Effect of β-cryptoxanthin plus phytosterols on cardiovascular risk and bone turnover markers in post-menopausal women: A randomized crossover trial

Anti-proliferative effect of main dietary phytosterols and β-cryptoxanthin alone or combined in human colon cancer Caco-2 cells through cytosolic Ca+2 – and oxidative stress-induced apoptosis

“…Recently, beneficial effects have been reported from the regular consumption of a fruit-skimmed milk beverage containing β-sitosterol, campesterol and stigmasterol in combination with β-Cx. β-Cx has appeared to improve the cholesterol-lowering effect of Ps when supplied simultaneously and this combination may also be beneficial in reducing the risk of osteoporosis (Granado-Lorencio et al, 2014). Taking into account the serum concentration of β-Cx and Ps after consumption of this beverage (Alemany et al, 2013; Granado-Lorencio, Donoso-Navarro, Sánchez-Siles, Blanco-Navarro, & Pérez-Sacristán, 2011), the main goal of this study was investigating anti-proliferative effects and mechanistic aspects of the action of β-Cx and β-sitosterol, campesterol and stigmasterol (alone or in combination) in a human colon cancer cell line, at concentrations consistent with their serum levels after dietary ingestion.…”

β-Cryptoxanthin modulates the response to phytosterols in post-menopausal women carrying NPC1L1 L272L and ABCG8 A632 V polymorphisms: an exploratory study

“…Additionally, the use of a food-based approach in the present study does not exclude the potential effects of other food components (i.e. flavonoids) despite the lack of biological effect upon the intake of single components (Granado-Lorencio et al 2014). Thus, the present results should be confirmed in larger, parallel, and longer intervention trials.…”

“…Also, since no changes in lipid profile was found after consuming the beverage containing just b-Cx (Granado-Lorencio et al 2014), only the responses after the intake of beverages containing PS and PS plus b-Cx were assessed according to genetic variants. Data are presented as mean, standard error (SEM) or standard deviation (SD), and the changes in lipid markers according to the polymorphisms after the intake of each beverage were assessed using one-way ANOVA.…”

“…fibrates and statins) and serum levels of fat-soluble vitamins (A, E, D), b-Cx and biochemical and haematological profile within accepted reference ranges (except for TC and c-LDL, which were considered as inclusion criteria and a ''target'' endpoint of the intervention). Additional inclusion criteria, sample size calculations, randomization and compliance and supplements composition have been described elsewhere (Granado-Lorencio et al 2014). The study protocol was approved by the Research Ethics Committee of the Hospital Universitario Puerta de Hierro-Majadahonda (Madrid, Spain), and all subjects were informed and gave their written consent.…”

“…To assess the biological effect b-Cx and PS, three low-fat milk and fruitbased beverages were developed (Granado-Lorencio et al 2014) (1) b-Cx-enriched milk-based fruit drink, (2) PSenriched milk-based fruit drink and (3) b-Cx-enriched milkbased fruit drink with added PS. b-Cx is a major dietary provitamin A carotenoid mostly provided by citrus fruits.…”