Effect of zinc deprivation on the lipid metabolism of budding yeast

Singh, Neelima; Yadav, Kamlesh K; Rajasekharan, Ram

doi:10.1007/s00294-017-0704-9

Cited by 8 publications

(5 citation statements)

References 75 publications

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“…The asterisks of " Ã "and " ÃÃ " show statistically significant differences of P , 0.05 and P , 0.01, respectively. enzymes, etc (Singh et al 2017). However, it is toxic at high intracellular levels as zinc can generate reactive oxygen species and thus trigger several biological molecules damaged to cell growth (Howlett and Avery 1997;Chrestensen et al 2000;Serero et al 2008;Pagani et al 2007).…”

Section: Discussionmentioning

confidence: 99%

“…The transition metal ion, zinc, is an essential cofactor for transcription factors and enzymes in all eukaryotic cells as well as an essential nutrient for life in all living organisms (Hambidge and Krebs 2007). However, it is toxic to cell growth when present in excess by generating reactive hydroxyl radicals and disturbing the cellular redox potential (Singh et al 2017). In addition, excess zinc competes for the binding sites in functional proteins for other metals (King et al 2000).…”

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confidence: 99%

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Identification of the Genetic Requirements for Zinc Tolerance and Toxicity inSaccharomyces cerevisiae

Zhao

Cao

Liu

et al. 2020

G3 Genes|Genomes|Genetics

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Zinc is essential for almost all living organisms, since it serves as a crucial cofactor for transcription factors and enzymes. However, it is toxic to cell growth when present in excess. The present work aims to investigate the toxicity mechanisms induced by zinc stress in yeast cells. To this end, 108 yeast single-gene deletion mutants were identified sensitive to 6 mM ZnCl 2 through a genome-wide screen. These genes were predominantly related to the biological processes of vacuolar acidification and transport, polyphosphate metabolic process, cytosolic transport, the process utilizing autophagic mechanism. A result from the measurement of intracellular zinc content showed that 64 mutants accumulated higher intracellular zinc under zinc stress than the wild-type cells. We further measured the intracellular ROS (reactive oxygen species) levels of 108 zinc-sensitive mutants treated with 3 mM ZnCl 2 . We showed that the intracellular ROS levels in 51 mutants were increased by high zinc stress, suggesting their possible involvement in regulating ROS homeostasis in response to high zinc. The results also revealed that excess zinc could generate oxidative damage and then activate the expression of several antioxidant defenses genes. Taken together, the data obtained indicated that excess zinc toxicity might be mainly due to the high intracellular zinc levels and ROS levels induced by zinc stress in yeast cells. Our current findings would provide a basis to understand the molecular mechanisms of zinc toxicity in yeast cells. KEYWORDS Saccharomycescerevisiae zinc toxicity genetic screening genomics Reactive oxygen species (ROS)

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Identification of the Genetic Requirements for Zinc Tolerance and Toxicity inSaccharomyces cerevisiae

Zhao

Cao

Liu

et al. 2020

G3 Genes|Genomes|Genetics

View full text Add to dashboard Cite

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“…The potential mechanism through which zinc affects the lipid profile is still unclear. However, it has been proved that the biological mechanism of zinc involves lipid metabolism, suppression of reactive oxygen species production, and reduction in oxidative stress ( 17 , 35 ). Therefore, appropriate zinc concentration may be essential for maintaining normal lipid levels.…”

Section: Discussionmentioning

confidence: 99%

Association between serum zinc level and lipid profiles in children with spinal muscular atrophy

Long

Feng²,

Chen³

et al. 2022

Front. Nutr.

