2003
DOI: 10.1271/bbb.67.2269
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Effect of α,α-Dialkyl Amino Acids on the Protease Resistance of Peptides

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Cited by 70 publications
(61 citation statements)
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“…Aib Residues are known to promote the stability of the helical conformation of peptides [34], to confer to synthetic peptides a higher resistance to proteases [18], or even, in some cases, to inhibit proteolytic activity [35]. Under our experimental conditions, melittin was more sensitive to the peptidolytic activity of an Arabidopsis protein cell extract than both peptaibols, suggesting that this peptide may be more rapidly degraded in vivo.…”
mentioning
confidence: 83%
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“…Aib Residues are known to promote the stability of the helical conformation of peptides [34], to confer to synthetic peptides a higher resistance to proteases [18], or even, in some cases, to inhibit proteolytic activity [35]. Under our experimental conditions, melittin was more sensitive to the peptidolytic activity of an Arabidopsis protein cell extract than both peptaibols, suggesting that this peptide may be more rapidly degraded in vivo.…”
mentioning
confidence: 83%
“…It has been shown that Aib residues confer to peptides an increased resistance to several proteolytic enzymes [18]. Hence, the lack of effect in planta of peptides that do not contain any Aib residue (melittin, Alm-dUL) could be due to their faster degradation by plant proteases.…”
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confidence: 99%
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“…Aib is a strong helix promoting amino acid and hampers protease recognition due to steric hindrance. 46 Incorporation of a single Aib residue into the sequence of StRIP3 increases proteolytic stability by an order of magnitude (t 1/2 = 3.4 and 4.1 min, respectively, entry 4 and 5). In addition, it shifts the primary cleavage site towards the C-terminus.…”
Section: Protease Stabilitymentioning
confidence: 99%
“…S1), and we therefore used the helix-promoting α-residue Aib (Fig. 2B) at positions 17 and 30 (33,34). Aib residues can protect peptides from proteolysis, but we have observed that cyclic β-residues provide superior protection relative to Aib residues (9), and so we used Aib residues only in regions of the scaffold peptide that did not seem to tolerate cyclic β-residues (i.e., only near the central loop).…”
Section: S-s S-s S-smentioning
confidence: 99%