Effect of β-naphthoflavone and MCPA on liver and kidney drug-metabolizing enzymes from the carp, Cyprinus carpio

Abstract

The effects of fl-naphthoflavone (B-NF) and a chlorophenoxyacetic acid herbicide (MCPA) on hepatic and renal monooxygenase activities and conjugating enzymes from immature carp (Cyprinus carpio) were studied. j3-NF increased hepatic monooxygenase activities but the patterns of differential induction generally obtained in rat liver microsomes with two series of homologous substrates, alkoxycoumarins and alkylresomfins, were not found to be similar in carp liver microsomes. On the other hand, MCPA caused no changes in oxidative metabolism, with the exception of decreased aryl hydrocarbon hydroxylase activity. Renal activities were not modified by MCPA, while b-NF treatment resulted in marked increases in monooxygenase activities with alkylresorufins as substrates. No changes were found in conjugation activities after treatment with MCPA or P-NF. These results indicate that (a) the herbicide MCPA should have no effect on drug-metabolizing enzymes from carp, and (b) the hepatic and renal monooxygenase activities of carp are responsive to fl-NF, allowing their use in monitoring water pollution.

Biochemical effects and polycyclic aromatic hydrocarbons (PAHs) in senegal sole (Solea senegalensis) from a Huelva estuary (SW Spain)

“…An increase in hepatic GST activity has been reported in several studies after fish exposure to PAHs (Bello et al, 2001;Ahmad et al, 2004;Jee and Kang, 2005;Jifa et al, 2006;Vieira et al, 2008), but most studies did not demonstrate any significant alterations (Riviere et al, 1990;Lemaire et al, 1996;Fenet et al, 1998;Van Schanke et al, 2002;Sturve et al, 2006;Sun et al, 2006;Oliveira et al, 2008) or showed a decrease of GST activity (Oikari and Jimenez, 1992;Lemaire et al, 1996;Vieira et al, 2008;Sun et al, 2008). Attempts to detect chemically induced activities of GSTs in free living fish also yielded conflicting results.…”

Hepatic biomarker responses to organic contaminants in feral chub (Leuciscus cephalus)—laboratory characterization and field study in the Sava River, Croatia

“…Following that positive experience, chub was chosen as indicator species within the framework of the European Union (EU) FP6 SARIB project (Sava River Basin: Sustainable Use, Management and Protection of Resources) dedicated to the development of integrated tools for the assessment of ecological quality of the Sava River basin in Croatia (http://www.sarib.net/index.php?id=info). The laboratory studies on responses of fish hepatic phase I and II enzymes to the presence of organic pollutants, were performed on several cyprinid fish species, such as carp, barbell, crucian carp, and roach [13–16]. However, there are no similar literature data available for chub, making the interpretation of field results difficult, especially in determining whether a particular biomarker response is an indicator of pollution‐caused impairment or a part of homeostatic response indicating that an organism is successfully dealing with the exposure stress falling within its physiological range of cellular defense [17].…”

Morphological Deformities in Brown Bullheads Administered Dietary β-Naphthoflavone

“…The compound BNF has been reported to cause an induction of cytochrome P-448-linked aryl hydrocarbon hydroxylase (AHH) enzymes in fishes and mammals (Boobis et al 1977;Elcombe and Lech 1979;Riviere et al 1990;McKillop and Case 1991). In mammals, AHH induction is mediated by the binding of aromatic hydrocarbons to an imraceilular protein, the Ah (aromatic hydrocarbon) receptor (Poland and Knutson 1982;Safe 1986).…”