Effect of Withania somnifera on gentamicin induced renal lesions in rats

Govindappa, Prem Kumar; Gautam, Vidhi; Tripathi, Syamantak Mani; Sahni, Y.P.; Hallur, Raghavendra Lakshmana Shetty

doi:10.1016/j.bjp.2018.12.005

Cited by 35 publications

(35 citation statements)

References 29 publications

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“…This could be explained by the fact that GM accumulates in the renal tissue, leads to the loss of proximal tubular cells and Henle's loop to reabsorb water, which causes dehydration and increased urinary volume. Therefore an increase in water intake is necessary for compensation 47 .…”

Section: Effect Of Rsaqex On the Water Intake And Urine Volumementioning

confidence: 99%

Phytochemical analysis, antioxidant activity, and nephroprotective effect of the Raphanus sativus aqueous extract

et al. 2021

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In Morocco, Raphanus sativus is a widespread traditional medicinal plant used to treat various kidney diseases such as nephropathy.Objective: The present study aims to evaluate the protective effect of the R. sativus aqueous extract against the gentamicin-induced acute nephrotoxicity in rats.Methods: Rats were randomly separated into four groups (n=6; ♂/ ♀=1). The control group was treated only with distilled water (10 mL/kg; p.o). The gentamicin group was treated with distilled water (10 mL/kg; p.o) and injected intraperitoneally by the gentamicin (80 mg/kg; i.p). R. sativus groups were treated with the aqueous extracts of this plant at a dose of (200 or 400 mg/kg; p.o) and injected by the gentamicin (80 mg/kg; i.p). The plasma Creatinine, Urea, Uric Acid, Albumin, Total Protein, Alanine transaminase, Aspartate transaminase, and Calcium levels were measured. The urinary creatinine, urinary Calcium, urinary volume, water intake, creatinine clearance, body weight gain, relative right kidney weight, and kidney malondialdehydes were determined.Results: This study showed that the daily pretreatment with R. sativus aqueous extract at two doses of 200 and 400 mg/kg, p.o prevented the rats from the gentamicin-induced nephrotoxicity. Moreover, the R. sativus aqueous extract showed a high amount of polyphenols and flavonoids and a significant antioxidant activity.Conclusion: R. sativus aqueous extract, as a novel natural product, may have preventive properties against gentamicin-induced nephrotoxicity in rats. The present study describes new areas of investigation to introduce better therapeutic agents for renal disorders and dysfunction.

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Section: Effect Of Rsaqex On the Water Intake And Urine Volumementioning

confidence: 99%

Phytochemical analysis, antioxidant activity, and nephroprotective effect of the Raphanus sativus aqueous extract

et al. 2021

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“…However, long-term or high-dose treatments of antibiotics not only lead to drug resistance, but also lead to their residues in animal product. For instance, polypeptides (colistin), aminoglycosides (gentamicin, streptomycin) and tetracyclines (Doxycycline) are generally prescribed for infections caused by gram-negative bacteria [5][6][7][8]. However, these drugs may cause various Adverse Drug Reactions (ADRs), such as allergy, diarrhea, nausea and hypopsia etc.…”

Section: Introductionmentioning

confidence: 99%

Bombyx mori gloverin A2 alleviates enterotoxigenic Escherichia coli-induced inflammation and intestinal mucosa disruption

