Effect of ZnO, TiO2, Al2O3 and ZrO2 nanoparticles on wheat callus cells

Abstract

Effect of metal oxide nanoparticles on calli of two wheat varieties: Parabola (stress tolerant) and Raweta (sensitive) was studied. ZnO induced 10% larger membrane damage in Raweta calli. TiO 2 , Al 2 O 3 , and ZrO 2 caused nearly 30% greater lactate dehydrogenase leakage for Raweta compared to Parabola. UV-irradiation of samples containing ZnO particles intensified this effect. Membrane lipid peroxidation in ZnO treated Raweta calli was twice as high as in Parabola and further increased after UV-irradiation. TiO 2 , Al 2 O 3 , and ZrO 2 nanoparticles caused a 4-fold increase in malondialdehyde concentration in Raweta calli in comparison to Parabola calli. The nanoparticles studied damaged the cellular defense system by inactivating the antioxidative enzymes.

Structural and biochemical modifications of model and native membranes of human immune cells in response to the action of zinc oxide nanoparticles

“…Understanding how ZnO‐NPs works at the cellular level and interacts with the cell membrane, the first structure in contact with any substance entering the body, can contribute to the development of safer materials and greater control over their use. In the previous work, the cytotoxic effect of ZnO‐NPs on calli of two wheat varieties ( Triticum aestivum L.) was demonstrated, which was dependent on the concentration of ZnO‐NPs, their diameter and sensitivity of the tested variety (Czyżowska & Barbasz, 2019).…”

“…Understanding how ZnO-NPs works at the cellular level and interacts with the cell membrane, the first structure in contact with any substance entering the body, can contribute to the development of safer materials and greater control over their use. In the previous work, the cytotoxic effect of ZnO-NPs on calli of two wheat varieties (Triticum aestivum L.) was demonstrated, which was dependent on the concentration of ZnO-NPs, their diameter and sensitivity of the tested variety(Czyżowska & Barbasz, 2019).The two main applications that have the greatest impact on increasing the possibility of direct contact with ZnO-NPs result from their ability to protect against UV radiation and antibacterial activity.SCCS (ScientificCommittee on Consumer Safety, 2014, SCCS/1518/13) allows their use in creams in quantities up to 25%. Effective antibacterial action also requires high doses of these nanomaterials.…”

Electrodynamic assisted self-assembled fibrous hydrogel microcapsules: a novel 3Din vitroplatform for assessment of nanoparticle toxicity

“…52 The cytotoxicity of the NPs was much lower compared to a published monocultured cell type than that observed in the 3D spheroids. 53,54 Thus, as suggested, 2D systems may overestimate chemical toxicity, ascribable to the absence of 3D organization, and confer some mechanical resistance to cytoskeletal disruption.…”

Zinc Oxide Nanoparticles Damage Tobacco BY-2 Cells by Oxidative Stress Followed by Processes of Autophagy and Programmed Cell Death

“…On the contrary, these authors described changes in some biochemical parameters, such as in the glutathione S-transferase activity and in the content of protein and phytochelatin [45]. Negative effects of ZnO NPs were also studied on wheat callus cells of two varieties-Parabola (stress-tolerant) and Raweta (sensitive) and banana in vitro cultures [64,65]. Our results were in agreement with outcomes of other works that demonstrated the toxicity of NPs to a variety of plants species, e.g., soybeans, Arabidopsis, eggplants and Salicornia [33,[66][67][68].…”