Effect of zinc on biochemical parameters and changes in related gene expression assessed by cDNA microarrays in pituitary of growing rats

Sun, Jiazeng; Jing, M.; Wang, Jianfeng; Zi, Nai-Tao; Fu, L J; Lu, Mingjian; Pan, Lin

doi:10.1016/j.nut.2005.07.007

Cited by 37 publications

(26 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Given that mice were at maintenance and that the level of Zn deficiency was moderate -and for a relatively short period, this response is not surprising. In growing animals, similar levels of dietary Zn have been shown to result in reduced growth rate and feed intake due to direct effects on appetite-related gene expression in the pituitary [35]. The lack of effect on BW and intake in the present study despite a 10-fold difference in dietary Zn content supports the notion that the Zn deficiency that these animals experienced was only moderate.…”

Section: Discussionsupporting

confidence: 64%

Moderate zinc restriction affects intestinal health and immune function in lipopolysaccharide-challenged mice

Peterson

Scrimgeour

McClung

et al. 2008

The Journal of Nutritional Biochemistry

View full text Add to dashboard Cite

Zinc (Zn) is an essential nutrient that affects immune function, especially within the digestive system, although the underlying mechanisms are not well understood. This study examined the effects of short-term moderate Zn restriction on intestinal health and immune function in lipopolysaccharide (LPS)-challenged mice through plasma cytokine profiling and histological evaluation of intestinal tissue sections. Adult male mice were fed with a Zn-adequate (40 ppm) or a Zn-marginal (4 ppm) diet for 4 weeks, and then a bacterial challenge was simulated by intraperitoneal injection of LPS (10 μg/g body weight [BW]) or saline (control). BW was recorded weekly, and feed intake was recorded daily over the last week. Voluntary locomotor activity was assessed 6 and 24 h after the challenge. Plasma and tissues were collected 0, 6 or 24 h after the challenge for analysis. Histological analysis of intestinal samples included evaluation of villi length and width, lamina propria (LP) width, crypt depth and intraepithelial as well as LP leukocyte numbers. Plasma was analyzed for IL-1β, IL-4, IL-6, IL-10, IL-12p40, IL-12p70, interferon gamma and tumor necrosis factor α. Diet did not affect BW and feed intake. The LPS challenge led to decreased voluntary locomotor activity (Pb.05). Moderate Zn restriction led to greater leukocyte infiltration in the LP after the LPS challenge (Pb.05) and higher plasma IL-6 and IL-10 levels 24 h after the LPS challenge (Pb.01). Results indicate that Zn status impacts intestinal responses to LPS through modulation of the cytokine response and leukocyte recruitment, and this impact is evident even with short-term (4 weeks) moderate Zn restriction.

show abstract

Section: Discussionsupporting

confidence: 64%

Moderate zinc restriction affects intestinal health and immune function in lipopolysaccharide-challenged mice

Peterson

Scrimgeour

McClung

et al. 2008

The Journal of Nutritional Biochemistry

View full text Add to dashboard Cite

show abstract

“…These appetite-related peptides are influenced by zinc during feedintake reduction (Sun et al 2006). In the present study, simultaneous feed-intake reduction and higher proinflammatory cytokines expression in the reproductive tissues have been noted.…”

Section: Discussionmentioning

confidence: 99%

High doses of dietary zinc induce cytokines, chemokines, and apoptosis in reproductive tissues during regression