Lin

et al. 2019

Antimicrob Resist Infect Control

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Background: Enterotoxigenic Escherichia coli (ETEC) is one of the leading bacterial causes of intestinal inflammation and diarrhea. However, the ETEC is frequently resistant to common antibiotics. In this study, we explored the role of a novel antibacterial peptide Bombyx mori gloverin A2 (BMGlvA2) in alleviating ETEC-induced inflammation and intestinal epithelium disruption in mice. Methods: An ETEC-challenged mice model was used, and the ETEC-challenged mice and non-challenged mice were treated by the BMGlvA2 at different doses. Results: ETEC challenge not only elevated the concentrations of serum inflammatory cytokines such as the IL-6 and TNF-α (P < 0.01), but also elevated the concentrations of serum creatinine and urea (P < 0.05). However, BMGlvA2 attenuated the inflammatory responses by decreasing the serum inflammatory cytokines and improving the metabolisms in ETEC-challenged mice, and alleviated the ETEC-induced tissue damage in spleen. Moreover, BMGlvA2 treatment significantly elevated the duodenum villus height and decreased the crypt depth in the duodenum and ileum in ETEC-challenged mice (P < 0.05). Interestingly, BMGlvA2 improved the distribution and abundance of tightjunction protein ZO1 in duodenum and ileum epithelium after ETEC-challenge. Moreover, BMGlvA2 significantly downregulated the expression levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α) and the apoptosis-related genes (Caspase 8 and Caspase 9) in jejunal mucosa (P < 0.05) in the TETC-challenged mice. Importantly, BMGlvA2 significantly elevated the expression levels of critical genes related to mucosal barrier functions such as the mucins (MUC1 and MUC2) and glucose transporter (GLUT2) in the intestinal mucosa (P < 0.05). Conclusion: Our results suggested a novel function of the conventional antibacterial peptides, and the anti-bacterial and anti-inflammatory properties of BMGlvA2 may allow it a potential substitute for conventionally used antibiotics or drugs.

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“…Gentamicin, irrespective of cell damage, inhibits many cell membrane transporters of basolateral membranes and brush boundaries, contributing to electrolyte effects. Transport inhibition decreases cell viability as well as affects tubular reabsorption that ultimately leads to apoptosis and necrosis 25,26 . A significant reduction in potassium and calcium levels along with, at the same time, non-significant increases in sodium ions found in gentamicin therapy.…”

Section: Discussionmentioning

confidence: 99%

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2020

IJPSR

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To study the in-vitro antioxidant as well as nephroprotective potentials of Crinum asiaticum bulbs' ethanolic extract in contrast to gentamicin-induced nephrotoxicity in albino rats. Materials and Methods: The ethanolic extract of Crinum asiaticum bulbs subjected to preliminary phytochemical analysis to determine the phytoconstituents, and evaluation of antioxidant activity was done using in-vitro methods like DPPH as well as lipid peroxidation assays using ascorbic acid as standard. The in-vivo nephroprotective activity was estimated by utilizing Gentamicin induced nephrotoxicity in albino rats. Results: It was revealed by the preliminary phytochemical analysis that phenolics, tannins, steroids, terpenoids, and flavonoids components are presented. The promising results obtained by in-vitro antioxidant activity promoted us to evaluate Gentamicin induced nephrotoxicity in rats. Gentamicin impeded nephrotoxicity in rats as proved by extremely substantial (p<0.001) rise of blood urea, serum creatinine, and uric acid in Group II in comparison to Group I. Also, administration of Gentamicin in group II caused oxidative stress in rats proposed by a considerable increase in LPO level as well as a considerable decrease in GSH level. While administration of EECA in group III to V respectively caused a dose-dependent significant decrease (p<0.01) of uric acid, serum creatinine, as well as blood urea in comparison to group II. There is a significant increase in in-vivo antioxidant status (p<0.01) in catalase and GSH levels, however, there is a substantial reduction in LPO levels (p<0.01) in group III to V. Conclusion: The promising results suggested that Crinum asiaticum bulbs' ethanolic extract has nephroprotective activities in contrast to Gentamicin induced nephrotoxicity in rats. INTRODUCTION: A variety of chemicals, medicines which includes antibiotics and environmental toxins, significantly modifies the functions and structure of various tissues as well as intestine, heart, kidney, and liver are adversely affected by these 1 .

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Effect of Withania somnifera on gentamicin induced renal lesions in rats

Cited by 35 publications

References 29 publications

Phytochemical analysis, antioxidant activity, and nephroprotective effect of the Raphanus sativus aqueous extract

Phytochemical analysis, antioxidant activity, and nephroprotective effect of the Raphanus sativus aqueous extract

Bombyx mori gloverin A2 alleviates enterotoxigenic Escherichia coli-induced inflammation and intestinal mucosa disruption

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