Effect of zinc compounds on <i>Fusarium verticillioides</i> growth, hyphae alterations, conidia, and fumonisin production

Savi, Geovana D.; Vitorino, Vinicius Bittencourt; Bortoluzzi, Adaı́lton J.; Scussel, V. M.

doi:10.1002/jsfa.6271

Cited by 26 publications

(15 citation statements)

References 52 publications

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“…Previous research (Baider & Cohen, 2003;Helfenstein et al, 2015;Shi et al, 2006) reported positive effects of manganese-containing fungicides and zinc-containing agents on fungal diseases. Savi et al (2013) showed that zinc compounds significantly inhibited Fusarium verticillioides growth from ≥25 nmol/L concentration, and all treatments inhibited conidia production. Manganese-containing fungicides such as mancozeb could inhibit the growth of P. nicotianae, and the highest inhibition rate was 62.7% (Panek & Tomsovsky, 2017).…”

Section: Discussionmentioning

confidence: 97%

“…In other research (Helfenstein et al, 2015;Li et al, 2016), zinc-containing agents could directly act on pathogens, initiate plant defenses, and reduce the virulence of pathogenic bacteria, thereby lessening bacterial plant damage. Savi et al (2013) demonstrated that Zn compounds efficiently reduced fungal growth and fumonisin production, with the best results obtained from zinc perchlorate. At present, the inhibition mechanisms of manganese and zinc on sporangiogenesis of P. nicotianae are unclear.…”

Section: Introductionmentioning

confidence: 99%

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Effects of manganese and zinc on the growth process of Phytophthora nicotianae and the possible inhibitory mechanisms

Luo

Yao

Tan

et al. 2020

PeerJ

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Background Phytophthora nicotianae is a fungal soil-borne pathogen that damages various plant species. Mancozeb and Zineb, fungicides containing manganese (Mn) and zinc (Zn) as the main components, are widely used to control the diseases caused by Phytophthora. However, the inhibition mechanism is still unclear. The purpose of this study was to examine the effects of Mn and Zn on P. nicotianae and to determine possible inhibitory mechanisms of Mn and Zn on sporangiogenesis of P. nicotianae. Methods The mycelial growth, sporangium generation, zoosporogenesis and zoospore germination of P. nicotianae were observed under Mn and Zn treatments. The gene (csn4 and csn7) expression levels of P. nicotianae in different growth stages were examined. Csn4 and csn7 gene expression, superoxide dismutase (SOD) activity, catalase (CAT) activity and malondialdehyde (MDA) content were tested at the stage of sporangiogenesis under different Mn and Zn concentrations. Results Mycelial growth of P. nicotianae was significantly inhibited by Mn from ≥1 mg/L concentration and by Zn from ≥10 mg/L. The sporangia production, sporangia release, and zoospore germination of P. nicotianae were significantly reduced by Mn at all concentrations, while treatment with Zn from ≥0.5 mg/L concentration significantly inhibited the same processes. At the same concentration, the inhibition rate of Mn on the growth process of P. nicotianae was higher than that of Zn. The csn4 and csn7 gene transcription of P. nicotianae were significantly reduced by all treatments with Mn and Zn at the stage of sporangiogenesis. With the increase of Mn concentration, the activities of SOD and CAT increased to maxima and then decreased, and the content of MDA gradually increased during sporangiogenesis of P. nicotianae. The sporangia production of P. nicotianae was significantly positively correlated with the expression levels of the genes csn4 and csn7. Conclusion The inhibitory effect of Mn on the growth process of P. nicotianae was stronger than that of Zn, especially on sporangiogenesis and zoosporogenesis. A possible mechanism of the inhibitory effect on sporangiogenesis of P. nicotianae was that Mn and Zn acted by inhibiting the expression levels of the genes csn4 and csn7 and by affecting antioxidant enzyme activity (further resulting in lipid peroxidation) in the sporangium of P. nicotianae.

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Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Effects of manganese and zinc on the growth process of Phytophthora nicotianae and the possible inhibitory mechanisms

Luo

Yao

Tan

et al. 2020

PeerJ

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“…The difference in staining was observed under light microscope at 400x and evaluated following Semighini and Harris () and Savi et al . ().…”

Section: Methodsmentioning

confidence: 97%

“…Finally, the hyphae were washed with sterile distilled water to remove excess stain. The difference in staining was observed under light microscope at 400x and evaluated following Semighini and Harris (2010) and Savi et al (2013).…”

Section: Evans Blue Staining: Visualization Of Hyphal Alterationsmentioning

confidence: 99%

Antifungal activity ofParastrephia quadrangularis(Meyen) Cabrera extracts againstFusarium verticillioides

Ciaccio

Spotorno

Estévez

et al. 2018

Lett Appl Microbiol

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In Argentina, Fusarium verticillioides causes 'ear rot', a disease that produces important yield and nutritional quality losses in the maize producing region. This study suggests that Parastrephia quadrangularis extracts have potential for the growth inhibition against F. verticillioides M7075, and the bioactivity is reported for the first time. The results obtained will provide a starting point for discover new antimicotic candidate in natural products.

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“…Finally, the hyphae were washed with sterile distilled water to remove excess stain. The difference in staining was observed under light microscope at 400× and evaluated, following References [52,53].…”

Section: Evans Blue Staining: Visualization Of Hyphal Alterationsmentioning

confidence: 99%

In Vitro Antifungal Activity of Peltophorum dubium (Spreng.) Taub. extracts against Aspergillus flavus

Ciaccio

Catalano

López

et al. 2020

Plants

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Aspergillus flavus is a filamentous, saprophytic fungus, whose colonization occurs mainly in cereal grains and oilseeds once harvested. Under certain conditions, it could produce mycotoxins called aflatoxins, known as powerful human liver carcinogens. The aim of the present study was to describe the antifungal activity of extracts of Peltophorum dubium, a species from northern Argentina (Oriental Chaco), against A. flavus. The antifungal activities of different collection sites are reported. The extracts exhibited a minimum inhibitory concentration of 125 µg/mL, and the differences between the treatments and the inoculum control were 11 mm of P. dubium A and 10 mm of P. dubium F in colony growth. Moreover, hyphae treated with the extracts stained blue with Evans blue showed alterations in the membrane and/or cell wall, allowing the dye income. Bio-guided fractionation, High Performance Liquid Chromatography diode array ultraviolet/visible (HPLC UV/VIS DAD), and Ultra-High Performance Liquid Chromatography Electrospray Ionization Mass Spectrometry (UPLC ESI-MS) analyses were conducted to characterize the extracts and their active fractions. The HPLC UV/VIS DAD analysis allowed the determination of the presence of flavonoids (flavonols and flavones), coumarins, terpenes, and steroids. UPLC ESI/MS analysis of active fractions revealed the presence of Kaempferol, Apigenin, Naringenin, Chrysin and Daidzein.

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Effect of zinc compounds on Fusarium verticillioides growth, hyphae alterations, conidia, and fumonisin production

Abstract: Zn compounds have advantages compared to other antifungal compounds. In particular, they are non-toxic for the organism in appropriate amounts. They could be studied further as potential fungicides in agriculture.

Cited by 26 publications

References 52 publications

Effects of manganese and zinc on the growth process of Phytophthora nicotianae and the possible inhibitory mechanisms

Effects of manganese and zinc on the growth process of Phytophthora nicotianae and the possible inhibitory mechanisms

Antifungal activity ofParastrephia quadrangularis(Meyen) Cabrera extracts againstFusarium verticillioides

In Vitro Antifungal Activity of Peltophorum dubium (Spreng.) Taub. extracts against Aspergillus flavus

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