et al. 2008

View full text Add to dashboard Cite

In chickens, high levels of dietary zinc cause molting, and the reproductive system undergoes complete remodeling concomitant to feather replacement. In the present study, the expression profiles of cytokines and chemokines were investigated in the ovary and oviduct of control hens and of hens induced to molt by zinc feeding. The zinc-induced feed-intake suppression, the changes in corticosterone levels, the immune cell populations in the reproductive tract, and the apoptosis of reproductive tissues were analyzed. The expression of mRNAs for interleukin-6 (IL-6), interferon-gamma (IFN-gamma), the avian ortholog of mammalian IL-8 (chCXCLi2), and a chicken MIP-1beta-like chemokine (chCCLi2) in the ovary and of mRNAs for IL-1beta, IL-6, IFN-gamma, transforming growth factor-beta2, chCXCLi2, and chCCLi2 in the oviduct were upregulated significantly during zinc-induced molting. A simultaneous feed-intake reduction was observed with higher expression of cytokines and chemokines. The results of the present investigation also suggested that the upregulation of corticosterone was closely associated with the increased expression of cytokines and chemokines. An increase in apoptosis within reproductive tissue during tissue regression was also noted. We had previously observed the upregulation of these cytokines expression in an earlier study (molting by feed withdrawal). However, the pattern and the level of expression were different among these two methods. These findings indicate that cytokines might be a common mediator of tissue regression during molting induced by diverse methods, although the pattern of induction is different. Thus, a high dose of dietary zinc seems to induce reproductive regression via the upregulation of cytokines and chemokines, the suppression of feed intake, and the increase in serum corticosterone, resulting finally in the apoptosis of reproductive tissues.

show abstract

“…Zinc is required for the normal growth and development of all animal species including humans, and its deficiency results in a reduced growth rate [24][25][26][27] . The current results also showed that zinc deficiency caused remarkable reductions in the body weight of rats.…”

Section: Discussionmentioning

confidence: 99%

Effects of Zinc on Hepatic Antioxidant Systems and the mRNA Expression Levels Assayed by cDNA Microarrays in Rats

Jing¹,

Sun²,

Zi³

et al. 2007

Ann Nutr Metab

Self Cite

View full text Add to dashboard Cite

Background: This study evaluated effects of zinc on the hepatic lipid peroxidation, antioxidant components and mRNA expression levels in rats. Methods: Three diets with different Zn levels including Zn adequacy (ZA; 34.50 mg/kg, control), Zn deficiency (ZD; 3.30 mg/kg), and Zn overdose (ZO; 345.45 mg/kg) were fed to rats for 6 weeks. The mRNA expression levels were analyzed by cDNA microarrays. Results: The body weight of rats fed the ZD diet was less (p < 0.01) than that of rats fed the ZA diet. Zn overdose elevated body weight, but the increase was not detected (p > 0.05) at week 6. Although copper and iron status in serum were declined (p < 0.01), those in liver were not affected (p > 0.05) by the high intake of zinc. The glutathione peroxidase (GPx) and glutathione (GSH) remained unchanged (p > 0.05) by zinc treatment. Rats fed the ZD diet showed reductions(p < 0.01) in the Cu-Zn superoxide dismutase (Cu-Zn SOD) and catalase (CAT) activity, and increases (p < 0.01) in the malondialdehyde and hydrogen peroxide (H₂O₂) contents. Rats fed the ZO diet particularly had higher Cu-Zn SOD (p < 0.01) activity. The mRNA expression levels of SOD were upregulated in the ZO group, and CAT was downregulated in the ZD group, while no changes in GPx mRNA levels were found after zinc treatment. Conclusion: The study suggested that zinc deficiency largely decreased body weight; zinc overdose, however, moderately stimulated growth in the early growing phase of rats. High dietary zinc did not compete with liver copper and iron status. Although Zn deficiency impaired antioxidant functions, zinc overdose hardly enhanced the antioxidant systems of animals.

show abstract

Effect of zinc on biochemical parameters and changes in related gene expression assessed by cDNA microarrays in pituitary of growing rats

Cited by 37 publications

References 40 publications

Moderate zinc restriction affects intestinal health and immune function in lipopolysaccharide-challenged mice

Moderate zinc restriction affects intestinal health and immune function in lipopolysaccharide-challenged mice

High doses of dietary zinc induce cytokines, chemokines, and apoptosis in reproductive tissues during regression

Effects of Zinc on Hepatic Antioxidant Systems and the mRNA Expression Levels Assayed by cDNA Microarrays in Rats

Contact Info

Product

Resources

About