Effect of Wood-derived Biochar Application on the Soil Microbial Community in Upland Field

Abstract

This study evaluated the effects of biochar from the pyrolysis pine on the soil microbial communities by fatty acid methyl ester (FAME) in upland field. The nutrient management for lettuce cultivation at silt loam soil was treated with four rates of biochar 0 (CFB1), 60 (CFB2), 120 (CFB3), and 240 kg 10a -1 (CFB4) with chemical fertilizer (14.3 kg 10a -1 N, 15.7 kg 10a -1 P 2 O 5 , and 12.1 kg 10a -1 K 2 O), no fertilizer (NF), and pig manure compost 2 ton 10a -1 with chemical fertilizer (CFC). Compared to the NF, the soil microbial biomass (total FAMEs), Gram-negative bacteria biomass, arbuscular mycorrhizal fungi (AMF) biomass, and total glomalin concentration were significantly increased after biochar amendments treatment, while the microbial biomasses were not significant between CFB1 and biochar treatments. CFB3 and CFB4 had a significantly lower ratio of cy19:0 to 18:1ω7c when compared with that of CFC (P < 0.05), which indicates that a decrease in microbial stress was caused by organic matter soil inputs without competition from other microbes. Community of actinomycetes in CFC soil was significantly larger than those in biochar treatment soils (P < 0.05) indicating actinomycetes are potentially responsible for the microbial community differentiation between CFC and biochar treatments in upland field.

Soil Microbial Communities and Growth of Sweet Potato (Ipomoea batatas L.) in Paddy and Upland Soils

“…고구마 재배지 토 양 입단과 수분관리를 위한 연구를 보면 바이오차를 10a당 4톤 사용한 결과 수량이 54% 증대되었다 (Liu et al, 2014). (Igalavithana et al, 2017;Yi et al, 2020). 미생물 군집을 분석하는 방법으로 미생물의 세포벽 지 방산을 분석하는 fatty acid methyl ester (FAME)와 phospholipid fatty acid (PLFA) 분석법이 있으며 (Schutter and Dick, 2000;Morris et al, 2008), DNA 증폭을 기반으로 하는 차세대 염기서열 분석법인 파이로시컨싱 등이 있다 (Ronaghi et al, 1996).…”

“…미생물 군집을 분석하는 방법으로 미생물의 세포벽 지 방산을 분석하는 fatty acid methyl ester (FAME)와 phospholipid fatty acid (PLFA) 분석법이 있으며 (Schutter and Dick, 2000;Morris et al, 2008), DNA 증폭을 기반으로 하는 차세대 염기서열 분석법인 파이로시컨싱 등이 있다 (Ronaghi et al, 1996). 파이로시컨싱은 분석 정확도는 높지만 고가의 비용이 발생되기 때문에 토양 시료가 많을 경우 저렴하고 빠르게 분석할 수 있는 FAME 방법을 많이 사용하고 있다 (Cho et al, 2019;Yang et al, 2019;Yi et al, 2020). 고구마 재배지에 대한 미생물 군집은 시비수준이 증가할수록 곰팡이 군집이 많아지며…”