Effect of β-Mannanase and β-Mannosidase Supplementation on the Total Hydrolysis of Softwood Polysaccharides by the Talaromyces cellulolyticus Cellulase System

Abstract

Softwoods are promising lignocellulosic feedstock that provide numerous fermentable sugars via the hydrolysis of the components of cellulose and mannan-type hemicellulose such as galactoglucomannan (GGM). However, fungal cellulase systems are insufficient for the hydrolysis of softwood GGM due to the relatively low levels of mannan-degrading activities. To compensate for the deficient activities in the cellulase system, mannan-degrading enzymes were added to a cellulase preparation from Talaromyces cellulolyticus and the hydrolysis of a ball-milling-treated Douglas fir (BM-DF) was evaluated. The addition of a commercial enzyme derived from Aspergillus niger with high β-mannanase and β-mannosidase activities resulted in approximately 80 % mannose yield from BM-DF for a small protein loading amount (i.e., 1.4 mg/g substrate). Supplementation of β-mannanase and β-mannosidase purified from the commercial enzyme revealed that both enzymes were essential to improve the hydrolysis of BM-DF GGM by T. cellulolyticus cellulase. T. cellulolyticus produced inducible mannan-degrading enzymes using glucomannan as a carbon source. Supplementation of this enzyme preparation increased mannose yield from BM-DF to approximately 70 %. These results suggest that the enhancement of T. cellulolyticus β-mannosidase and β-mannanase productivity will be effective for the construction of cellulase system suitable for BM-DF hydrolysis.

Introduction of theAspergillus fumigatusα-1,2-mannosidase MsdS intoTrichoderma reeseileads to abnormal polarity and improves the ligno-cellulose degradation

“…Filamentous fungi are thought as the most efficient lignocelluloses degraders, especially T. reesei (Zhang et al, 2015; Wang et al, 2014), however, there is still an insufficient hydrolysis of lignocellulose due to the moderately low levels of mannan-degrading activity (Inoue et al, 2015). To compensate for the lower mannan-degrading activity, exogenous mannosidases have been added in the cellulase system.…”

“…To compensate for the lower mannan-degrading activity, exogenous mannosidases have been added in the cellulase system. For example, in Talaromyces cellulolyticus the approximately 80% mannose yield was increased with high β-mannanase and β-mannosidase activities when supplemented with A. niger derived commercial enzyme (Inoue et al, 2015). It has been shown that the crystal of mannan can be degraded when endo-α-1,4-mannanase from T. reesei , endo-α-1,4-mannanase from A. niger and exo-α-1,4-mannosidase from A. niger are used (Hagglund et al, 2001).…”

Introduction of the Aspergillus fumigatus α-1,2-mannosidase MsdS into Trichoderma reesei improves the ligno-cellulose degradation

“…The resulting vector was cotransformed into T. reesei with plasmid TAkulox with pyr4 gene as a selective marker. Filamentous fungi are thought as the most e cient lignocelluloses degraders, especially T. reesei [40][41], however, there is still an insu cient hydrolysis of lignocellulose due to the moderately low levels of mannan-degrading activity [42]. To compensate for the lower mannan-degrading activity, exogenous mannosidases have been added in the cellulase system.…”

“…To compensate for the lower mannan-degrading activity, exogenous mannosidases have been added in the cellulase system. For example, in Talaromyces cellulolyticus the approximately 80% mannose yield was increased with high β-mannanase and β-mannosidase activities when supplemented with A. niger derived commercial enzyme [42]. It has been shown that the crystal of mannan can be degraded when endo-α-1,4-mannanase from T. reesei, endo-α-1,4-mannanase from A. niger and exo-α-1,4-mannosidase from A. niger are used [43].…”

Two-Step Hot-Compressed Water Treatment of Douglas Fir for Efficient Total Sugar Recovery by Enzymatic Hydrolysis

“…Optimash BG (Genencor International, Palo Alto, CA, USA) and Cellulosin GM5 from Aspergillus niger (HBI Enzymes Inc., Hyogo, Japan) were used to supplement acremonium cellulase and to enhance xylan and mannan digestibility in eucalyptus and DF, respectively (Inoue et al 2008(Inoue et al , 2015.…”