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Background and aimsChildren with spinal muscular atrophy (SMA) have a high rate of dyslipidaemia, which is a risk factor of vital importance for cardiovascular diseases in adulthood. Studies have demonstrated that the serum zinc level is associated with lipid profiles in the general population as well as in individuals diagnosed with obesity or diabetes. The purpose of this study was to evaluate the relationship between serum zinc level and lipid profiles in children with SMA.MethodsThis cross-sectional study was launched in a tertiary children's medical center in China and involved pediatric patients with SMA under the management of a multidisciplinary team of outpatient services from July 2019 to July 2021. Anthropometric information, general clinical data, serum zinc level, lipid profiles, and body composition data were collected. Multivariate analysis was used for a thorough inquiry on the association between the serum zinc level and lipid profiles.ResultsAmong the 112 patients with SMA [median (IQR) age 5.54 years (2.75–8.29), 58.04% female], who fulfilled the inclusion criteria of the study, dyslipidaemia was detected in 60 patients (53.57%). Based on multivariable linear regression, serum zinc level was positively associated with high-density lipoprotein cholesterol (HDL-C; β = 1.63, 95% CI = 0.44–3.22) and apolipoprotein A1 (APO A1; β = 2.94, 95% CI = 0.03–5.85) levels, independently of age, sex, type, activity, percentage of body fat, and body mass index. As the serum zinc level increased by 10 μmol/L, the risk of low APO A1 levels decreased by 35% (OR = 0.65, 95% CI = 0.44–0.97) according to multivariable logistic regression analyses.ConclusionSerum zinc concentration was positively correlated with HDL-C and APO A1 levels among children with SMA. We suggest measures to correct the lower level of serum zinc to improve HDL-C and APO A1 levels.

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“…The toxic microelements destroy plasma membrane, bind in a non-specific manner with biomolecules, and interfere with the homeostasis of base metals by competing with their normal transport and buffering systems, resulting in toxic effects which impede yeast growth and metabolism [86]. The toxicity of microelements in cells can cause oxidative stress [87], base damage [88], and altered DNA repair [89], as well as inhibiting enzyme function and interfering with proliferation [90]. Additionally, the cell cycle process is also affected [91], and apoptosis or differentiation of protein function is also impaired [92].…”

Section: Detoxification Mechanism Of Trace Elements In Yeastmentioning

confidence: 99%

Accumulation and Enrichment of Trace Elements by Yeast Cells and Their Applications: A Critical Review

Sun

et al. 2022

Microorganisms

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Maintaining the homeostasis balance of trace elements is crucial for the health of organisms. Human health is threatened by diseases caused by a lack of trace elements. Saccharomyces cerevisiae has a wide and close relationship with human daily life and industrial applications. It can not only be used as fermentation products and single-cell proteins, but also as a trace elements supplement that is widely used in food, feed, and medicine. Trace-element-enriched yeast, viz., chromium-, iron-, zinc-, and selenium-enriched yeast, as an impactful microelements supplement, is more efficient, more environmentally friendly, and safer than its inorganic and organic counterparts. Over the last few decades, genetic engineering has been developing large-scaled genetic re-design and reconstruction in yeast. It is hoped that engineered yeast will include a higher concentration of trace elements. In this review, we compare the common supplement forms of several key trace elements. The mechanisms of detoxification and transport of trace elements in yeast are also reviewed thoroughly. Moreover, genes involved in the transport and detoxification of trace elements are summarized. A feasible way of metabolic engineering transformation of S. cerevisiae to produce trace-element-enriched yeast is examined. In addition, the economy, safety, and environmental protection of the engineered yeast are explored, and the future research direction of yeast enriched in trace elements is discussed.

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Effect of zinc deprivation on the lipid metabolism of budding yeast

Cited by 8 publications

References 75 publications

Identification of the Genetic Requirements for Zinc Tolerance and Toxicity inSaccharomyces cerevisiae

Identification of the Genetic Requirements for Zinc Tolerance and Toxicity inSaccharomyces cerevisiae

Association between serum zinc level and lipid profiles in children with spinal muscular atrophy

Accumulation and Enrichment of Trace Elements by Yeast Cells and Their Applications: A Critical Review

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