTS and nitric oxide and the strongest Fe3+ reducing power and exhibited the highest level of inhibition of the biofilm formation and of the metabolic activity in bacterial biofilm. We also noticed a positive correlation between phenolic compounds content, the antioxidant activity, and the anti-biofilm capacity of the winemaking wastes. These results display the potentiality of wine wastes to prevent or reduce the formation of biofilm. Moreover, their abundance makes them an attractive and affordable source of antibiofilm and antioxidant agents.

Exploring the Phytochemical Composition and the Bioactive Properties of Malbec and Torrontés Wine Pomace from the Calchaquíes Valleys (Argentina) for their Sustainable Exploitation

“…These extracts demonstrated inhibition of P. aeruginosa's swarming motility, biofilm production, and metabolic activity in a biofilm environment. The antibiofilm activities showed a positive correlation with the polyphenol content of the extracts [12]. Calchaquíes Valleys wine pomaces also exhibited efficacy against Leishmania amazonensis, the responsible agent of American tegumentary leishmaniasis.…”

“…The proven antioxidant capacity of the hydroalcoholic extracts derived from Malbec and Torrontés pomace, coupled with their demonstrated antivirulence activity against pathogenic bacteria (P. aeruginosa and S. aureus) as indicated by other authors [12,13], implies a promising prospect for their use as natural preservatives in the food industry. This potential application could play a crucial role in mitigating oxidative processes.…”

“…In previous studies, white and red wine pomace extracts from the Calchaquí Valleys were found to inhibit the virulence of pathogenic bacteria (Pseudomonas aeruginosa and Staphylococcus aureus) [12,13]. These extracts demonstrated inhibition of P. aeruginosa's swarming motility, biofilm production, and metabolic activity in a biofilm environment.…”

Exploring the Phytochemical Composition and the Bioactive Properties of Malbec and Torrontés Wine Pomaces from the Calchaquíes Valleys (Argentina) for Their Sustainable Exploitation

“…In previous studies, white and red wine pomace extracts from the Calchaquí Valleys were found to inhibit the virulence of pathogenic bacteria ( Pseudomonas aeruginosa and Staphylococcus aureus ) [ 12 , 13 ]. These extracts demonstrated an inhibition of P. aeruginosa ’s swarming motility, biofilm production, and metabolic activity in a biofilm environment.…”

“…These extracts demonstrated an inhibition of P. aeruginosa ’s swarming motility, biofilm production, and metabolic activity in a biofilm environment. The antibiofilm activities showed a positive correlation with the polyphenol content of the extracts [ 12 ]. The Calchaquís Valleys’ wine pomaces also exhibited efficacy against Leishmania amazonensis , the agent responsible for American tegumentary leishmaniasis.…”