Effect of Z-103 on TNB-lnduced Colitis in Rats

Abstract

Z-103 is a chelate compound consisting of zinc ion and .L-carnosine. In this study, we investigated the protective effect of Z-103 against colonic damage induced by 2,4,6-trinitrobenzene sulfonic acid (TNB) in rats. Colonic inflammation was induced by administering TNB dissolved in 50% ethanol (120 mg/ml) in male Wistar rats (total volume of 0.25 ml per rat) following a 48-hour fast. After the administration of TNB, Z-103 was given at a dose of 30 mg/kg per rat for 1 week. A second group of rats received sulfasalazine (SASP) at 300 mg/kg and a third group of rats received 30 mg/kg of ZnSO₄ for 1 week. Colonic inflammation was assessed 1 week following TNB administration. Both macro- and microscopic evaluation showed that the inflammatory responses induced by TNB were reduced by treatment with Z-103, SASP and ZnSO₄ The score (graded from 0 to 5 according to the macroscopic lesions) and colonic wet weight (distal 8 cm of the colon) were significantly decreased by treatment with Z-103, SASP and ZnSO₄ The increase in thiobarbituric acid-reactive substances in the colonic mucosa following TNB administration were inhibited in the Z-103 and SASP groups. These results suggest that Z-103 is as effective against TNB-induced colitis as SASP.

Effect of a Novel Water-Soluble Vitamin E Derivative, 2-(.ALPHA.-D-Glucopyranosyl)methyl-2,5,7,8-tetramethylchroman-6-ol, on Dextran Sulfate Sodium-Induced Colitis in Mice

“…Two lines of evidence support our hypothesis: (1) lipid peroxides significantly accumulate in the gastrointestinal mucosa during development of mucosal injury or inflammation, and (2) pretreatment with superoxide dismutase (SOD, a superoxide scavenger) or a hydroxyl radical scavenger greatly attenuates these injuries, as well as inhibiting the increase in lipid peroxides. It has also been proposed that oxygen radical-mediated lipid peroxidation is involved in the development of tissue injury in Crohn's disease and ulcerative colitis [8][9][10], as well as experimental colitis in animal models [4]. Several animal models of human inflammatory bowel disease have been studied.…”

“…Tamai et al [12] have reported a new model of colitis in rats in which the colon level of lipid peroxides was significantly increased by administration of 2,2'-azobis(2-amidinopropane)dihydrochloride (AAPH). We have also shown in a model of TNBS-induced colitis that lipid peroxides are increased significantly in the colonic mucosa [4], and treatment with SOD improves the colitis score [13]. Several studies on human inflammatory bowel disease confirm that oxidative stress is created by overproduction of reactive oxygen species or by the decrease in plasma concentrations of antioxidants, including vitamins C and E [9,[14][15][16].…”

Polaprezinc reduces the severity of radiation-induced mucositis in head and neck cancer patients

“…Zinc supplementation has been reported to decrease the severity of radiation-induced mucositis in patients with head and neck cancer (12,13). In addition, PZ, which is a chelate compound consisting of a zinc ion, L-carnosine, a β-alanine dipeptide and L-histidine, has been reported to be more effective in terms of superoxide scavenging activity compared to zinc sulfate (5). Therefore, the efficacy of PZ for radiation-induced mucositis appears to be promising.…”

“…It has been reported that PZ has antioxidant properties and scavenges free radicals (4)(5)(6)(7). We previously reported the efficacy of PZ for acute radiation proctitis in an animal model and demonstrated that PZ exerts anti-inflammatory effects following radiation exposure (8).…”

Current and Novel Treatments for Ulcerative Colitis

“…In addition, high dose of zinc has been shown to improve tight-junction permeability (209). A novel zinc compound, Z-103, a chelate compound consisting of zinc ion and L-carnosine, was utilized to assess the protective effect against colonic damage www.intechopen.com induced by TNBS in rats (210). The authors demonstrated that treatment with Z-103 reduced he inflammatory responses induced by TNBS, suggesting Z-103 may be as effective against TNBS-induced colitis.…”