Effect of β-glucan in water on growth performance, blood status and intestinal microbiota in tilapia under hypoxia

Souza, Felipe Pinheiro de; Lima, Ed Christian Suzuki de; Pandolfi, Victor César Freitas; Leite, Natalia Gonçalves; Furlan-Murari, Pâmela Juliana; Leal, Cindy Namie Seino; Mainardi, R.; Suphoronski, Suelen Aparecida; Favero, Leonardo Mantovani; Koch, João Fernando Albers; Pereira, Ulisses de Pádua; Lopera-Barrero, Nelson Maurício

doi:10.1016/j.aqrep.2020.100369

Cited by 31 publications

(17 citation statements)

References 66 publications

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“…Paeniclostridium was the third most abundant genus in the intestinal content samples, which was the dominant microbe in the intestinal tract of bats [ 66 ]. The genus Cetobacterium was abundant in the intestinal content samples, which was consistent with the results of previous studies on tilapia [ 14 , 75 ]. Notably, compared with the gastrointestinal mucosae, Cetobacterium was significantly enriched in the gastrointestinal contents.…”

Section: Discussionsupporting

confidence: 92%

Taxonomic and Functional Characteristics of the Gill and Gastrointestinal Microbiota and Its Correlation with Intestinal Metabolites in NEW GIFT Strain of Farmed Adult Nile Tilapia (Oreochromis niloticus)

Zhang

Lin

et al. 2021

Microorganisms

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The gill and gastrointestinal tract are primary entry routes for pathogens. The symbiotic microbiota are essential to the health, nutrition and disease of fish. Though the intestinal microbiota of Nile tilapia (Oreochromis niloticus) has been extensively studied, information on the mucosa-associated microbiota of this species, especially the gill and gastrointestinal mucosa-associated microbiota, is lacking. This study aimed to characterize the gill and gastrointestinal mucosa- and digesta-associated microbiota, as well as the intestinal metabolite profiles in the New Genetically Improved Farmed Tilapia (NEW GIFT) strain of farmed adult Nile tilapia by high-throughput sequencing and gas chromatography/mass spectrometry metabolomics. The diversity, structure, composition, and predicted function of gastrointestinal microbiota were significantly different across gastrointestinal regions and sample types (Welch t-test; p < 0.05). By comparing the mucosa- and digesta-associated microbiota, linear discriminant analysis (LDA) effect size (LEfSe) analysis revealed that Pelomonas, Ralstoniapickettii, Comamonadaceae, and Staphylococcus were significantly enriched in the mucosa-associated microbiota, whereas many bacterial taxa were significantly enriched in the digesta-associated microbiota, including Chitinophagaceae, Cetobacterium, CandidatusCompetibacter, Methyloparacoccus, and chloroplast (LDA score > 3.5). Furthermore, Undibacterium, Escherichia–Shigella, Paeniclostridium, and Cetobacterium were dominant in the intestinal contents and mucosae, whereas Sphingomonasaquatilis and Roseomonasgilardii were commonly found in the gill and stomach mucosae. The Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt2) analysis revealed that the predictive function of digesta-associated microbiota significantly differed from that of mucosa-associated microbiota (R = 0.8152, p = 0.0001). In addition, our results showed a significant interdependence between specific intestinal microbes and metabolites. Notably, the relative abundance values of several potentially beneficial microbes, including Undibacterium, Crenothrix, and Cetobacterium, were positively correlated with most intestinal metabolites, whereas the relative abundance values of some potential opportunistic pathogens, including Acinetobacter, Mycobacterium, Escherichia–Shigella, Paeniclostridium, Aeromonas, and Clostridiumsensustricto 1, were negatively correlated with most intestinal metabolites. This study revealed the characteristics of gill and gastrointestinal mucosa-associated and digesta-associated microbiota of farmed Nile tilapia and identified a close correlation between intestinal microbes and metabolites. The results serve as a basis for the effective application of targeted probiotics or prebiotics in the diet to regulate the nutrition and health of farmed tilapia.

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Section: Discussionsupporting

confidence: 92%

Taxonomic and Functional Characteristics of the Gill and Gastrointestinal Microbiota and Its Correlation with Intestinal Metabolites in NEW GIFT Strain of Farmed Adult Nile Tilapia (Oreochromis niloticus)

Zhang

Lin

et al. 2021

Microorganisms

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“…Interestingly, analysis of microbiota composition following oral gavage with β-glucans in vivo revealed no alteration of the composition, arguing that a single oral gavage with β-glucans will not alter the composition, but can alter the metabolism. In contrast to other studies investigating the effects of β-glucans on fish microbiota composition, those studies investigated the effect of constant exposure during dietary supplementation ( 27 – 33 ). Our microbiota sequencing data confirm the presence of bacterial genera commonly associated with β-glucan-fermenting capacity.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, a study investigating the effect of feeding β-glucans for 7 weeks to common carp showed, through DGGE analysis, a possible shift in bacterial species richness alongside a downregulation of il-1b expression ( 32 ). Also, addition of β-glucans to the water could affect the composition of intestinal microbiota, at least of Nile tilapia ( 33 ). Overall, possibly owing to differences in diet formulation, sampling, microbial analysis, and fish species, no consistent and distinct shift in intestinal microbial composition induced by β-glucans has been discerned.…”

Section: Introductionmentioning

confidence: 99%

β-Glucan-Induced Immuno-Modulation: A Role for the Intestinal Microbiota and Short-Chain Fatty Acids in Common Carp

et al. 2022

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Dietary supplementation of fish with β-glucans has been commonly associated with immunomodulation and generally accepted as beneficial for fish health. However, to date the exact mechanisms of immunomodulation by β-glucan supplementation in fish have remained elusive. In mammals, a clear relation between high-fibre diets, such as those including β-glucans, and diet-induced immunomodulation via intestinal microbiota and associated metabolites has been observed. In this study, first we describe by 16S rRNA sequencing the active naive microbiota of common carp intestine. Based on the abundance of the genus Bacteroides, well known for their capacity to degrade and ferment carbohydrates, we hypothesize that common carp intestinal microbiota could ferment dietary β-glucans. Indeed, two different β-glucan preparations (curdlan and MacroGard®) were both fermented in vitro, albeit with distinct fermentation dynamics and distinct production of short-chain fatty acids (SCFA). Second, we describe the potential immunomodulatory effects of the three dominant SCFAs (acetate, butyrate, and propionate) on head kidney leukocytes, showing effects on both nitric oxide production and expression of several cytokines (il-1b, il-6, tnfα, and il-10) in vitro. Interestingly, we also observed a regulation of expression of several gpr40L genes, which were recently described as putative SCFA receptors. Third, we describe how a single in vivo oral gavage of carp with MacroGard® modulated simultaneously, the expression of several pro-inflammatory genes (il-1b, il-6, tnfα), type I IFN-associated genes (tlr3.1, mx3), and three specific gpr40L genes. The in vivo observations provide indirect support to our in vitro data and the possible role of SCFAs in β-glucan-induced immunomodulation. We discuss how β-glucan-induced immunomodulatory effects can be explained, at least in part, by fermentation of MacroGard® by specific bacteria, part of the naive microbiota of common carp intestine, and how a subsequent production of SFCAs could possibly explain immunomodulation by β-glucan via SCFA receptors present on leukocytes.

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“…2020f; de Souza et al . 2020), hypoxia stress in Atlantic salmon (Rodríguez et al . 2016) and yellow croaker (Zeng et al .…”

Section: Stress Mitigation Through Dietary Additives/nutraceuticalsmentioning

confidence: 99%

Stress management in aquaculture: a review of dietary interventions

Ciji

Akhtar

2021

Reviews in Aquaculture

122

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Over the last few decades, the demand-driven growth in aquaculture has been tremendous due to development and adoption of cutting-edge technologies and production systems such as intensification and high input aquaculture. This growth, however, has witnessed an insurgence of various environmental and husbandry-related stressors posing major challenges to the aquaculture sector limiting its further expansion worldwide. These stressful conditions impair the health of cultured animals and predispose them to diseases resulting in economic losses. Hence, optimizing the stress resilience of the cultured species is paramount to ensure sustainable development of aquaculture sector. Addressing the concerns of stress mitigation in aquaculture, a plethora of research investigations have been intensively carried out all over the world targeting the management of cultured environment as well as cultured species. Among various mitigation strategies, dietary interventions appear to be pliable and a sustainable approach to develop immunocompetence and stress resistance in fish as requirement of several nutrients may increase under stressful conditions. The suboptimal and/or excessive levels of several essential and non-essential nutrients modulate the immune response and stress/disease resistance. Hence, the present review is an inclusive and informative compilation of dietary interventions for management of stress in aquaculture. This review highlights the stress-mitigating roles of different nutritive and non-nutritive compounds/additives such as amino acids, essential fatty acids, phospholipids, vitamins, minerals, carotenoids, different synthetic chemicals and biological derivatives from bacteria, yeast, fungi, algae, plants and animals as a promising stress management strategy to enhance the overall performance of the cultured species.

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Effect of β-glucan in water on growth performance, blood status and intestinal microbiota in tilapia under hypoxia

Cited by 31 publications

References 66 publications

Taxonomic and Functional Characteristics of the Gill and Gastrointestinal Microbiota and Its Correlation with Intestinal Metabolites in NEW GIFT Strain of Farmed Adult Nile Tilapia (Oreochromis niloticus)

Taxonomic and Functional Characteristics of the Gill and Gastrointestinal Microbiota and Its Correlation with Intestinal Metabolites in NEW GIFT Strain of Farmed Adult Nile Tilapia (Oreochromis niloticus)

β-Glucan-Induced Immuno-Modulation: A Role for the Intestinal Microbiota and Short-Chain Fatty Acids in Common Carp

Stress management in aquaculture: a review of dietary interventions

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